Planar Lymphoscintigraphy for sentinel lymph node mapping in dogs with mast cell tumor: a pilot study.
The histopathological assessment of the first node receiving lymphatic drainage from a tumor – defined as Sentinel Lymph Node (SLN) – is essential to determine stage, therapy and outcome in oncological patients. Both in human and veterinary medicine, lymphoscintigraphy is a recognized procedure for...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Università degli Studi di Milano
2018-06-01
|
Series: | International Journal of Health, Animal Science and Food Safety |
Subjects: | |
Online Access: | https://riviste.unimi.it/index.php/haf/article/view/10022 |
id |
doaj-a16acab3596a4603b27ca85606ac8152 |
---|---|
record_format |
Article |
spelling |
doaj-a16acab3596a4603b27ca85606ac81522020-11-25T03:18:57ZengUniversità degli Studi di MilanoInternational Journal of Health, Animal Science and Food Safety2283-39272018-06-0151s10.13130/2283-3927/100228741Planar Lymphoscintigraphy for sentinel lymph node mapping in dogs with mast cell tumor: a pilot study.Martina Manfredi0Donatella De Zani1Davide Danilo Zani2Department of Veterinary Medicine, Università degli Studi di Milano, Via Celoria 10, 20133 Milan, ItalyDepartment of Veterinary Medicine, Università degli Studi di Milano, Via Celoria 10, 20133 Milan, ItalyDepartment of Veterinary Medicine, Università degli Studi di Milano, Via Celoria 10, 20133 Milan, ItalyThe histopathological assessment of the first node receiving lymphatic drainage from a tumor – defined as Sentinel Lymph Node (SLN) – is essential to determine stage, therapy and outcome in oncological patients. Both in human and veterinary medicine, lymphoscintigraphy is a recognized procedure for SLN detection (Mariani et al., 2004; Tuohy et al., 2009; Beer et al., 2017). In this study, we want to determine the most suitable pre-operative planar lymphoscintigraphy protocol for SLN mapping in dogs with mast cell tumor (MCT). We selected 5 dogs diagnosed with cutaneous MTC, with clinically negative lymph nodes and no distant metastasis, undergoing surgical tumor removal, and we obtained owner’s written consent. Planar lymphoscintigraphy was performed in patients under general anesthesia, after subcutaneous peritumoral injection of different doses of technetium-99m (Tc-99m) labelled colloid diluted reaching a 0.5 ml volume (Worley, 2014). The MegaBecquerel value (MBq) of the syringe was measured before and after the injection. Dynamic images (1 frame/second for 60 seconds) were taken at the moment of the injection, 3 and 8 minutes after the injection. Ventrodorsal (VD) and lateral (L) static images (120 seconds/frame) were taken until the identification of SLN had been made. If needed, the injection site was masked with a 2-mm lead foil. Results are showed in Table 1. In patient 1, the SLN was not identify, probably due to a superimposition with the injection site. During the study, we increased the injected MBq dose, in order to better visualize lymphatic path and SLN (Balogh et al., 2002). In fact, the number of static images needed to identify SLN have been reduced from 8,7 to 6. Masking the injection site proved to be useful for a better visualization of SLN. Dynamic images showed to be unnecessary for the SLN identification. For further studies, we suggest the injection of minimum 23,5 MBq Tc-99m activity and the acquisition of VD and L static images with and without masking the injection site.https://riviste.unimi.it/index.php/haf/article/view/10022Sentinel Lymph NodeMast Cell TumorLymphoscintigraphyDiagnostic ImagingOncology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Martina Manfredi Donatella De Zani Davide Danilo Zani |
spellingShingle |
Martina Manfredi Donatella De Zani Davide Danilo Zani Planar Lymphoscintigraphy for sentinel lymph node mapping in dogs with mast cell tumor: a pilot study. International Journal of Health, Animal Science and Food Safety Sentinel Lymph Node Mast Cell Tumor Lymphoscintigraphy Diagnostic Imaging Oncology |
author_facet |
Martina Manfredi Donatella De Zani Davide Danilo Zani |
author_sort |
Martina Manfredi |
title |
Planar Lymphoscintigraphy for sentinel lymph node mapping in dogs with mast cell tumor: a pilot study. |
title_short |
Planar Lymphoscintigraphy for sentinel lymph node mapping in dogs with mast cell tumor: a pilot study. |
title_full |
Planar Lymphoscintigraphy for sentinel lymph node mapping in dogs with mast cell tumor: a pilot study. |
title_fullStr |
Planar Lymphoscintigraphy for sentinel lymph node mapping in dogs with mast cell tumor: a pilot study. |
title_full_unstemmed |
Planar Lymphoscintigraphy for sentinel lymph node mapping in dogs with mast cell tumor: a pilot study. |
title_sort |
planar lymphoscintigraphy for sentinel lymph node mapping in dogs with mast cell tumor: a pilot study. |
publisher |
Università degli Studi di Milano |
series |
International Journal of Health, Animal Science and Food Safety |
issn |
2283-3927 |
publishDate |
2018-06-01 |
description |
The histopathological assessment of the first node receiving lymphatic drainage from a tumor – defined as Sentinel Lymph Node (SLN) – is essential to determine stage, therapy and outcome in oncological patients. Both in human and veterinary medicine, lymphoscintigraphy is a recognized procedure for SLN detection (Mariani et al., 2004; Tuohy et al., 2009; Beer et al., 2017). In this study, we want to determine the most suitable pre-operative planar lymphoscintigraphy protocol for SLN mapping in dogs with mast cell tumor (MCT). We selected 5 dogs diagnosed with cutaneous MTC, with clinically negative lymph nodes and no distant metastasis, undergoing surgical tumor removal, and we obtained owner’s written consent. Planar lymphoscintigraphy was performed in patients under general anesthesia, after subcutaneous peritumoral injection of different doses of technetium-99m (Tc-99m) labelled colloid diluted reaching a 0.5 ml volume (Worley, 2014). The MegaBecquerel value (MBq) of the syringe was measured before and after the injection. Dynamic images (1 frame/second for 60 seconds) were taken at the moment of the injection, 3 and 8 minutes after the injection. Ventrodorsal (VD) and lateral (L) static images (120 seconds/frame) were taken until the identification of SLN had been made. If needed, the injection site was masked with a 2-mm lead foil. Results are showed in Table 1. In patient 1, the SLN was not identify, probably due to a superimposition with the injection site. During the study, we increased the injected MBq dose, in order to better visualize lymphatic path and SLN (Balogh et al., 2002). In fact, the number of static images needed to identify SLN have been reduced from 8,7 to 6. Masking the injection site proved to be useful for a better visualization of SLN. Dynamic images showed to be unnecessary for the SLN identification. For further studies, we suggest the injection of minimum 23,5 MBq Tc-99m activity and the acquisition of VD and L static images with and without masking the injection site. |
topic |
Sentinel Lymph Node Mast Cell Tumor Lymphoscintigraphy Diagnostic Imaging Oncology |
url |
https://riviste.unimi.it/index.php/haf/article/view/10022 |
work_keys_str_mv |
AT martinamanfredi planarlymphoscintigraphyforsentinellymphnodemappingindogswithmastcelltumorapilotstudy AT donatelladezani planarlymphoscintigraphyforsentinellymphnodemappingindogswithmastcelltumorapilotstudy AT davidedanilozani planarlymphoscintigraphyforsentinellymphnodemappingindogswithmastcelltumorapilotstudy |
_version_ |
1724624718397440000 |