miR-93, miR-373, and miR-17-5p Negatively Regulate the Expression of TBP2 in Lung Cancer
Recently, several miRNAs have been revealed to play critical roles in oncogenesis and tumor progression of many cancers. Thioredoxin-1 (Trx-1) binding protein-2 (TBP-2) is an internal inhibitor of Trx-1, which plays the role in regulating oxidative stress, inhibiting cell growth, and promoting apopt...
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doaj-a1b59d7a07a9400da2d8bb840ff4a7822020-11-25T03:49:29ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2020-04-011010.3389/fonc.2020.00526511291miR-93, miR-373, and miR-17-5p Negatively Regulate the Expression of TBP2 in Lung CancerYe Li0Min Liang1Yunhui Zhang2Bing Yuan3Wenchao Gao4Zhizhou Shi5Jie Bai6Medical Faculty, Kunming University of Science and Technology, Kunming, ChinaMedical Faculty, Kunming University of Science and Technology, Kunming, ChinaMedical Faculty, Kunming University of Science and Technology, Kunming, ChinaFirst People's Hospital of Yunnan Province, Kunming, ChinaMedical Faculty, Kunming University of Science and Technology, Kunming, ChinaMedical Faculty, Kunming University of Science and Technology, Kunming, ChinaMedical Faculty, Kunming University of Science and Technology, Kunming, ChinaRecently, several miRNAs have been revealed to play critical roles in oncogenesis and tumor progression of many cancers. Thioredoxin-1 (Trx-1) binding protein-2 (TBP-2) is an internal inhibitor of Trx-1, which plays the role in regulating oxidative stress, inhibiting cell growth, and promoting apoptosis. The expression of TBP-2 is usually decreased in cancer tissues. However, whether the miRNAs regulate the TBP-2 expression in lung cancer is still unclear. In this study, we examined the levels of TBP-2, miR-93, miR-373, and miR-17-5p in lung cancer tissues and their adjacent normal lung tissues of 36 patients. We found that the expressions of miR-93, miR-373, and miR-17-5p were higher, whereas the expression of TBP-2 mRNA and protein was significantly lower in lung cancer tissues compared with adjacent normal lung tissues. After the three miRNA mimics were transfected in the lung cancer cells, NCI-H460, the level of TBP-2 mRNA and TBP-2 protein was decreased. Then, the anti-cancer drug 5-fluorouracil was used to stimulate the NCI-H460 cells; the mRNA levels of miR-93, miR-373, and miR-17-5p were decreased, and the level of TBP-2 mRNA and protein was increased. Collectively, the above results suggest that miR-93, miR-373, and miR-17-5p negatively regulate the TBP-2 expression in lung cancer. This study may provide therapeutic targets with lung cancer.https://www.frontiersin.org/article/10.3389/fonc.2020.00526/fulllung cancerTBP-2miR-93miR-373miR-17-5p |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ye Li Min Liang Yunhui Zhang Bing Yuan Wenchao Gao Zhizhou Shi Jie Bai |
spellingShingle |
Ye Li Min Liang Yunhui Zhang Bing Yuan Wenchao Gao Zhizhou Shi Jie Bai miR-93, miR-373, and miR-17-5p Negatively Regulate the Expression of TBP2 in Lung Cancer Frontiers in Oncology lung cancer TBP-2 miR-93 miR-373 miR-17-5p |
author_facet |
Ye Li Min Liang Yunhui Zhang Bing Yuan Wenchao Gao Zhizhou Shi Jie Bai |
author_sort |
Ye Li |
title |
miR-93, miR-373, and miR-17-5p Negatively Regulate the Expression of TBP2 in Lung Cancer |
title_short |
miR-93, miR-373, and miR-17-5p Negatively Regulate the Expression of TBP2 in Lung Cancer |
title_full |
miR-93, miR-373, and miR-17-5p Negatively Regulate the Expression of TBP2 in Lung Cancer |
title_fullStr |
miR-93, miR-373, and miR-17-5p Negatively Regulate the Expression of TBP2 in Lung Cancer |
title_full_unstemmed |
miR-93, miR-373, and miR-17-5p Negatively Regulate the Expression of TBP2 in Lung Cancer |
title_sort |
mir-93, mir-373, and mir-17-5p negatively regulate the expression of tbp2 in lung cancer |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Oncology |
issn |
2234-943X |
publishDate |
2020-04-01 |
description |
Recently, several miRNAs have been revealed to play critical roles in oncogenesis and tumor progression of many cancers. Thioredoxin-1 (Trx-1) binding protein-2 (TBP-2) is an internal inhibitor of Trx-1, which plays the role in regulating oxidative stress, inhibiting cell growth, and promoting apoptosis. The expression of TBP-2 is usually decreased in cancer tissues. However, whether the miRNAs regulate the TBP-2 expression in lung cancer is still unclear. In this study, we examined the levels of TBP-2, miR-93, miR-373, and miR-17-5p in lung cancer tissues and their adjacent normal lung tissues of 36 patients. We found that the expressions of miR-93, miR-373, and miR-17-5p were higher, whereas the expression of TBP-2 mRNA and protein was significantly lower in lung cancer tissues compared with adjacent normal lung tissues. After the three miRNA mimics were transfected in the lung cancer cells, NCI-H460, the level of TBP-2 mRNA and TBP-2 protein was decreased. Then, the anti-cancer drug 5-fluorouracil was used to stimulate the NCI-H460 cells; the mRNA levels of miR-93, miR-373, and miR-17-5p were decreased, and the level of TBP-2 mRNA and protein was increased. Collectively, the above results suggest that miR-93, miR-373, and miR-17-5p negatively regulate the TBP-2 expression in lung cancer. This study may provide therapeutic targets with lung cancer. |
topic |
lung cancer TBP-2 miR-93 miR-373 miR-17-5p |
url |
https://www.frontiersin.org/article/10.3389/fonc.2020.00526/full |
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