No evidence for change in expression of TBC1D1 and TBC1D4 genes in cultured human adipocytes stimulated by myokines and adipokines
TBC1D1 and TBC1D4 proteins play analogous, but not identical role in governing insulin-signalling pathway. Little is known about changes in expression levels of TBC1D1 and TBC1D4 genes in mammals, including humans. Number of factors were studied, but data remain controversial. The aim of this study...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2021-01-01
|
Series: | Adipocyte |
Subjects: | |
Online Access: | http://dx.doi.org/10.1080/21623945.2021.1900497 |
id |
doaj-a1c5a3b24d2942b3bdeb251b5d099437 |
---|---|
record_format |
Article |
spelling |
doaj-a1c5a3b24d2942b3bdeb251b5d0994372021-04-06T13:27:32ZengTaylor & Francis GroupAdipocyte2162-39452162-397X2021-01-0110115315910.1080/21623945.2021.19004971900497No evidence for change in expression of TBC1D1 and TBC1D4 genes in cultured human adipocytes stimulated by myokines and adipokinesŁukasz Kępczyński0Szymon Wcisło1Irmina Korzeniewska-Dyl2Katarzyna Połatyńska3Agnieszka Gach4Dariusz Moczulski5Polish Mothers’ Memorial Institute Research HospitalMedical University of Łódź and Military Medical Academy Memorial Teaching Hospital of the Medical University of Łódź - Central Veteran HospitalMedical University of Łódź and Military Medical Academy Memorial Teaching Hospital of the Medical University of Łódź - Central Veteran HospitalPolish Mothers’ Memorial Institute Research HospitalPolish Mothers’ Memorial Institute Research HospitalMedical University of Łódź and Military Medical Academy Memorial Teaching Hospital of the Medical University of Łódź - Central Veteran HospitalTBC1D1 and TBC1D4 proteins play analogous, but not identical role in governing insulin-signalling pathway. Little is known about changes in expression levels of TBC1D1 and TBC1D4 genes in mammals, including humans. Number of factors were studied, but data remain controversial. The aim of this study was to evaluate the effect of selected cytokines, adipokines and myokines with known or putative insulin sensitivity regulation activity (adiponectin, irisin, omentin, interleukin 6, leptin, resistin, and tumour necrosis factor) on TBC1D1 and TBC1D4 expression levels in cultured differentiated human adipocytes. No significant differences were found between the adipocytes treated with different stimuli and this effect was determined not dose dependent. It is reasonable to conclude that relative shortage of data showing no change in TBC1D1 and TBC1D4 from literature results from publication bias; therefore, our finding provides additional insight into the role of both genes.http://dx.doi.org/10.1080/21623945.2021.1900497tbc1d1tbc1d4cytokinesadipokinesirisin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Łukasz Kępczyński Szymon Wcisło Irmina Korzeniewska-Dyl Katarzyna Połatyńska Agnieszka Gach Dariusz Moczulski |
spellingShingle |
Łukasz Kępczyński Szymon Wcisło Irmina Korzeniewska-Dyl Katarzyna Połatyńska Agnieszka Gach Dariusz Moczulski No evidence for change in expression of TBC1D1 and TBC1D4 genes in cultured human adipocytes stimulated by myokines and adipokines Adipocyte tbc1d1 tbc1d4 cytokines adipokines irisin |
author_facet |
Łukasz Kępczyński Szymon Wcisło Irmina Korzeniewska-Dyl Katarzyna Połatyńska Agnieszka Gach Dariusz Moczulski |
author_sort |
Łukasz Kępczyński |
title |
No evidence for change in expression of TBC1D1 and TBC1D4 genes in cultured human adipocytes stimulated by myokines and adipokines |
title_short |
No evidence for change in expression of TBC1D1 and TBC1D4 genes in cultured human adipocytes stimulated by myokines and adipokines |
title_full |
No evidence for change in expression of TBC1D1 and TBC1D4 genes in cultured human adipocytes stimulated by myokines and adipokines |
title_fullStr |
No evidence for change in expression of TBC1D1 and TBC1D4 genes in cultured human adipocytes stimulated by myokines and adipokines |
title_full_unstemmed |
No evidence for change in expression of TBC1D1 and TBC1D4 genes in cultured human adipocytes stimulated by myokines and adipokines |
title_sort |
no evidence for change in expression of tbc1d1 and tbc1d4 genes in cultured human adipocytes stimulated by myokines and adipokines |
publisher |
Taylor & Francis Group |
series |
Adipocyte |
issn |
2162-3945 2162-397X |
publishDate |
2021-01-01 |
description |
TBC1D1 and TBC1D4 proteins play analogous, but not identical role in governing insulin-signalling pathway. Little is known about changes in expression levels of TBC1D1 and TBC1D4 genes in mammals, including humans. Number of factors were studied, but data remain controversial. The aim of this study was to evaluate the effect of selected cytokines, adipokines and myokines with known or putative insulin sensitivity regulation activity (adiponectin, irisin, omentin, interleukin 6, leptin, resistin, and tumour necrosis factor) on TBC1D1 and TBC1D4 expression levels in cultured differentiated human adipocytes. No significant differences were found between the adipocytes treated with different stimuli and this effect was determined not dose dependent. It is reasonable to conclude that relative shortage of data showing no change in TBC1D1 and TBC1D4 from literature results from publication bias; therefore, our finding provides additional insight into the role of both genes. |
topic |
tbc1d1 tbc1d4 cytokines adipokines irisin |
url |
http://dx.doi.org/10.1080/21623945.2021.1900497 |
work_keys_str_mv |
AT łukaszkepczynski noevidenceforchangeinexpressionoftbc1d1andtbc1d4genesinculturedhumanadipocytesstimulatedbymyokinesandadipokines AT szymonwcisło noevidenceforchangeinexpressionoftbc1d1andtbc1d4genesinculturedhumanadipocytesstimulatedbymyokinesandadipokines AT irminakorzeniewskadyl noevidenceforchangeinexpressionoftbc1d1andtbc1d4genesinculturedhumanadipocytesstimulatedbymyokinesandadipokines AT katarzynapołatynska noevidenceforchangeinexpressionoftbc1d1andtbc1d4genesinculturedhumanadipocytesstimulatedbymyokinesandadipokines AT agnieszkagach noevidenceforchangeinexpressionoftbc1d1andtbc1d4genesinculturedhumanadipocytesstimulatedbymyokinesandadipokines AT dariuszmoczulski noevidenceforchangeinexpressionoftbc1d1andtbc1d4genesinculturedhumanadipocytesstimulatedbymyokinesandadipokines |
_version_ |
1721538244141645824 |