Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro
Tulathromycin is a relatively new semi-synthetic macrolide antibiotic, a member of the triamilide group, approved primarly for the treatment of respiratory diseases in cattle and swine. Various genotoxicological studies indicated that tulathromycin is not genotoxic, but no available publish...
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Serbian Genetics Society
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doaj-a130e0fd1527470983a6f10c3c314d052020-11-25T00:32:55ZengSerbian Genetics SocietyGenetika0534-00121820-60692015-01-0147133934810.2298/GENSR1501339M0534-00121501339MEvaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitroMaletić Jelena0Đelić Ninoslav1Radaković Milena2Maletić Milan3Lakić Nada4Kukolj Vladimir5Aleksić Nevenka6Anđelković Marko7Stanimirović Zoran8Faculty of Veterinary Medicine, BelgradeFaculty of Veterinary Medicine, BelgradeFaculty of Veterinary Medicine, BelgradeFaculty of Veterinary Medicine, BelgradeFaculty of Agriculture, BelgradeFaculty of Veterinary Medicine, BelgradeFaculty of Veterinary Medicine, BelgradeSerbian Academy of Science and Arts, BelgradeFaculty of Veterinary Medicine, BelgradeTulathromycin is a relatively new semi-synthetic macrolide antibiotic, a member of the triamilide group, approved primarly for the treatment of respiratory diseases in cattle and swine. Various genotoxicological studies indicated that tulathromycin is not genotoxic, but no available published data originate from the single-cell gel electrophoresis (Comet) assay. Therefore, the objective of this study was to examine whether it can induce primary DNA damage using in vitro Comet assay in isolated rat lymphocytes. Lymphocytes were treated with a broad spectrum of tulathromycin concentrations (from 1 to 100 µ M) and co-treatment with an antioxidant, catalase (100 IU/mL and 500 IU/mL) was performed. The highest concentrations of tulathromycin (50 and 100 µ M) caused significant increase of DNA damage in rat lymphocytes and catalase did not significantly reduce the DNA-damaging effect of tulathromycin. The results of this study indicate that tulathromycin induces genotoxic effects at high concentrations, that catalase does not exert protective effect in this case. [Projekat Ministarstva nauke Republike Srbije, br. III 46002]http://www.doiserbia.nb.rs/img/doi/0534-0012/2015/0534-00121501339M.pdfcomet assayDNA damagerat lymphocytestulathromycin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maletić Jelena Đelić Ninoslav Radaković Milena Maletić Milan Lakić Nada Kukolj Vladimir Aleksić Nevenka Anđelković Marko Stanimirović Zoran |
spellingShingle |
Maletić Jelena Đelić Ninoslav Radaković Milena Maletić Milan Lakić Nada Kukolj Vladimir Aleksić Nevenka Anđelković Marko Stanimirović Zoran Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro Genetika comet assay DNA damage rat lymphocytes tulathromycin |
author_facet |
Maletić Jelena Đelić Ninoslav Radaković Milena Maletić Milan Lakić Nada Kukolj Vladimir Aleksić Nevenka Anđelković Marko Stanimirović Zoran |
author_sort |
Maletić Jelena |
title |
Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro |
title_short |
Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro |
title_full |
Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro |
title_fullStr |
Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro |
title_full_unstemmed |
Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro |
title_sort |
evaluation of dna damage in rat lymphocytes exposed to tulathromycin in vitro |
publisher |
Serbian Genetics Society |
series |
Genetika |
issn |
0534-0012 1820-6069 |
publishDate |
2015-01-01 |
description |
Tulathromycin is a relatively new semi-synthetic macrolide antibiotic, a
member of the triamilide group, approved primarly for the treatment of
respiratory diseases in cattle and swine. Various genotoxicological studies
indicated that tulathromycin is not genotoxic, but no available published
data originate from the single-cell gel electrophoresis (Comet) assay.
Therefore, the objective of this study was to examine whether it can induce
primary DNA damage using in vitro Comet assay in isolated rat lymphocytes.
Lymphocytes were treated with a broad spectrum of tulathromycin
concentrations (from 1 to 100 µ M) and co-treatment with an antioxidant,
catalase (100 IU/mL and 500 IU/mL) was performed. The highest concentrations
of tulathromycin (50 and 100 µ M) caused significant increase of DNA damage
in rat lymphocytes and catalase did not significantly reduce the DNA-damaging
effect of tulathromycin. The results of this study indicate that
tulathromycin induces genotoxic effects at high concentrations, that catalase
does not exert protective effect in this case. [Projekat Ministarstva nauke
Republike Srbije, br. III 46002] |
topic |
comet assay DNA damage rat lymphocytes tulathromycin |
url |
http://www.doiserbia.nb.rs/img/doi/0534-0012/2015/0534-00121501339M.pdf |
work_keys_str_mv |
AT maleticjelena evaluationofdnadamageinratlymphocytesexposedtotulathromycininvitro AT đelicninoslav evaluationofdnadamageinratlymphocytesexposedtotulathromycininvitro AT radakovicmilena evaluationofdnadamageinratlymphocytesexposedtotulathromycininvitro AT maleticmilan evaluationofdnadamageinratlymphocytesexposedtotulathromycininvitro AT lakicnada evaluationofdnadamageinratlymphocytesexposedtotulathromycininvitro AT kukoljvladimir evaluationofdnadamageinratlymphocytesexposedtotulathromycininvitro AT aleksicnevenka evaluationofdnadamageinratlymphocytesexposedtotulathromycininvitro AT anđelkovicmarko evaluationofdnadamageinratlymphocytesexposedtotulathromycininvitro AT stanimiroviczoran evaluationofdnadamageinratlymphocytesexposedtotulathromycininvitro |
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