Evaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acid
Abstract Evaluate the biological action of valproic acid in the acetylation of histones and in the methylation of tumor suppressor genes via oral rinse in patients with a previous history of head and neck squamous cell carcinoma (HNSCC). Forty-two active or former smokers were included in this rando...
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2021-08-01
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Online Access: | https://doi.org/10.1038/s41598-021-95845-3 |
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doaj-a1a750191e93469b91cc45c85575df0e2021-08-15T11:24:52ZengNature Publishing GroupScientific Reports2045-23222021-08-0111111010.1038/s41598-021-95845-3Evaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acidRicardo Ribeiro Gama0Lidia Maria Rebolho Batista Arantes1Bruna Pereira Sorroche2Pedro De Marchi3Matias Eliseo Melendez4Raiany Santos Carvalho5Marcos Alves de Lima6André Luiz Vettore7André Lopes Carvalho8Head and Neck Surgery Department, Barretos Cancer HospitalMolecular Oncology Research Center, Barretos Cancer HospitalMolecular Oncology Research Center, Barretos Cancer HospitalClinical Oncology Department, Barretos Cancer HospitalMolecular Oncology Research Center, Barretos Cancer HospitalResearcher Support Center, Teaching and Research Institute, Barretos Cancer HospitalBiostatistics Center, Teaching and Research Institute, Barretos Cancer HospitalCancer Molecular Biology Laboratory, São Paulo Federal UniversityHead and Neck Surgery Department, Barretos Cancer HospitalAbstract Evaluate the biological action of valproic acid in the acetylation of histones and in the methylation of tumor suppressor genes via oral rinse in patients with a previous history of head and neck squamous cell carcinoma (HNSCC). Forty-two active or former smokers were included in this randomized, double-blind, placebo-controlled trial. Oral rinse samples were collected prior to treatment with valproic acid or placebo and after 90 days of treatment. The methylation status of five tumor suppressor genes and histone acetylation were evaluated by pyrosequencing and ELISA techniques, respectively. Differences between the 90-day and baseline oral rinse acetylation and methylation results were analyzed by comparing groups. Thirty-four patients were considered for analysis. The mean percentage adherence in the valproic and placebo groups was 93.4 and 93.0, respectively (p = 0.718). There was no statistically significant difference between groups when comparing the medians of the histone acetylation ratio and the methylation ratio for most of the studied genes. A significant reduction in the DCC methylation pattern was observed in the valproic group (p = 0.023). The use of valproic acid was safe and accompanied by good therapeutic adherence. DCC methylation was lower in the valproic acid group than in the placebo group.https://doi.org/10.1038/s41598-021-95845-3 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ricardo Ribeiro Gama Lidia Maria Rebolho Batista Arantes Bruna Pereira Sorroche Pedro De Marchi Matias Eliseo Melendez Raiany Santos Carvalho Marcos Alves de Lima André Luiz Vettore André Lopes Carvalho |
spellingShingle |
Ricardo Ribeiro Gama Lidia Maria Rebolho Batista Arantes Bruna Pereira Sorroche Pedro De Marchi Matias Eliseo Melendez Raiany Santos Carvalho Marcos Alves de Lima André Luiz Vettore André Lopes Carvalho Evaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acid Scientific Reports |
author_facet |
Ricardo Ribeiro Gama Lidia Maria Rebolho Batista Arantes Bruna Pereira Sorroche Pedro De Marchi Matias Eliseo Melendez Raiany Santos Carvalho Marcos Alves de Lima André Luiz Vettore André Lopes Carvalho |
author_sort |
Ricardo Ribeiro Gama |
title |
Evaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acid |
title_short |
Evaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acid |
title_full |
Evaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acid |
title_fullStr |
Evaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acid |
title_full_unstemmed |
Evaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acid |
title_sort |
evaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acid |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-08-01 |
description |
Abstract Evaluate the biological action of valproic acid in the acetylation of histones and in the methylation of tumor suppressor genes via oral rinse in patients with a previous history of head and neck squamous cell carcinoma (HNSCC). Forty-two active or former smokers were included in this randomized, double-blind, placebo-controlled trial. Oral rinse samples were collected prior to treatment with valproic acid or placebo and after 90 days of treatment. The methylation status of five tumor suppressor genes and histone acetylation were evaluated by pyrosequencing and ELISA techniques, respectively. Differences between the 90-day and baseline oral rinse acetylation and methylation results were analyzed by comparing groups. Thirty-four patients were considered for analysis. The mean percentage adherence in the valproic and placebo groups was 93.4 and 93.0, respectively (p = 0.718). There was no statistically significant difference between groups when comparing the medians of the histone acetylation ratio and the methylation ratio for most of the studied genes. A significant reduction in the DCC methylation pattern was observed in the valproic group (p = 0.023). The use of valproic acid was safe and accompanied by good therapeutic adherence. DCC methylation was lower in the valproic acid group than in the placebo group. |
url |
https://doi.org/10.1038/s41598-021-95845-3 |
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