Single-Cell Analysis Reveals Characterization of Infiltrating T Cells in Moderately Differentiated Colorectal Cancer
ObjectiveThis study aimed to characterize the tumor-infiltrating T cells in moderately differentiated colorectal cancer.MethodsUsing single-cell RNA sequencing data of isolated 1632 T cells from tumor tissue and 1252 T cells from the peripheral blood of CRC patients, unsupervised clustering analysis...
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doaj-a18235b531eb4b1780a8898f37a3346a2021-02-18T13:53:29ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-01-011110.3389/fimmu.2020.620196620196Single-Cell Analysis Reveals Characterization of Infiltrating T Cells in Moderately Differentiated Colorectal CancerXi Yang0Quan Qi1Yuefen Pan2Qing Zhou3Yinhang Wu4Jing Zhuang5Jiamin Xu6Mingyue Pan7Shuwen Han8Department of Oncology, Huzhou Cent Hospital, Affiliated Cent Hospital HuZhou University, Huzhou, ChinaDepartment of Oncology, Huzhou Cent Hospital, Affiliated Cent Hospital HuZhou University, Huzhou, ChinaDepartment of Oncology, Huzhou Cent Hospital, Affiliated Cent Hospital HuZhou University, Huzhou, ChinaDepartment of Critical Care Medicine, Huzhou Cent Hospital, Affiliated Cent Hospital HuZhou University, Huzhou, ChinaGraduate School of Second Clinical Medicine Faculty, Zhejiang Chinese Medical University, Hangzhou, ChinaGraduate School of Nursing, Huzhou University, Huzhou, ChinaGraduate School of Nursing, Huzhou University, Huzhou, ChinaGraduate School of Nursing, Huzhou University, Huzhou, ChinaDepartment of Oncology, Huzhou Cent Hospital, Affiliated Cent Hospital HuZhou University, Huzhou, ChinaObjectiveThis study aimed to characterize the tumor-infiltrating T cells in moderately differentiated colorectal cancer.MethodsUsing single-cell RNA sequencing data of isolated 1632 T cells from tumor tissue and 1252 T cells from the peripheral blood of CRC patients, unsupervised clustering analysis was performed to identify functionally distinct T cell populations, followed by correlations and ligand-receptor interactions across cell types. Finally, differential analysis of the tumor-infiltrating T cells between colon cancer and rectal cancer were carried out.ResultsA total of eight distinct T cell populations were identified from tumor tissue. Tumor-Treg showed a strong correlation with Th17 cells. CD8+TRM was positively correlated with CD8+IEL. Seven distinct T cell populations were identified from peripheral blood. There was a strong correlation between CD4+TN and CD4+blood-TCM. Colon cancer and rectal cancer showed differences in the composition of tumor-infiltrating T cell populations. Tumor-infiltrating CD8+IEL cells were found in rectal cancer but not in colon cancer, while CD8+ TN cells were found in the peripheral blood of colon cancer but not in that of rectal cancer. A larger number of tumor-infiltrating CD8+ Tex (88.94%) cells were found in the colon cancer than in the rectal cancer (11.06%). The T cells of the colon and rectal cancers showed changes in gene expression pattern.ConclusionsWe characterized the T cell populations in the CRC tumor tissue and peripheral blood.https://www.frontiersin.org/articles/10.3389/fimmu.2020.620196/fullcolon cancerrectal cancersingle-cell RNA sequencingtumor-infiltrating T cellsimmunotherapy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xi Yang Quan Qi Yuefen Pan Qing Zhou Yinhang Wu Jing Zhuang Jiamin Xu Mingyue Pan Shuwen Han |
spellingShingle |
Xi Yang Quan Qi Yuefen Pan Qing Zhou Yinhang Wu Jing Zhuang Jiamin Xu Mingyue Pan Shuwen Han Single-Cell Analysis Reveals Characterization of Infiltrating T Cells in Moderately Differentiated Colorectal Cancer Frontiers in Immunology colon cancer rectal cancer single-cell RNA sequencing tumor-infiltrating T cells immunotherapy |
author_facet |
Xi Yang Quan Qi Yuefen Pan Qing Zhou Yinhang Wu Jing Zhuang Jiamin Xu Mingyue Pan Shuwen Han |
author_sort |
Xi Yang |
title |
Single-Cell Analysis Reveals Characterization of Infiltrating T Cells in Moderately Differentiated Colorectal Cancer |
title_short |
Single-Cell Analysis Reveals Characterization of Infiltrating T Cells in Moderately Differentiated Colorectal Cancer |
title_full |
Single-Cell Analysis Reveals Characterization of Infiltrating T Cells in Moderately Differentiated Colorectal Cancer |
title_fullStr |
Single-Cell Analysis Reveals Characterization of Infiltrating T Cells in Moderately Differentiated Colorectal Cancer |
title_full_unstemmed |
Single-Cell Analysis Reveals Characterization of Infiltrating T Cells in Moderately Differentiated Colorectal Cancer |
title_sort |
single-cell analysis reveals characterization of infiltrating t cells in moderately differentiated colorectal cancer |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2021-01-01 |
description |
ObjectiveThis study aimed to characterize the tumor-infiltrating T cells in moderately differentiated colorectal cancer.MethodsUsing single-cell RNA sequencing data of isolated 1632 T cells from tumor tissue and 1252 T cells from the peripheral blood of CRC patients, unsupervised clustering analysis was performed to identify functionally distinct T cell populations, followed by correlations and ligand-receptor interactions across cell types. Finally, differential analysis of the tumor-infiltrating T cells between colon cancer and rectal cancer were carried out.ResultsA total of eight distinct T cell populations were identified from tumor tissue. Tumor-Treg showed a strong correlation with Th17 cells. CD8+TRM was positively correlated with CD8+IEL. Seven distinct T cell populations were identified from peripheral blood. There was a strong correlation between CD4+TN and CD4+blood-TCM. Colon cancer and rectal cancer showed differences in the composition of tumor-infiltrating T cell populations. Tumor-infiltrating CD8+IEL cells were found in rectal cancer but not in colon cancer, while CD8+ TN cells were found in the peripheral blood of colon cancer but not in that of rectal cancer. A larger number of tumor-infiltrating CD8+ Tex (88.94%) cells were found in the colon cancer than in the rectal cancer (11.06%). The T cells of the colon and rectal cancers showed changes in gene expression pattern.ConclusionsWe characterized the T cell populations in the CRC tumor tissue and peripheral blood. |
topic |
colon cancer rectal cancer single-cell RNA sequencing tumor-infiltrating T cells immunotherapy |
url |
https://www.frontiersin.org/articles/10.3389/fimmu.2020.620196/full |
work_keys_str_mv |
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