Polarized growth in the absence of F-actin in Saccharomyces cerevisiae exiting quiescence.
Polarity establishment and maintenance are crucial for morphogenesis and development. In budding yeast, these two intricate processes involve the superposition of regulatory loops between polarity landmarks, RHO GTPases, actin-mediated vesicles transport and endocytosis. Deciphering the chronology a...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2008-07-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC2440520?pdf=render |
id |
doaj-a12359447d0c45f5bb1d7257387ef7d7 |
---|---|
record_format |
Article |
spelling |
doaj-a12359447d0c45f5bb1d7257387ef7d72020-11-24T21:34:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-07-0137e255610.1371/journal.pone.0002556Polarized growth in the absence of F-actin in Saccharomyces cerevisiae exiting quiescence.Annelise SahinBertrand Daignan-FornierIsabelle SagotPolarity establishment and maintenance are crucial for morphogenesis and development. In budding yeast, these two intricate processes involve the superposition of regulatory loops between polarity landmarks, RHO GTPases, actin-mediated vesicles transport and endocytosis. Deciphering the chronology and the significance of each molecular step of polarized growth is therefore very challenging.We have taken advantage of the fact that yeast quiescent cells display actin bodies, a non polarized actin structure, to evaluate the role of F-actin in bud emergence. Here we show that upon exit from quiescence, actin cables are not required for the first steps of polarized growth. We further show that polarized growth can occur in the absence of actin patch-mediated endocytosis. We finally establish, using latrunculin-A, that the first steps of polarized growth do not require any F-actin containing structures. Yet, these structures are required for the formation of a bona fide daughter cell and cell cycle completion. We propose that upon exit from quiescence in the absence of F-actin, secretory vesicles randomly reach the plasma membrane but preferentially dock and fuse where polarity cues are localized, this being sufficient to trigger polarized growth.http://europepmc.org/articles/PMC2440520?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Annelise Sahin Bertrand Daignan-Fornier Isabelle Sagot |
spellingShingle |
Annelise Sahin Bertrand Daignan-Fornier Isabelle Sagot Polarized growth in the absence of F-actin in Saccharomyces cerevisiae exiting quiescence. PLoS ONE |
author_facet |
Annelise Sahin Bertrand Daignan-Fornier Isabelle Sagot |
author_sort |
Annelise Sahin |
title |
Polarized growth in the absence of F-actin in Saccharomyces cerevisiae exiting quiescence. |
title_short |
Polarized growth in the absence of F-actin in Saccharomyces cerevisiae exiting quiescence. |
title_full |
Polarized growth in the absence of F-actin in Saccharomyces cerevisiae exiting quiescence. |
title_fullStr |
Polarized growth in the absence of F-actin in Saccharomyces cerevisiae exiting quiescence. |
title_full_unstemmed |
Polarized growth in the absence of F-actin in Saccharomyces cerevisiae exiting quiescence. |
title_sort |
polarized growth in the absence of f-actin in saccharomyces cerevisiae exiting quiescence. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2008-07-01 |
description |
Polarity establishment and maintenance are crucial for morphogenesis and development. In budding yeast, these two intricate processes involve the superposition of regulatory loops between polarity landmarks, RHO GTPases, actin-mediated vesicles transport and endocytosis. Deciphering the chronology and the significance of each molecular step of polarized growth is therefore very challenging.We have taken advantage of the fact that yeast quiescent cells display actin bodies, a non polarized actin structure, to evaluate the role of F-actin in bud emergence. Here we show that upon exit from quiescence, actin cables are not required for the first steps of polarized growth. We further show that polarized growth can occur in the absence of actin patch-mediated endocytosis. We finally establish, using latrunculin-A, that the first steps of polarized growth do not require any F-actin containing structures. Yet, these structures are required for the formation of a bona fide daughter cell and cell cycle completion. We propose that upon exit from quiescence in the absence of F-actin, secretory vesicles randomly reach the plasma membrane but preferentially dock and fuse where polarity cues are localized, this being sufficient to trigger polarized growth. |
url |
http://europepmc.org/articles/PMC2440520?pdf=render |
work_keys_str_mv |
AT annelisesahin polarizedgrowthintheabsenceoffactininsaccharomycescerevisiaeexitingquiescence AT bertranddaignanfornier polarizedgrowthintheabsenceoffactininsaccharomycescerevisiaeexitingquiescence AT isabellesagot polarizedgrowthintheabsenceoffactininsaccharomycescerevisiaeexitingquiescence |
_version_ |
1725950596496752640 |