Trichinella pseudospiralis in a wolverine (Gulo gulo) from the Canadian North
Species of Trichinella are a globally distributed assemblage of nematodes, often with distinct host ranges, which include people, domestic, and wild animals. Trichinella spp. are important in northern Canada, where dietary habits of people and methods of meat preparation (drying, smoking, fermenting...
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Format: | Article |
Language: | English |
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Elsevier
2019-08-01
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Series: | International Journal for Parasitology: Parasites and Wildlife |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2213224419300732 |
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doaj-a0f0065006e9477facbe19b690522628 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rajnish Sharma Peter Thompson Brett Elkin Robert Mulders Marsha Branigan Jodie Pongracz Brent Wagner Brad Scandrett Eric Hoberg Benjamin Rosenthal Emily Jenkins |
spellingShingle |
Rajnish Sharma Peter Thompson Brett Elkin Robert Mulders Marsha Branigan Jodie Pongracz Brent Wagner Brad Scandrett Eric Hoberg Benjamin Rosenthal Emily Jenkins Trichinella pseudospiralis in a wolverine (Gulo gulo) from the Canadian North International Journal for Parasitology: Parasites and Wildlife |
author_facet |
Rajnish Sharma Peter Thompson Brett Elkin Robert Mulders Marsha Branigan Jodie Pongracz Brent Wagner Brad Scandrett Eric Hoberg Benjamin Rosenthal Emily Jenkins |
author_sort |
Rajnish Sharma |
title |
Trichinella pseudospiralis in a wolverine (Gulo gulo) from the Canadian North |
title_short |
Trichinella pseudospiralis in a wolverine (Gulo gulo) from the Canadian North |
title_full |
Trichinella pseudospiralis in a wolverine (Gulo gulo) from the Canadian North |
title_fullStr |
Trichinella pseudospiralis in a wolverine (Gulo gulo) from the Canadian North |
title_full_unstemmed |
Trichinella pseudospiralis in a wolverine (Gulo gulo) from the Canadian North |
title_sort |
trichinella pseudospiralis in a wolverine (gulo gulo) from the canadian north |
publisher |
Elsevier |
series |
International Journal for Parasitology: Parasites and Wildlife |
issn |
2213-2244 |
publishDate |
2019-08-01 |
description |
Species of Trichinella are a globally distributed assemblage of nematodes, often with distinct host ranges, which include people, domestic, and wild animals. Trichinella spp. are important in northern Canada, where dietary habits of people and methods of meat preparation (drying, smoking, fermenting as well as raw) increase the risk posed by these foodborne zoonotic parasites. Outbreaks in the arctic and subarctic regions of Canada and the United States are generally attributed to T. nativa (T2) or the T6 genotype, when genetic characterization is performed. We report the discovery of Trichinella pseudospiralis (T4), a non-encapsulated species, in a wolverine (Gulo gulo) from the Northwest Territories of Canada. This parasite has been previously reported elsewhere from both mammals and carnivorous birds, but our findings represent new host and geographic records for T. pseudospiralis. Multiplex PCR and sequencing of fragments of Cytochrome Oxidase Subunit I (COI) and D3 rDNA confirmed the identification. Phylogenetically, Canadian isolates linked with each other and others derived from Palearctic or Neotropical regions, but not elsewhere in the Nearctic (continental USA). We suggest that migratory birds might have played a role in the dispersal of this pathogen 1000's of km to northwestern Canada. Wolverines are not typically consumed by humans, and thus should not pose a direct food safety risk for trichinellosis. However, the current finding suggests that they may serve as an indicator of a broader distribution for T. pseudospiralis. Along with infection risk already recognized for T. nativa and Trichinella T6, our observations emphasize the need for further studies using molecular diagnostics and alternative methods to clarify if this is a solitary case, or if T. pseudospiralis and other freeze susceptible species of Trichinella (such as T. spiralis) circulate more broadly in wildlife in Canada, and elsewhere. Keywords: Trichinella pseudospiralis, Wolverine, Canada, Palearctic, Neotropical |
url |
http://www.sciencedirect.com/science/article/pii/S2213224419300732 |
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doaj-a0f0065006e9477facbe19b6905226282020-11-25T00:43:12ZengElsevierInternational Journal for Parasitology: Parasites and Wildlife2213-22442019-08-019274280Trichinella pseudospiralis in a wolverine (Gulo gulo) from the Canadian NorthRajnish Sharma0Peter Thompson1Brett Elkin2Robert Mulders3Marsha Branigan4Jodie Pongracz5Brent Wagner6Brad Scandrett7Eric Hoberg8Benjamin Rosenthal9Emily Jenkins10Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, S7N 5B4, Canada; Corresponding author.USDA-Agricultural Research Service, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, 10300 Baltimore Avenue, Beltsville, MD, 20705, USAEnvironment and Natural Resources, Government of the Northwest Territories, 600, 5102-50th Avenue, Yellowknife, NT, X1A 3S8, CanadaEnvironment and Natural Resources, Government of the Northwest Territories, 600, 5102-50th Avenue, Yellowknife, NT, X1A 3S8, CanadaEnvironment and Natural Resources, Government of the Northwest Territories, P.O. Box 2749, Shell Lake, Inuvik, NT, X0E 0T0, CanadaEnvironment and Natural Resources, Government of the Northwest Territories, P.O. Box 2749, Shell Lake, Inuvik, NT, X0E 0T0, CanadaDepartment of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, S7N 5B4, CanadaCentre for Food-borne and Animal Parasitology, Canadian Food Inspection Agency, Saskatoon Laboratory, 116 Veterinary Road, Saskatoon, Saskatchewan, S7N 2R3, CanadaMuseum of Southwestern Biology and Department of Biology, University of New Mexico, Albuquerque, NM, 87131-0001, USAUSDA-Agricultural Research Service, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, 10300 Baltimore Avenue, Beltsville, MD, 20705, USADepartment of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, S7N 5B4, CanadaSpecies of Trichinella are a globally distributed assemblage of nematodes, often with distinct host ranges, which include people, domestic, and wild animals. Trichinella spp. are important in northern Canada, where dietary habits of people and methods of meat preparation (drying, smoking, fermenting as well as raw) increase the risk posed by these foodborne zoonotic parasites. Outbreaks in the arctic and subarctic regions of Canada and the United States are generally attributed to T. nativa (T2) or the T6 genotype, when genetic characterization is performed. We report the discovery of Trichinella pseudospiralis (T4), a non-encapsulated species, in a wolverine (Gulo gulo) from the Northwest Territories of Canada. This parasite has been previously reported elsewhere from both mammals and carnivorous birds, but our findings represent new host and geographic records for T. pseudospiralis. Multiplex PCR and sequencing of fragments of Cytochrome Oxidase Subunit I (COI) and D3 rDNA confirmed the identification. Phylogenetically, Canadian isolates linked with each other and others derived from Palearctic or Neotropical regions, but not elsewhere in the Nearctic (continental USA). We suggest that migratory birds might have played a role in the dispersal of this pathogen 1000's of km to northwestern Canada. Wolverines are not typically consumed by humans, and thus should not pose a direct food safety risk for trichinellosis. However, the current finding suggests that they may serve as an indicator of a broader distribution for T. pseudospiralis. Along with infection risk already recognized for T. nativa and Trichinella T6, our observations emphasize the need for further studies using molecular diagnostics and alternative methods to clarify if this is a solitary case, or if T. pseudospiralis and other freeze susceptible species of Trichinella (such as T. spiralis) circulate more broadly in wildlife in Canada, and elsewhere. Keywords: Trichinella pseudospiralis, Wolverine, Canada, Palearctic, Neotropicalhttp://www.sciencedirect.com/science/article/pii/S2213224419300732 |