Tracking single cells in live animals using a photoconvertible near-infrared cell membrane label.
We describe a novel photoconversion technique to track individual cells in vivo using a commercial lipophilic membrane dye, DiR. We show that DiR exhibits a permanent fluorescence emission shift (photoconversion) after light exposure and does not reacquire the original color over time. Ratiometric i...
Main Authors: | Alicia L Carlson, Joji Fujisaki, Juwell Wu, Judith M Runnels, Raphaël Turcotte, Joel A Spencer, Cristina Lo Celso, David T Scadden, Terry B Strom, Charles P Lin |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3753322?pdf=render |
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