Epidemiological investigation and genotypes of Enterocytozoon bieneusi in 11 captive Rhesus macaque populations
Enterocytozoon bieneusi is an obligate intracellular parasite and the most common pathogen of microsporidiosis in humans and animals. In this study, a total of 198 fecal samples were collected from 11 captive populations of Rhesus macaque in Chinese zoos, to investigate the prevalence and analyze th...
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2020-12-01
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doaj-a1ad6cff0c144a1fb52268d167fde52d2020-12-19T05:06:37ZengElsevierInternational Journal for Parasitology: Parasites and Wildlife2213-22442020-12-0113191195Epidemiological investigation and genotypes of Enterocytozoon bieneusi in 11 captive Rhesus macaque populationsMengshi Yu0Xue Liu1Meng Xie2Diyan Li3Qingyong Ni4Mingwang Zhang5Jiayun Wu6Huailiang Xu7Yongfang Yao8College of Life Science, Sichuan Agricultural University, Ya'an, 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya'an, 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya'an, 625014, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, ChinaCollege of Life Science, Sichuan Agricultural University, Ya'an, 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya'an, 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya'an, 625014, China; Corresponding author.Enterocytozoon bieneusi is an obligate intracellular parasite and the most common pathogen of microsporidiosis in humans and animals. In this study, a total of 198 fecal samples were collected from 11 captive populations of Rhesus macaque in Chinese zoos, to investigate the prevalence and analyze the zoonotic potential of E. bieneusi by genotype of the Internal Transcribed Spacer (ITS) gene on the rRNA of E. bieneusi via nested PCR. Results showed that the average infection rate of E. bieneusi in the 11 populations was 13.6%, and the highest infection rate was 56.5% in the population of Xinjiang Tianshan Zoo. Seven genotypes were identified including 2 known genotypes (D and CM1) and 5 novel genotypes (Mul1, Mul2, Mul3, Mul4 and Mul5). Phylogenetic analysis revealed that the novel genotypes Mul2, Mul3, Mul4 and Mul5 belonged to Group 1 showed the zoonotic potential.These findings extend the distribution of E. bieneusi genotypes and provide baseline data for controlling E. bieneusi infection.http://www.sciencedirect.com/science/article/pii/S2213224420300973Enterocytozoon bieneusiMacaca mulattaITSZoonotic potential |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mengshi Yu Xue Liu Meng Xie Diyan Li Qingyong Ni Mingwang Zhang Jiayun Wu Huailiang Xu Yongfang Yao |
spellingShingle |
Mengshi Yu Xue Liu Meng Xie Diyan Li Qingyong Ni Mingwang Zhang Jiayun Wu Huailiang Xu Yongfang Yao Epidemiological investigation and genotypes of Enterocytozoon bieneusi in 11 captive Rhesus macaque populations International Journal for Parasitology: Parasites and Wildlife Enterocytozoon bieneusi Macaca mulatta ITS Zoonotic potential |
author_facet |
Mengshi Yu Xue Liu Meng Xie Diyan Li Qingyong Ni Mingwang Zhang Jiayun Wu Huailiang Xu Yongfang Yao |
author_sort |
Mengshi Yu |
title |
Epidemiological investigation and genotypes of Enterocytozoon bieneusi in 11 captive Rhesus macaque populations |
title_short |
Epidemiological investigation and genotypes of Enterocytozoon bieneusi in 11 captive Rhesus macaque populations |
title_full |
Epidemiological investigation and genotypes of Enterocytozoon bieneusi in 11 captive Rhesus macaque populations |
title_fullStr |
Epidemiological investigation and genotypes of Enterocytozoon bieneusi in 11 captive Rhesus macaque populations |
title_full_unstemmed |
Epidemiological investigation and genotypes of Enterocytozoon bieneusi in 11 captive Rhesus macaque populations |
title_sort |
epidemiological investigation and genotypes of enterocytozoon bieneusi in 11 captive rhesus macaque populations |
publisher |
Elsevier |
series |
International Journal for Parasitology: Parasites and Wildlife |
issn |
2213-2244 |
publishDate |
2020-12-01 |
description |
Enterocytozoon bieneusi is an obligate intracellular parasite and the most common pathogen of microsporidiosis in humans and animals. In this study, a total of 198 fecal samples were collected from 11 captive populations of Rhesus macaque in Chinese zoos, to investigate the prevalence and analyze the zoonotic potential of E. bieneusi by genotype of the Internal Transcribed Spacer (ITS) gene on the rRNA of E. bieneusi via nested PCR. Results showed that the average infection rate of E. bieneusi in the 11 populations was 13.6%, and the highest infection rate was 56.5% in the population of Xinjiang Tianshan Zoo. Seven genotypes were identified including 2 known genotypes (D and CM1) and 5 novel genotypes (Mul1, Mul2, Mul3, Mul4 and Mul5). Phylogenetic analysis revealed that the novel genotypes Mul2, Mul3, Mul4 and Mul5 belonged to Group 1 showed the zoonotic potential.These findings extend the distribution of E. bieneusi genotypes and provide baseline data for controlling E. bieneusi infection. |
topic |
Enterocytozoon bieneusi Macaca mulatta ITS Zoonotic potential |
url |
http://www.sciencedirect.com/science/article/pii/S2213224420300973 |
work_keys_str_mv |
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