High sPLA2-IIA level is associated with eicosanoid metabolism in patients with bacterial sepsis syndrome.
The aim of this study was to determine the association between secretory phospholipase A2 group IIA (sPLA2-IIA) and eicosanoid pathway metabolites in patients with bacterial sepsis syndrome (BSS). Levels of sPLA2-IIA, eicosanoids prostaglandin (PG)E2, PGD synthase were quantified in the sera from pa...
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doaj-a1915c703a8245f3b94deb832d77cbbb2021-03-03T21:36:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01153e023028510.1371/journal.pone.0230285High sPLA2-IIA level is associated with eicosanoid metabolism in patients with bacterial sepsis syndrome.Nurul Saadah AhmadToh Leong TanKhaizurin Tajul ArifinWan Zurinah Wan NgahYasmin Anum Mohd YusofThe aim of this study was to determine the association between secretory phospholipase A2 group IIA (sPLA2-IIA) and eicosanoid pathway metabolites in patients with bacterial sepsis syndrome (BSS). Levels of sPLA2-IIA, eicosanoids prostaglandin (PG)E2, PGD synthase were quantified in the sera from patients confirmed to have bacterial sepsis (BS; N = 45), bacterial severe sepsis/septic shock (BSS/SS; N = 35) and healthy subjects (N = 45). Cyclooxygenase (COX)-1 and COX-2 activities were analyzed from cell lysate. Serum levels of sPLA2-IIA, PGE2, and PGDS increased significantly in patients with BS and BSS/SS compared to healthy subjects (p<0.05). COX-2 activity was significantly increased in patients with BS compared to healthy subjects (p<0.05), but not COX-1 activity. Binary logistic regression analysis showed that sPLA2-IIA and PGE2 were independent factors predicting BSS severity. In conclusion, high level of sPLA2-IIA is associated with eicosanoid metabolism in patients with BSS.https://doi.org/10.1371/journal.pone.0230285 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nurul Saadah Ahmad Toh Leong Tan Khaizurin Tajul Arifin Wan Zurinah Wan Ngah Yasmin Anum Mohd Yusof |
spellingShingle |
Nurul Saadah Ahmad Toh Leong Tan Khaizurin Tajul Arifin Wan Zurinah Wan Ngah Yasmin Anum Mohd Yusof High sPLA2-IIA level is associated with eicosanoid metabolism in patients with bacterial sepsis syndrome. PLoS ONE |
author_facet |
Nurul Saadah Ahmad Toh Leong Tan Khaizurin Tajul Arifin Wan Zurinah Wan Ngah Yasmin Anum Mohd Yusof |
author_sort |
Nurul Saadah Ahmad |
title |
High sPLA2-IIA level is associated with eicosanoid metabolism in patients with bacterial sepsis syndrome. |
title_short |
High sPLA2-IIA level is associated with eicosanoid metabolism in patients with bacterial sepsis syndrome. |
title_full |
High sPLA2-IIA level is associated with eicosanoid metabolism in patients with bacterial sepsis syndrome. |
title_fullStr |
High sPLA2-IIA level is associated with eicosanoid metabolism in patients with bacterial sepsis syndrome. |
title_full_unstemmed |
High sPLA2-IIA level is associated with eicosanoid metabolism in patients with bacterial sepsis syndrome. |
title_sort |
high spla2-iia level is associated with eicosanoid metabolism in patients with bacterial sepsis syndrome. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2020-01-01 |
description |
The aim of this study was to determine the association between secretory phospholipase A2 group IIA (sPLA2-IIA) and eicosanoid pathway metabolites in patients with bacterial sepsis syndrome (BSS). Levels of sPLA2-IIA, eicosanoids prostaglandin (PG)E2, PGD synthase were quantified in the sera from patients confirmed to have bacterial sepsis (BS; N = 45), bacterial severe sepsis/septic shock (BSS/SS; N = 35) and healthy subjects (N = 45). Cyclooxygenase (COX)-1 and COX-2 activities were analyzed from cell lysate. Serum levels of sPLA2-IIA, PGE2, and PGDS increased significantly in patients with BS and BSS/SS compared to healthy subjects (p<0.05). COX-2 activity was significantly increased in patients with BS compared to healthy subjects (p<0.05), but not COX-1 activity. Binary logistic regression analysis showed that sPLA2-IIA and PGE2 were independent factors predicting BSS severity. In conclusion, high level of sPLA2-IIA is associated with eicosanoid metabolism in patients with BSS. |
url |
https://doi.org/10.1371/journal.pone.0230285 |
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