Evaluation of possible biomarkers for caries risk in children 6 to 12 years of age
Background: Electrolytes, proteins, and other salivary molecules play an important role in tooth integrity and can serve as biomarkers associated with caries. Objective: To determine the concentration of potential biomarkers in children without caries (CF) and children with caries (CA). Methods: Uns...
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doaj-a1fd4b1ff0854364a833d88a37a9d9be2021-08-24T14:41:00ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972021-01-0113110.1080/20002297.2021.19562191956219Evaluation of possible biomarkers for caries risk in children 6 to 12 years of ageMaría P Angarita-Díaz0Aurea Simon-Soro1Diana Forero2Felipe Balcázar3Luisa Sarmiento4Erika Romero5Alex Mira6Universidad Cooperativa De ColombiaFoundation for the Promotion of Health and Biomedical ResearchUniversidad Cooperativa De ColombiaUniversidad Cooperativa De ColombiaUniversidad Cooperativa De ColombiaUniversidad Cooperativa De ColombiaFoundation for the Promotion of Health and Biomedical ResearchBackground: Electrolytes, proteins, and other salivary molecules play an important role in tooth integrity and can serve as biomarkers associated with caries. Objective: To determine the concentration of potential biomarkers in children without caries (CF) and children with caries (CA). Methods: Unstimulated saliva was collected, and the biomarkers quantified in duplicate, using commercial Enzyme Linked Immunosorbent Assay (ELISA) kits to determine IgA, fibronectin, cathelicidin LL-37, and statherin levels, as well as colorimetric tests to detect formate and phosphate. Results: Significantly higher concentrations of statherin was detected in the CF group (Median: 94,734.6; IQR: 92,934.6–95,113.7) compared to the CA2 group (90,875.0; IQR: 83,580.2–94,633.4) (p = 0.03). Slightly higher median IgA (48,250.0; IQR: 31,461.9–67,418.8) and LL-37 levels (56.1; IQR 43.6–116.2) and a lower concentration of formate were detected in the CF group (0.02; IQR 0.0034–0.15) compared to the group with caries (IgA: 37,776.42; IQR: 33,383.9–44,128.5; LL-37: 46.3; IQR: 40.1011–67.7; formate: 0.10; IQR: 0.01–0.18), but these differences were not statistically significant. Conclusion: The fact that these compounds have been identified as good markers for caries among European adults highlights the difficulty of identifying universal biomarkers that are applicable to all ages or to different populations.http://dx.doi.org/10.1080/20002297.2021.1956219biomarkerschilddental cariessaliva |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
María P Angarita-Díaz Aurea Simon-Soro Diana Forero Felipe Balcázar Luisa Sarmiento Erika Romero Alex Mira |
spellingShingle |
María P Angarita-Díaz Aurea Simon-Soro Diana Forero Felipe Balcázar Luisa Sarmiento Erika Romero Alex Mira Evaluation of possible biomarkers for caries risk in children 6 to 12 years of age Journal of Oral Microbiology biomarkers child dental caries saliva |
author_facet |
María P Angarita-Díaz Aurea Simon-Soro Diana Forero Felipe Balcázar Luisa Sarmiento Erika Romero Alex Mira |
author_sort |
María P Angarita-Díaz |
title |
Evaluation of possible biomarkers for caries risk in children 6 to 12 years of age |
title_short |
Evaluation of possible biomarkers for caries risk in children 6 to 12 years of age |
title_full |
Evaluation of possible biomarkers for caries risk in children 6 to 12 years of age |
title_fullStr |
Evaluation of possible biomarkers for caries risk in children 6 to 12 years of age |
title_full_unstemmed |
Evaluation of possible biomarkers for caries risk in children 6 to 12 years of age |
title_sort |
evaluation of possible biomarkers for caries risk in children 6 to 12 years of age |
publisher |
Taylor & Francis Group |
series |
Journal of Oral Microbiology |
issn |
2000-2297 |
publishDate |
2021-01-01 |
description |
Background: Electrolytes, proteins, and other salivary molecules play an important role in tooth integrity and can serve as biomarkers associated with caries. Objective: To determine the concentration of potential biomarkers in children without caries (CF) and children with caries (CA). Methods: Unstimulated saliva was collected, and the biomarkers quantified in duplicate, using commercial Enzyme Linked Immunosorbent Assay (ELISA) kits to determine IgA, fibronectin, cathelicidin LL-37, and statherin levels, as well as colorimetric tests to detect formate and phosphate. Results: Significantly higher concentrations of statherin was detected in the CF group (Median: 94,734.6; IQR: 92,934.6–95,113.7) compared to the CA2 group (90,875.0; IQR: 83,580.2–94,633.4) (p = 0.03). Slightly higher median IgA (48,250.0; IQR: 31,461.9–67,418.8) and LL-37 levels (56.1; IQR 43.6–116.2) and a lower concentration of formate were detected in the CF group (0.02; IQR 0.0034–0.15) compared to the group with caries (IgA: 37,776.42; IQR: 33,383.9–44,128.5; LL-37: 46.3; IQR: 40.1011–67.7; formate: 0.10; IQR: 0.01–0.18), but these differences were not statistically significant. Conclusion: The fact that these compounds have been identified as good markers for caries among European adults highlights the difficulty of identifying universal biomarkers that are applicable to all ages or to different populations. |
topic |
biomarkers child dental caries saliva |
url |
http://dx.doi.org/10.1080/20002297.2021.1956219 |
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