Integrative analyses of single-cell transcriptome and regulome using MAESTRO
Abstract We present Model-based AnalysEs of Transcriptome and RegulOme (MAESTRO), a comprehensive open-source computational workflow ( http://github.com/liulab-dfci/MAESTRO ) for the integrative analyses of single-cell RNA-seq (scRNA-seq) and ATAC-seq (scATAC-seq) data from multiple platforms. MAEST...
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doaj-a14be4895a4e47b89cd8a78404f17bba2020-11-25T03:37:00ZengBMCGenome Biology1474-760X2020-08-0121112810.1186/s13059-020-02116-xIntegrative analyses of single-cell transcriptome and regulome using MAESTROChenfei Wang0Dongqing Sun1Xin Huang2Changxin Wan3Ziyi Li4Ya Han5Qian Qin6Jingyu Fan7Xintao Qiu8Yingtian Xie9Clifford A. Meyer10Myles Brown11Ming Tang12Henry Long13Tao Liu14X. Shirley Liu15Department of Data Science, Dana-Farber Cancer Institute, Harvard T.H. Chan School of Public HealthClinical Translational Research Center, Shanghai Pulmonary Hospital, School of Life Science and Technology, Tongji UniversityBeijing Institute of Radiation MedicineClinical Translational Research Center, Shanghai Pulmonary Hospital, School of Life Science and Technology, Tongji UniversityClinical Translational Research Center, Shanghai Pulmonary Hospital, School of Life Science and Technology, Tongji UniversityClinical Translational Research Center, Shanghai Pulmonary Hospital, School of Life Science and Technology, Tongji UniversityClinical Translational Research Center, Shanghai Pulmonary Hospital, School of Life Science and Technology, Tongji UniversityClinical Translational Research Center, Shanghai Pulmonary Hospital, School of Life Science and Technology, Tongji UniversityCenter for Functional Cancer Epigenetics, Dana-Farber Cancer InstituteCenter for Functional Cancer Epigenetics, Dana-Farber Cancer InstituteDepartment of Data Science, Dana-Farber Cancer Institute, Harvard T.H. Chan School of Public HealthCenter for Functional Cancer Epigenetics, Dana-Farber Cancer InstituteDepartment of Data Science, Dana-Farber Cancer Institute, Harvard T.H. Chan School of Public HealthCenter for Functional Cancer Epigenetics, Dana-Farber Cancer InstituteDepartment of Biostatistics and Bioinformatics, Roswell Park Comprehensive Cancer CenterDepartment of Data Science, Dana-Farber Cancer Institute, Harvard T.H. Chan School of Public HealthAbstract We present Model-based AnalysEs of Transcriptome and RegulOme (MAESTRO), a comprehensive open-source computational workflow ( http://github.com/liulab-dfci/MAESTRO ) for the integrative analyses of single-cell RNA-seq (scRNA-seq) and ATAC-seq (scATAC-seq) data from multiple platforms. MAESTRO provides functions for pre-processing, alignment, quality control, expression and chromatin accessibility quantification, clustering, differential analysis, and annotation. By modeling gene regulatory potential from chromatin accessibilities at the single-cell level, MAESTRO outperforms the existing methods for integrating the cell clusters between scRNA-seq and scATAC-seq. Furthermore, MAESTRO supports automatic cell-type annotation using predefined cell type marker genes and identifies driver regulators from differential scRNA-seq genes and scATAC-seq peaks.http://link.springer.com/article/10.1186/s13059-020-02116-xSingle-cell RNA-seqSingle-cell ATAC-seqComputational workflowIntegrate scRNA-seq and scATAC-seqCell-type annotationPredict transcriptional regulators |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chenfei Wang Dongqing Sun Xin Huang Changxin Wan Ziyi Li Ya Han Qian Qin Jingyu Fan Xintao Qiu Yingtian Xie Clifford A. Meyer Myles Brown Ming Tang Henry Long Tao Liu X. Shirley Liu |
spellingShingle |
Chenfei Wang Dongqing Sun Xin Huang Changxin Wan Ziyi Li Ya Han Qian Qin Jingyu Fan Xintao Qiu Yingtian Xie Clifford A. Meyer Myles Brown Ming Tang Henry Long Tao Liu X. Shirley Liu Integrative analyses of single-cell transcriptome and regulome using MAESTRO Genome Biology Single-cell RNA-seq Single-cell ATAC-seq Computational workflow Integrate scRNA-seq and scATAC-seq Cell-type annotation Predict transcriptional regulators |
author_facet |
Chenfei Wang Dongqing Sun Xin Huang Changxin Wan Ziyi Li Ya Han Qian Qin Jingyu Fan Xintao Qiu Yingtian Xie Clifford A. Meyer Myles Brown Ming Tang Henry Long Tao Liu X. Shirley Liu |
author_sort |
Chenfei Wang |
title |
Integrative analyses of single-cell transcriptome and regulome using MAESTRO |
title_short |
Integrative analyses of single-cell transcriptome and regulome using MAESTRO |
title_full |
Integrative analyses of single-cell transcriptome and regulome using MAESTRO |
title_fullStr |
Integrative analyses of single-cell transcriptome and regulome using MAESTRO |
title_full_unstemmed |
Integrative analyses of single-cell transcriptome and regulome using MAESTRO |
title_sort |
integrative analyses of single-cell transcriptome and regulome using maestro |
publisher |
BMC |
series |
Genome Biology |
issn |
1474-760X |
publishDate |
2020-08-01 |
description |
Abstract We present Model-based AnalysEs of Transcriptome and RegulOme (MAESTRO), a comprehensive open-source computational workflow ( http://github.com/liulab-dfci/MAESTRO ) for the integrative analyses of single-cell RNA-seq (scRNA-seq) and ATAC-seq (scATAC-seq) data from multiple platforms. MAESTRO provides functions for pre-processing, alignment, quality control, expression and chromatin accessibility quantification, clustering, differential analysis, and annotation. By modeling gene regulatory potential from chromatin accessibilities at the single-cell level, MAESTRO outperforms the existing methods for integrating the cell clusters between scRNA-seq and scATAC-seq. Furthermore, MAESTRO supports automatic cell-type annotation using predefined cell type marker genes and identifies driver regulators from differential scRNA-seq genes and scATAC-seq peaks. |
topic |
Single-cell RNA-seq Single-cell ATAC-seq Computational workflow Integrate scRNA-seq and scATAC-seq Cell-type annotation Predict transcriptional regulators |
url |
http://link.springer.com/article/10.1186/s13059-020-02116-x |
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