Cross-Sectional Study on the Gut Microbiome of Parkinson’s Disease Patients in Central China
Gastrointestinal dysfunction plays an important role in the occurrence and development of Parkinson’s disease (PD). This study investigates the composition of the gut microbiome using shotgun metagenomic sequencing in PD patients in central China. Fecal samples from 39 PD patients (PD group) and the...
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doaj-a14ed6d8125c4b0493f83fda19877b4a2021-09-28T06:48:44ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-09-011210.3389/fmicb.2021.728479728479Cross-Sectional Study on the Gut Microbiome of Parkinson’s Disease Patients in Central ChinaLiangwei Mao0Yu Zhang1Jing Tian2Ming Sang3Guimin Zhang4Yuling Zhou5Puqing Wang6State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Biological Resources, School of Life Sciences, Hubei University, Wuhan, ChinaHubei Clinical Research Center of Parkinson’s Disease, Xiangyang No. 1 People’s Hospital, Hubei University of Medicine, Xiangyang, ChinaHubei Clinical Research Center of Parkinson’s Disease, Xiangyang No. 1 People’s Hospital, Hubei University of Medicine, Xiangyang, ChinaHubei Clinical Research Center of Parkinson’s Disease, Xiangyang No. 1 People’s Hospital, Hubei University of Medicine, Xiangyang, ChinaState Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Biological Resources, School of Life Sciences, Hubei University, Wuhan, ChinaState Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Biological Resources, School of Life Sciences, Hubei University, Wuhan, ChinaHubei Clinical Research Center of Parkinson’s Disease, Xiangyang No. 1 People’s Hospital, Hubei University of Medicine, Xiangyang, ChinaGastrointestinal dysfunction plays an important role in the occurrence and development of Parkinson’s disease (PD). This study investigates the composition of the gut microbiome using shotgun metagenomic sequencing in PD patients in central China. Fecal samples from 39 PD patients (PD group) and the corresponding 39 healthy spouses of the patients (SP) were collected for shotgun metagenomics sequencing. Results showed a significantly altered microbial composition in the PD patients. Bilophila wadsworthia enrichment was found in the gut microbiome of PD patients, which has not been reported in previous studies. The random forest (RF) model, which identifies differences in microbiomes, reliably discriminated patients with PD from controls; the area under the receiver operating characteristic curve was 0.803. Further analysis of the microbiome and clinical symptoms showed that Klebsiella and Parasutterella were positively correlated with the duration and severity of PD, whereas hydrogen-generating Prevotella was negatively correlated with disease severity. The Cluster of Orthologous Groups of protein database, the KEGG Orthology database, and the carbohydrate-active enzymes of gene-category analysis showed that branched-chain amino acid–related proteins were significantly increased, and GH43 was significantly reduced in the PD group. Functional analysis of the metagenome confirmed differences in microbiome metabolism in the PD group related to short-chain fatty acid precursor metabolism.https://www.frontiersin.org/articles/10.3389/fmicb.2021.728479/fullParkinson’s diseasegut-brain-axisshotgun metagenomic sequencinggastrointestinal dysbiosisshort-chain fatty acids |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Liangwei Mao Yu Zhang Jing Tian Ming Sang Guimin Zhang Yuling Zhou Puqing Wang |
spellingShingle |
Liangwei Mao Yu Zhang Jing Tian Ming Sang Guimin Zhang Yuling Zhou Puqing Wang Cross-Sectional Study on the Gut Microbiome of Parkinson’s Disease Patients in Central China Frontiers in Microbiology Parkinson’s disease gut-brain-axis shotgun metagenomic sequencing gastrointestinal dysbiosis short-chain fatty acids |
author_facet |
Liangwei Mao Yu Zhang Jing Tian Ming Sang Guimin Zhang Yuling Zhou Puqing Wang |
author_sort |
Liangwei Mao |
title |
Cross-Sectional Study on the Gut Microbiome of Parkinson’s Disease Patients in Central China |
title_short |
Cross-Sectional Study on the Gut Microbiome of Parkinson’s Disease Patients in Central China |
title_full |
Cross-Sectional Study on the Gut Microbiome of Parkinson’s Disease Patients in Central China |
title_fullStr |
Cross-Sectional Study on the Gut Microbiome of Parkinson’s Disease Patients in Central China |
title_full_unstemmed |
Cross-Sectional Study on the Gut Microbiome of Parkinson’s Disease Patients in Central China |
title_sort |
cross-sectional study on the gut microbiome of parkinson’s disease patients in central china |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2021-09-01 |
description |
Gastrointestinal dysfunction plays an important role in the occurrence and development of Parkinson’s disease (PD). This study investigates the composition of the gut microbiome using shotgun metagenomic sequencing in PD patients in central China. Fecal samples from 39 PD patients (PD group) and the corresponding 39 healthy spouses of the patients (SP) were collected for shotgun metagenomics sequencing. Results showed a significantly altered microbial composition in the PD patients. Bilophila wadsworthia enrichment was found in the gut microbiome of PD patients, which has not been reported in previous studies. The random forest (RF) model, which identifies differences in microbiomes, reliably discriminated patients with PD from controls; the area under the receiver operating characteristic curve was 0.803. Further analysis of the microbiome and clinical symptoms showed that Klebsiella and Parasutterella were positively correlated with the duration and severity of PD, whereas hydrogen-generating Prevotella was negatively correlated with disease severity. The Cluster of Orthologous Groups of protein database, the KEGG Orthology database, and the carbohydrate-active enzymes of gene-category analysis showed that branched-chain amino acid–related proteins were significantly increased, and GH43 was significantly reduced in the PD group. Functional analysis of the metagenome confirmed differences in microbiome metabolism in the PD group related to short-chain fatty acid precursor metabolism. |
topic |
Parkinson’s disease gut-brain-axis shotgun metagenomic sequencing gastrointestinal dysbiosis short-chain fatty acids |
url |
https://www.frontiersin.org/articles/10.3389/fmicb.2021.728479/full |
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