LEDs Combined with CHO Sources and CCC Priming PLB Regeneration of <i>Phalaenopsis</i>
Throughout this study, the objective was to determine the most effective carbohydrate (CHO) sources under different light-emitting diodes (LEDs), and the impact of chlorocholine chloride (CCC), for the in vitro regeneration of the protocom-like bodies (PLBs) in <i>Phalaenopsis</i> &#...
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doaj-a10f312c475c429b9e5c61fe3abb092e2020-11-25T01:01:28ZengMDPI AGHorticulturae2311-75242019-05-01523410.3390/horticulturae5020034horticulturae5020034LEDs Combined with CHO Sources and CCC Priming PLB Regeneration of <i>Phalaenopsis</i>Hasan Mehraj0Md. Meskatul Alam1Sultana Umma Habiba2Hasan Mehbub3The United Graduate School of Agricultural Sciences, Ehime University, Ehime 790-8577, JapanThe United Graduate School of Agricultural Sciences, Ehime University, Ehime 790-8577, JapanThe United Graduate School of Agricultural Sciences, Ehime University, Ehime 790-8577, JapanFaculty of Agriculture, Kochi University, Kochi 783-8502, JapanThroughout this study, the objective was to determine the most effective carbohydrate (CHO) sources under different light-emitting diodes (LEDs), and the impact of chlorocholine chloride (CCC), for the in vitro regeneration of the protocom-like bodies (PLBs) in <i>Phalaenopsis</i> ‘Fmk02010’. We applied 15 LEDs combined with three CHO sources and five CCC concentrations in the study. Organogenesis of PLBs was very poor in maltose both for the number of PLBs and their fresh weight (FW) compared to media containing sucrose and trehalose. Sucrose was the best CHO source under the red-white (RW) LED for the in vitro organogenesis of PLBs (PLBs: 54.13; FW: 0.109 g), while trehalose was best under the blue-white (BW) LED (PLBs: 36.33, FW: 0.129 g). The red-blue-white (RBW)-trehalose combination generated a suitable number of PLBs (35.13) with the highest FW (0.167 g). CCC at 0.01, 0.1, and 1 mgL<sup>−1</sup>CCC had no effect on PLB formation or FW, but 10 mg L<sup>−1</sup> reduced both. RW-sucrose, BW-trehalose, and RBW-trehalose were the best combinations for PLB organogenesis. The addition of low concentrations of CCC in the plant culture medium are unnecessary.https://www.mdpi.com/2311-7524/5/2/34protocorm-like bodieslight-emitting diodetrehalosemaltoseCCCcorrelationgrowth retardants |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hasan Mehraj Md. Meskatul Alam Sultana Umma Habiba Hasan Mehbub |
spellingShingle |
Hasan Mehraj Md. Meskatul Alam Sultana Umma Habiba Hasan Mehbub LEDs Combined with CHO Sources and CCC Priming PLB Regeneration of <i>Phalaenopsis</i> Horticulturae protocorm-like bodies light-emitting diode trehalose maltose CCC correlation growth retardants |
author_facet |
Hasan Mehraj Md. Meskatul Alam Sultana Umma Habiba Hasan Mehbub |
author_sort |
Hasan Mehraj |
title |
LEDs Combined with CHO Sources and CCC Priming PLB Regeneration of <i>Phalaenopsis</i> |
title_short |
LEDs Combined with CHO Sources and CCC Priming PLB Regeneration of <i>Phalaenopsis</i> |
title_full |
LEDs Combined with CHO Sources and CCC Priming PLB Regeneration of <i>Phalaenopsis</i> |
title_fullStr |
LEDs Combined with CHO Sources and CCC Priming PLB Regeneration of <i>Phalaenopsis</i> |
title_full_unstemmed |
LEDs Combined with CHO Sources and CCC Priming PLB Regeneration of <i>Phalaenopsis</i> |
title_sort |
leds combined with cho sources and ccc priming plb regeneration of <i>phalaenopsis</i> |
publisher |
MDPI AG |
series |
Horticulturae |
issn |
2311-7524 |
publishDate |
2019-05-01 |
description |
Throughout this study, the objective was to determine the most effective carbohydrate (CHO) sources under different light-emitting diodes (LEDs), and the impact of chlorocholine chloride (CCC), for the in vitro regeneration of the protocom-like bodies (PLBs) in <i>Phalaenopsis</i> ‘Fmk02010’. We applied 15 LEDs combined with three CHO sources and five CCC concentrations in the study. Organogenesis of PLBs was very poor in maltose both for the number of PLBs and their fresh weight (FW) compared to media containing sucrose and trehalose. Sucrose was the best CHO source under the red-white (RW) LED for the in vitro organogenesis of PLBs (PLBs: 54.13; FW: 0.109 g), while trehalose was best under the blue-white (BW) LED (PLBs: 36.33, FW: 0.129 g). The red-blue-white (RBW)-trehalose combination generated a suitable number of PLBs (35.13) with the highest FW (0.167 g). CCC at 0.01, 0.1, and 1 mgL<sup>−1</sup>CCC had no effect on PLB formation or FW, but 10 mg L<sup>−1</sup> reduced both. RW-sucrose, BW-trehalose, and RBW-trehalose were the best combinations for PLB organogenesis. The addition of low concentrations of CCC in the plant culture medium are unnecessary. |
topic |
protocorm-like bodies light-emitting diode trehalose maltose CCC correlation growth retardants |
url |
https://www.mdpi.com/2311-7524/5/2/34 |
work_keys_str_mv |
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