Overexpression of GmProT1 and GmProT2 increases tolerance to drought and salt stresses in transgenic Arabidopsis
The proline transporter protein (ProT) plays an important role in protective stress responses in various plants. However, its function in abiotic stress responses in soybean (Glycine max) remains obscure. In the present study, two soybean ProT genes, namely GmProT1 and GmProT2, were isolated by homo...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2016-08-01
|
Series: | Journal of Integrative Agriculture |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311915612886 |
id |
doaj-a0d058b602864722845eb7440ac66766 |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Na GUO Dong XUE Wei ZHANG Jin-ming ZHAO Chen-chen XUE Qiang YAN Jin-yan XUE Hai-tang WANG Yu-mei ZHANG Han XING |
spellingShingle |
Na GUO Dong XUE Wei ZHANG Jin-ming ZHAO Chen-chen XUE Qiang YAN Jin-yan XUE Hai-tang WANG Yu-mei ZHANG Han XING Overexpression of GmProT1 and GmProT2 increases tolerance to drought and salt stresses in transgenic Arabidopsis Journal of Integrative Agriculture Glycine max, salt and drought stresses GmProT1 GmProT2 |
author_facet |
Na GUO Dong XUE Wei ZHANG Jin-ming ZHAO Chen-chen XUE Qiang YAN Jin-yan XUE Hai-tang WANG Yu-mei ZHANG Han XING |
author_sort |
Na GUO |
title |
Overexpression of GmProT1 and GmProT2 increases tolerance to drought and salt stresses in transgenic Arabidopsis |
title_short |
Overexpression of GmProT1 and GmProT2 increases tolerance to drought and salt stresses in transgenic Arabidopsis |
title_full |
Overexpression of GmProT1 and GmProT2 increases tolerance to drought and salt stresses in transgenic Arabidopsis |
title_fullStr |
Overexpression of GmProT1 and GmProT2 increases tolerance to drought and salt stresses in transgenic Arabidopsis |
title_full_unstemmed |
Overexpression of GmProT1 and GmProT2 increases tolerance to drought and salt stresses in transgenic Arabidopsis |
title_sort |
overexpression of gmprot1 and gmprot2 increases tolerance to drought and salt stresses in transgenic arabidopsis |
publisher |
Elsevier |
series |
Journal of Integrative Agriculture |
issn |
2095-3119 |
publishDate |
2016-08-01 |
description |
The proline transporter protein (ProT) plays an important role in protective stress responses in various plants. However, its function in abiotic stress responses in soybean (Glycine max) remains obscure. In the present study, two soybean ProT genes, namely GmProT1 and GmProT2, were isolated by homologous cloning. GmProT1 and GmProT2 encode polypeptides of 435 and 433 amino acids, respectively. The GmProT1 and GmProT2 proteins showed high similarity to other ProT proteins. GmProT1 and GmProT2 transcripts were detected in different soybean tissues including roots, stems, leaves, flowers, and developmental seeds, and during diverse developmental stages. GmProT1 was strongly expressed in seeds 35 days after flowering. Quantitative real-time PCR analysis showed that the two genes were highly expressed in leaves and could be strongly induced in response to salt and drought conditions and ABA treatment. Transgenic Arabidopsis thaliana plants overexpressing the two genes were generated, which showed that GmProT genes attenuate damage from salt and drought stress. In addition, transgenic Arabidopsis plants accumulated proline in response to salt and osmotic stress. Transcription levels of salinity-responsive gene (RD29B and S0S3) and drought-induced gene (CDPK1) were higher in the transgenic lines than that of wild type plants. Our work provides evidence that GmProT genes function in the response to abiotic stresses and may affect the synthesis and response system of proline. |
topic |
Glycine max, salt and drought stresses GmProT1 GmProT2 |
url |
http://www.sciencedirect.com/science/article/pii/S2095311915612886 |
work_keys_str_mv |
AT naguo overexpressionofgmprot1andgmprot2increasestolerancetodroughtandsaltstressesintransgenicarabidopsis AT dongxue overexpressionofgmprot1andgmprot2increasestolerancetodroughtandsaltstressesintransgenicarabidopsis AT weizhang overexpressionofgmprot1andgmprot2increasestolerancetodroughtandsaltstressesintransgenicarabidopsis AT jinmingzhao overexpressionofgmprot1andgmprot2increasestolerancetodroughtandsaltstressesintransgenicarabidopsis AT chenchenxue overexpressionofgmprot1andgmprot2increasestolerancetodroughtandsaltstressesintransgenicarabidopsis AT qiangyan overexpressionofgmprot1andgmprot2increasestolerancetodroughtandsaltstressesintransgenicarabidopsis AT jinyanxue overexpressionofgmprot1andgmprot2increasestolerancetodroughtandsaltstressesintransgenicarabidopsis AT haitangwang overexpressionofgmprot1andgmprot2increasestolerancetodroughtandsaltstressesintransgenicarabidopsis AT yumeizhang overexpressionofgmprot1andgmprot2increasestolerancetodroughtandsaltstressesintransgenicarabidopsis AT hanxing overexpressionofgmprot1andgmprot2increasestolerancetodroughtandsaltstressesintransgenicarabidopsis |
_version_ |
1721390839678107648 |
spelling |
doaj-a0d058b602864722845eb7440ac667662021-06-08T04:37:17ZengElsevierJournal of Integrative Agriculture2095-31192016-08-0115817271743Overexpression of GmProT1 and GmProT2 increases tolerance to drought and salt stresses in transgenic ArabidopsisNa GUO0Dong XUE1Wei ZHANG2Jin-ming ZHAO3Chen-chen XUE4Qiang YAN5Jin-yan XUE6Hai-tang WANG7Yu-mei ZHANG8Han XING9National Center for Soybean Improvement/Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture/State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, P.R. China; GUO Na, Mobile: +86-13913393081National Center for Soybean Improvement/Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture/State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, P.R. China; XUE DongNational Center for Soybean Improvement/Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture/State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaNational Center for Soybean Improvement/Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture/State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaNational Center for Soybean Improvement/Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture/State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaNational Center for Soybean Improvement/Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture/State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaNational Center for Soybean Improvement/Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture/State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaNational Center for Soybean Improvement/Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture/State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaInstitute of Crop Sciences, Fujian Academy of Agricultural Sciences, Fuzhou 350013, P.R. ChinaNational Center for Soybean Improvement/Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture/State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, P.R. China; Correspondence XING Han, Tel/Fax: +86-25-84399526The proline transporter protein (ProT) plays an important role in protective stress responses in various plants. However, its function in abiotic stress responses in soybean (Glycine max) remains obscure. In the present study, two soybean ProT genes, namely GmProT1 and GmProT2, were isolated by homologous cloning. GmProT1 and GmProT2 encode polypeptides of 435 and 433 amino acids, respectively. The GmProT1 and GmProT2 proteins showed high similarity to other ProT proteins. GmProT1 and GmProT2 transcripts were detected in different soybean tissues including roots, stems, leaves, flowers, and developmental seeds, and during diverse developmental stages. GmProT1 was strongly expressed in seeds 35 days after flowering. Quantitative real-time PCR analysis showed that the two genes were highly expressed in leaves and could be strongly induced in response to salt and drought conditions and ABA treatment. Transgenic Arabidopsis thaliana plants overexpressing the two genes were generated, which showed that GmProT genes attenuate damage from salt and drought stress. In addition, transgenic Arabidopsis plants accumulated proline in response to salt and osmotic stress. Transcription levels of salinity-responsive gene (RD29B and S0S3) and drought-induced gene (CDPK1) were higher in the transgenic lines than that of wild type plants. Our work provides evidence that GmProT genes function in the response to abiotic stresses and may affect the synthesis and response system of proline.http://www.sciencedirect.com/science/article/pii/S2095311915612886Glycine max,salt and drought stressesGmProT1GmProT2 |