Assignment of Individuals to the Iranian Native Dog Populations Using Microsatellite Markers

• Main Authors: Mahdiyeh Montazeri, Ali Akbar Masoudi, Rasoul Vaez Torshizi, Damoun Allahyar Khan Khorasani
• Format: Article
• Language: fas
• Published: Shahid Bahonar University of Kerman 2014-08-01
• Series: مجله بیوتکنولوژی کشاورزی
• Subjects: individuals assignment; genetic diversity; microsatellite markers; iranian dog native
• Online Access: https://jab.uk.ac.ir/article_1320_b9bc07ab2ab31957f1e265dbca011329.pdf
• ISSN: 2228-6705; 2228-6500

Assignment test is applied to identify the origin of a specific individual, population differentiation and medical forensic cases. Therefore, this study conducted to analyze some genetic variation criteria, comparison of the different assignment methods and assignment of individuals to eight populations of Iranian native dogs using 13 autosomal  microsatellite markers (C26.73320; CXX.6727,10,18; FH20609,20; FH20169,20; FH2790; FH2795; FH2914; FH3053; REN59H07; REN87O21; REN126A15; REN144M10 and REN86G15). The blood and tissue samples were taken from the dogs and total DNAs of the samples were extracted using salting out or enzymatic digestion methods. The results indicated that the least and the most diversity for Sangsari population (0.53) and Kurdish population (0.72), respectively. The individual’s assignment was done using 7 different methods. Among the methods base on the likelihood, the Baudouin and Lebrun method and among the methods base on the genetic distance, Nei minimum method showed the highest accuracy. Totally, markers used in this study could assign the individuals to their source population with 73 % accuracy.