Matrix-assisted laser desorption ionization-time of flight: a promising alternative method of identifying the major coagulase-negative Staphylococci species

Matrix-assisted laser desorption ionization-time of flight: a promising alternative method of identifying the major coagulase-negative Staphylococci species

Abstract INTRODUCTION. This study aimed to evaluate different methods for differentiation of species of coagulase-negative staphylococci (CoNS) that caused infections in hospitalized immunocompromised patients. METHODS. A total of 134 CoNS strains were characterized using four different methods....

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Bibliographic Details
Main Authors: Lucas Eduardo Trevisoli, Larissa Bail, Luiza Souza Rodrigues, Danieli Conte, Jussara Kasuko Palmeiro, Libera Maria Dalla-Costa
Format: Article
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT)
Series:Revista da Sociedade Brasileira de Medicina Tropical
Subjects:
Coagulase-negative staphylococci
MALDI-TOF
tuf gene
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000100085&lng=en&tlng=en
Description
Summary:Abstract INTRODUCTION. This study aimed to evaluate different methods for differentiation of species of coagulase-negative staphylococci (CoNS) that caused infections in hospitalized immunocompromised patients. METHODS. A total of 134 CoNS strains were characterized using four different methods. RESULTS. The results of matrix assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) analysis were in complete agreement with those of tuf gene sequencing (kappa index = 1.00). The kappa index of Vitek 2® Compact analysis was 0.85 (very good) and that of the conventional method was 0.63 (moderate). CONCLUSIONS . MALDI-TOF MS provided rapid and accurate results for the identification of CoNS (134; 100%).
ISSN:1678-9849

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