Hepatoprotective effects of lycopene against carbon tetrachloride-induced acute liver injury in rats
Lycopene, the major carotenoid in tomatoes, is a known antioxidant that may lower oxidative stress biomarkers by a mechanism that is not fully elucidated. The intoxication of rats with carbon tetrachloride (CCl4) resulted in significant histological hepatic degradation accompanied by a marked increa...
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doaj-a15b541910024d099564d6f6bcf05a9a2021-04-29T04:41:26ZengElsevierJournal of Functional Foods1756-46462013-10-015416011610Hepatoprotective effects of lycopene against carbon tetrachloride-induced acute liver injury in ratsCarmen Pinto0Beatriz Rodríguez-Galdón1Juan J. Cestero2Pedro Macías3Department of Biochemistry and Molecular Biology, Science Faculty, Extremadura University, Av. Elvas s/n, 06006 Badajoz, SpainDepartment of Biochemistry and Molecular Biology, Science Faculty, Extremadura University, Av. Elvas s/n, 06006 Badajoz, SpainDepartment of Biochemistry and Molecular Biology, Science Faculty, Extremadura University, Av. Elvas s/n, 06006 Badajoz, SpainCorresponding author. Tel.: +34 924389419.; Department of Biochemistry and Molecular Biology, Science Faculty, Extremadura University, Av. Elvas s/n, 06006 Badajoz, SpainLycopene, the major carotenoid in tomatoes, is a known antioxidant that may lower oxidative stress biomarkers by a mechanism that is not fully elucidated. The intoxication of rats with carbon tetrachloride (CCl4) resulted in significant histological hepatic degradation accompanied by a marked increase in reactive oxygen species (ROS) and in the number of apoptotic cells. The induced oxidative stress in turn results in a significant elevation of lipid peroxidation and H2O2 generation, together with a decrease in the concentration of reduced glutathione (GSH) and a significant reduction in activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S transferase (GST). CCl4-intoxicated rats, pre-treated with lycopene, showed strongly reduced cell damage and ROS generation. The level of markers for hepatic integrity in lycopene pre-treated rats was close to the controls in the absence of CCl4 treatment, indicating the protective effect of lycopene pre-treatment. In the same way, lycopene pre-treated rats significantly increased SOD, CAT, GPx, GST activities and GSH level. In addition, we measured an increased lipoxygenase (LOX) activity in CCl4-intoxicated rats. This activity was reduced in lycopene pre-treated rats to values close to those observed in the controls, suggesting a potential pharmacological application of this dietary carotenoid.http://www.sciencedirect.com/science/article/pii/S1756464613001503LycopeneLiverAntioxidantsStress oxidative |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Carmen Pinto Beatriz Rodríguez-Galdón Juan J. Cestero Pedro Macías |
spellingShingle |
Carmen Pinto Beatriz Rodríguez-Galdón Juan J. Cestero Pedro Macías Hepatoprotective effects of lycopene against carbon tetrachloride-induced acute liver injury in rats Journal of Functional Foods Lycopene Liver Antioxidants Stress oxidative |
author_facet |
Carmen Pinto Beatriz Rodríguez-Galdón Juan J. Cestero Pedro Macías |
author_sort |
Carmen Pinto |
title |
Hepatoprotective effects of lycopene against carbon tetrachloride-induced acute liver injury in rats |
title_short |
Hepatoprotective effects of lycopene against carbon tetrachloride-induced acute liver injury in rats |
title_full |
Hepatoprotective effects of lycopene against carbon tetrachloride-induced acute liver injury in rats |
title_fullStr |
Hepatoprotective effects of lycopene against carbon tetrachloride-induced acute liver injury in rats |
title_full_unstemmed |
Hepatoprotective effects of lycopene against carbon tetrachloride-induced acute liver injury in rats |
title_sort |
hepatoprotective effects of lycopene against carbon tetrachloride-induced acute liver injury in rats |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2013-10-01 |
description |
Lycopene, the major carotenoid in tomatoes, is a known antioxidant that may lower oxidative stress biomarkers by a mechanism that is not fully elucidated. The intoxication of rats with carbon tetrachloride (CCl4) resulted in significant histological hepatic degradation accompanied by a marked increase in reactive oxygen species (ROS) and in the number of apoptotic cells. The induced oxidative stress in turn results in a significant elevation of lipid peroxidation and H2O2 generation, together with a decrease in the concentration of reduced glutathione (GSH) and a significant reduction in activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S transferase (GST). CCl4-intoxicated rats, pre-treated with lycopene, showed strongly reduced cell damage and ROS generation. The level of markers for hepatic integrity in lycopene pre-treated rats was close to the controls in the absence of CCl4 treatment, indicating the protective effect of lycopene pre-treatment. In the same way, lycopene pre-treated rats significantly increased SOD, CAT, GPx, GST activities and GSH level. In addition, we measured an increased lipoxygenase (LOX) activity in CCl4-intoxicated rats. This activity was reduced in lycopene pre-treated rats to values close to those observed in the controls, suggesting a potential pharmacological application of this dietary carotenoid. |
topic |
Lycopene Liver Antioxidants Stress oxidative |
url |
http://www.sciencedirect.com/science/article/pii/S1756464613001503 |
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