The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation

Background. DNA demethylation, the conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), plays important roles in diverse biological processes and multiple diseases by regulating gene expression. Methods. In this study, utilizi...

Full description

Bibliographic Details
Main Authors:	Huikang Tao, Pei Xie, Yuhang Cao, Liqi Shu, Liping Li, Junchen Chen, Guangfeng Tian, Yingliang Zhuang, Qiang Shu, Xuekun Li
Format:	Article
Language:	English
Published:	Hindawi Limited 2018-01-01
Series:	Stem Cells International
Online Access:	http://dx.doi.org/10.1155/2018/2186301

id	doaj-a130ab2ee6894f90b121f29513d1e5d9
record_format	Article
spelling	doaj-a130ab2ee6894f90b121f29513d1e5d92020-11-24T22:29:02ZengHindawi LimitedStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/21863012186301The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell DifferentiationHuikang Tao0Pei Xie1Yuhang Cao2Liqi Shu3Liping Li4Junchen Chen5Guangfeng Tian6Yingliang Zhuang7Qiang Shu8Xuekun Li9The Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, ChinaThe Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, ChinaThe Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, ChinaSchool of Medicine and Health Sciences, George Washington University, Washington, DC 20037, USAThe Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, ChinaThe Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, ChinaThe Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, ChinaThe Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, ChinaThe Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, ChinaThe Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, ChinaBackground. DNA demethylation, the conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), plays important roles in diverse biological processes and multiple diseases by regulating gene expression. Methods. In this study, utilizing DNA dot blot, immunofluorescence staining, and qRT-PCR, we studied the expression pattern of Tets, the enzymes governing DNA demethylation, and the levels of 5hmC, 5fC, and 5caC during the postnatal neuronal development of mice. Results. It was found that 5hmC, 5fC, and 5caC were highly enriched in multiple brain regions and aNSCs and displayed temporal and spatial patterns during postnatal neuronal development and the differentiation of aNSCs. Consistently, the expression of Tets also exhibited temporal and spatial patterns. Conclusion. DNA demethylation displayed dynamic features during postnatal neuronal development and the differentiation of aNSCs of mice, which could contribute to appropriate gene expression.http://dx.doi.org/10.1155/2018/2186301
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Huikang Tao Pei Xie Yuhang Cao Liqi Shu Liping Li Junchen Chen Guangfeng Tian Yingliang Zhuang Qiang Shu Xuekun Li
spellingShingle	Huikang Tao Pei Xie Yuhang Cao Liqi Shu Liping Li Junchen Chen Guangfeng Tian Yingliang Zhuang Qiang Shu Xuekun Li The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation Stem Cells International
author_facet	Huikang Tao Pei Xie Yuhang Cao Liqi Shu Liping Li Junchen Chen Guangfeng Tian Yingliang Zhuang Qiang Shu Xuekun Li
author_sort	Huikang Tao
title	The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation
title_short	The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation
title_full	The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation
title_fullStr	The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation
title_full_unstemmed	The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation
title_sort	dynamic dna demethylation during postnatal neuronal development and neural stem cell differentiation
publisher	Hindawi Limited
series	Stem Cells International
issn	1687-966X 1687-9678
publishDate	2018-01-01
description	Background. DNA demethylation, the conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), plays important roles in diverse biological processes and multiple diseases by regulating gene expression. Methods. In this study, utilizing DNA dot blot, immunofluorescence staining, and qRT-PCR, we studied the expression pattern of Tets, the enzymes governing DNA demethylation, and the levels of 5hmC, 5fC, and 5caC during the postnatal neuronal development of mice. Results. It was found that 5hmC, 5fC, and 5caC were highly enriched in multiple brain regions and aNSCs and displayed temporal and spatial patterns during postnatal neuronal development and the differentiation of aNSCs. Consistently, the expression of Tets also exhibited temporal and spatial patterns. Conclusion. DNA demethylation displayed dynamic features during postnatal neuronal development and the differentiation of aNSCs of mice, which could contribute to appropriate gene expression.
url	http://dx.doi.org/10.1155/2018/2186301
work_keys_str_mv	AT huikangtao thedynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation AT peixie thedynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation AT yuhangcao thedynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation AT liqishu thedynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation AT lipingli thedynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation AT junchenchen thedynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation AT guangfengtian thedynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation AT yingliangzhuang thedynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation AT qiangshu thedynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation AT xuekunli thedynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation AT huikangtao dynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation AT peixie dynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation AT yuhangcao dynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation AT liqishu dynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation AT lipingli dynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation AT junchenchen dynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation AT guangfengtian dynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation AT yingliangzhuang dynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation AT qiangshu dynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation AT xuekunli dynamicdnademethylationduringpostnatalneuronaldevelopmentandneuralstemcelldifferentiation
_version_	1725745111150624768

The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation

Similar Items