Impact of early chimerism status on clinical outcome in children with acute lymphoblastic leukaemia after haematopoietic stem cell transplantation
Abstract Background The significance of very early chimerism assessment before day + 28, which is considered the moment of engraftment, is still unclear. In this retrospective study, we evaluated the clinical impact of very early chimerism on the clinical outcome after allogeneic haematopoietic stem...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2019-11-01
|
Series: | BMC Cancer |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12885-019-6360-3 |
id |
doaj-a1745341d37d4cab96355e948cb36e74 |
---|---|
record_format |
Article |
spelling |
doaj-a1745341d37d4cab96355e948cb36e742020-11-25T01:32:28ZengBMCBMC Cancer1471-24072019-11-011911810.1186/s12885-019-6360-3Impact of early chimerism status on clinical outcome in children with acute lymphoblastic leukaemia after haematopoietic stem cell transplantationMonika Lejman0Agnieszka Zaucha-Prażmo1Joanna Zawitkowska2Aleksandra Mroczkowska3Dominik Grabowski4Jerzy R. Kowalczyk5Katarzyna Drabko6Laboratory of Genetic Diagnostics, Department of Pediatric Hematology, Oncology, and Transplantology, Medical University of LublinDepartment of Pediatric Hematology, Oncology, and Transplantology, Medical University of LublinDepartment of Pediatric Hematology, Oncology, and Transplantology, Medical University of LublinLaboratory of Genetic Diagnostics, Department of Pediatric Hematology, Oncology, and Transplantology, Medical University of LublinDepartment of Pediatric Hematology, Oncology, and Transplantology, Medical University of LublinDepartment of Pediatric Hematology, Oncology, and Transplantology, Medical University of LublinDepartment of Pediatric Hematology, Oncology, and Transplantology, Medical University of LublinAbstract Background The significance of very early chimerism assessment before day + 28, which is considered the moment of engraftment, is still unclear. In this retrospective study, we evaluated the clinical impact of very early chimerism on the clinical outcome after allogeneic haematopoietic stem cell transplantation (allo-HSCT) in children with acute lymphoblastic leukaemia (ALL). Methods The study group included 38 boys and 18 girls. Very early chimerism was evaluated on days + 7, + 14, + 21 and + 28 after the transplant. Short tandem repeat polymerase chain reaction (STR PCR) was used to analyse chimerism. Results Overall survival (OS) and event-free survival (EFS) were 84 and 80%, respectively. The OS in the group of 24 patients with complete donor chimerism on day + 14 was 83%, and it did not differ statistically compared to the 32 patients with mixed chimerism on day + 14 (OS was 84%). In our cohort of patients, the matched unrelated donor, male gender of donor, number of transplanted cells above 4.47 × 106 kg and no serotherapy with anti-thymocyte globulin (ATG) were statistically related to a higher level of donor chimerism. The immunophenotypes of disease, age of patient at time HSCT, recipient sex, stem cell source (peripheral blood/bone marrow) and conditioning regimen had no impact on early chimerism. Acute graft versus host disease grades II-IV was diagnosed in 23 patients who presented with donor chimerism levels above 60% on day 7. Conclusions The data presented in this study provide valuable insight into the analysis of very early chimerism in children with ALL treated with HSCT.http://link.springer.com/article/10.1186/s12885-019-6360-3ChimerismEngraftmentQuantitative PCRGvHDAcute lymphoblastic leukaemiaAllogeneic Haematopoietic stem cell transplantation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Monika Lejman Agnieszka Zaucha-Prażmo Joanna Zawitkowska Aleksandra Mroczkowska Dominik Grabowski Jerzy R. Kowalczyk Katarzyna Drabko |
spellingShingle |
Monika Lejman Agnieszka Zaucha-Prażmo Joanna Zawitkowska Aleksandra Mroczkowska Dominik Grabowski Jerzy R. Kowalczyk Katarzyna Drabko Impact of early chimerism status on clinical outcome in children with acute lymphoblastic leukaemia after haematopoietic stem cell transplantation BMC Cancer Chimerism Engraftment Quantitative PCR GvHD Acute lymphoblastic leukaemia Allogeneic Haematopoietic stem cell transplantation |
author_facet |
Monika Lejman Agnieszka Zaucha-Prażmo Joanna Zawitkowska Aleksandra Mroczkowska Dominik Grabowski Jerzy R. Kowalczyk Katarzyna Drabko |
author_sort |
Monika Lejman |
title |
Impact of early chimerism status on clinical outcome in children with acute lymphoblastic leukaemia after haematopoietic stem cell transplantation |
title_short |
Impact of early chimerism status on clinical outcome in children with acute lymphoblastic leukaemia after haematopoietic stem cell transplantation |
title_full |
Impact of early chimerism status on clinical outcome in children with acute lymphoblastic leukaemia after haematopoietic stem cell transplantation |
title_fullStr |
Impact of early chimerism status on clinical outcome in children with acute lymphoblastic leukaemia after haematopoietic stem cell transplantation |
title_full_unstemmed |
Impact of early chimerism status on clinical outcome in children with acute lymphoblastic leukaemia after haematopoietic stem cell transplantation |
title_sort |
impact of early chimerism status on clinical outcome in children with acute lymphoblastic leukaemia after haematopoietic stem cell transplantation |
publisher |
BMC |
series |
BMC Cancer |
issn |
1471-2407 |
publishDate |
2019-11-01 |
description |
Abstract Background The significance of very early chimerism assessment before day + 28, which is considered the moment of engraftment, is still unclear. In this retrospective study, we evaluated the clinical impact of very early chimerism on the clinical outcome after allogeneic haematopoietic stem cell transplantation (allo-HSCT) in children with acute lymphoblastic leukaemia (ALL). Methods The study group included 38 boys and 18 girls. Very early chimerism was evaluated on days + 7, + 14, + 21 and + 28 after the transplant. Short tandem repeat polymerase chain reaction (STR PCR) was used to analyse chimerism. Results Overall survival (OS) and event-free survival (EFS) were 84 and 80%, respectively. The OS in the group of 24 patients with complete donor chimerism on day + 14 was 83%, and it did not differ statistically compared to the 32 patients with mixed chimerism on day + 14 (OS was 84%). In our cohort of patients, the matched unrelated donor, male gender of donor, number of transplanted cells above 4.47 × 106 kg and no serotherapy with anti-thymocyte globulin (ATG) were statistically related to a higher level of donor chimerism. The immunophenotypes of disease, age of patient at time HSCT, recipient sex, stem cell source (peripheral blood/bone marrow) and conditioning regimen had no impact on early chimerism. Acute graft versus host disease grades II-IV was diagnosed in 23 patients who presented with donor chimerism levels above 60% on day 7. Conclusions The data presented in this study provide valuable insight into the analysis of very early chimerism in children with ALL treated with HSCT. |
topic |
Chimerism Engraftment Quantitative PCR GvHD Acute lymphoblastic leukaemia Allogeneic Haematopoietic stem cell transplantation |
url |
http://link.springer.com/article/10.1186/s12885-019-6360-3 |
work_keys_str_mv |
AT monikalejman impactofearlychimerismstatusonclinicaloutcomeinchildrenwithacutelymphoblasticleukaemiaafterhaematopoieticstemcelltransplantation AT agnieszkazauchaprazmo impactofearlychimerismstatusonclinicaloutcomeinchildrenwithacutelymphoblasticleukaemiaafterhaematopoieticstemcelltransplantation AT joannazawitkowska impactofearlychimerismstatusonclinicaloutcomeinchildrenwithacutelymphoblasticleukaemiaafterhaematopoieticstemcelltransplantation AT aleksandramroczkowska impactofearlychimerismstatusonclinicaloutcomeinchildrenwithacutelymphoblasticleukaemiaafterhaematopoieticstemcelltransplantation AT dominikgrabowski impactofearlychimerismstatusonclinicaloutcomeinchildrenwithacutelymphoblasticleukaemiaafterhaematopoieticstemcelltransplantation AT jerzyrkowalczyk impactofearlychimerismstatusonclinicaloutcomeinchildrenwithacutelymphoblasticleukaemiaafterhaematopoieticstemcelltransplantation AT katarzynadrabko impactofearlychimerismstatusonclinicaloutcomeinchildrenwithacutelymphoblasticleukaemiaafterhaematopoieticstemcelltransplantation |
_version_ |
1725081961498673152 |