Effect of glycogen synthase kinase 3β on treatment of corticosteroneinduced depression in mice treated with Xiaobuxintang-2
Objective: Xiaobuxintang-2 (XBXT-2) has antidepressant effects, but the underlying mechanism is still unclear. In this study, we used the corticosterone-induced depression mouse model to study the antidepressant effect of XBXT-2and its underlying mechanisms. Methods: A mouse model of depression w...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Editorial Board of Journal of Hainan Medical University
2020-03-01
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| Series: | Journal of Hainan Medical University |
| Subjects: | |
| Online Access: | http://www.hnykdxxb.com/PDF/202006/01.pdf |
| Summary: | Objective: Xiaobuxintang-2 (XBXT-2) has antidepressant effects, but the underlying
mechanism is still unclear. In this study, we used the corticosterone-induced depression mouse
model to study the antidepressant effect of XBXT-2and its underlying mechanisms. Methods:
A mouse model of depression was induced by corticosterone. The mice were divided into 5
groups: (i) control group, (ii) corticosterone group (CORT), (iii) corticosterone + XBXT-2
(CORT + XBXT-2) group, (iv) corticosterone + XBXT-2+ lentiviral empty group (CORT +
XBXT-2 + no-load), (v) corticosterone + XBXT-2+ lentivirus GSK3β Overexpression group
(CORT + XBXT-2 + GSK3β). The expression level of GSK3β in the hippocampus was
detected by immunoblotting, and the depression status of the mice was evaluated by forced
swimming test and tail suspension test. Results: The GSK3β lentivirus induced the high
expression of GSK3β in the hippocampus of mice, and the mRNA and protein levels were
significantly increased compared with the control group. The immobility time is significantly
increased in corticosterone injection-induced depression model mice (CORT group), and
XBXT-2 can effectively reduce the immobility time of depression model mice. Overexpression
of GFP empty lentivirus did not affect mouse behavior, whereas overexpression of GSK3β
significantly increased immobility time in depression model mice according to forced
swimming and tail suspension experiments. Conclusion: High expression of GSK3β in the
hippocampus of mice can inhibit the therapeutic effect of XBXT-2 on the corticosteroneinduced
depression in mice |
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| ISSN: | 1007-1237 1007-1237 |