Establishment and Evaluation of a Rat Model of Sidestream Cigarette Smoke-Induced Chronic Obstructive Pulmonary Disease
Chronic obstructive pulmonary disease (COPD) is a common cause of mortality worldwide. The current lack of an animal model that can be established within a certain time frame and imitate the unique features of the disease is a major limiting factor in its study. The present study established and eva...
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Frontiers Media S.A.
2018-02-01
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Series: | Frontiers in Physiology |
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Online Access: | http://journal.frontiersin.org/article/10.3389/fphys.2018.00058/full |
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Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Genfa Wang Genfa Wang Genfa Wang Nabijan Mohammadtursun Nabijan Mohammadtursun Nabijan Mohammadtursun Jing Sun Jing Sun Yubao Lv Yubao Lv Hualiang Jin Hualiang Jin Jinpei Lin Jinpei Lin Lingwen Kong Lingwen Kong Zhengxiao Zhao Zhengxiao Zhao Hongying Zhang Hongying Zhang Jingcheng Dong Jingcheng Dong |
spellingShingle |
Genfa Wang Genfa Wang Genfa Wang Nabijan Mohammadtursun Nabijan Mohammadtursun Nabijan Mohammadtursun Jing Sun Jing Sun Yubao Lv Yubao Lv Hualiang Jin Hualiang Jin Jinpei Lin Jinpei Lin Lingwen Kong Lingwen Kong Zhengxiao Zhao Zhengxiao Zhao Hongying Zhang Hongying Zhang Jingcheng Dong Jingcheng Dong Establishment and Evaluation of a Rat Model of Sidestream Cigarette Smoke-Induced Chronic Obstructive Pulmonary Disease Frontiers in Physiology chronic obstructive pulmonary disease passive smoking cigarette smoke rat model airway remodeling |
author_facet |
Genfa Wang Genfa Wang Genfa Wang Nabijan Mohammadtursun Nabijan Mohammadtursun Nabijan Mohammadtursun Jing Sun Jing Sun Yubao Lv Yubao Lv Hualiang Jin Hualiang Jin Jinpei Lin Jinpei Lin Lingwen Kong Lingwen Kong Zhengxiao Zhao Zhengxiao Zhao Hongying Zhang Hongying Zhang Jingcheng Dong Jingcheng Dong |
author_sort |
Genfa Wang |
title |
Establishment and Evaluation of a Rat Model of Sidestream Cigarette Smoke-Induced Chronic Obstructive Pulmonary Disease |
title_short |
Establishment and Evaluation of a Rat Model of Sidestream Cigarette Smoke-Induced Chronic Obstructive Pulmonary Disease |
title_full |
Establishment and Evaluation of a Rat Model of Sidestream Cigarette Smoke-Induced Chronic Obstructive Pulmonary Disease |
title_fullStr |
Establishment and Evaluation of a Rat Model of Sidestream Cigarette Smoke-Induced Chronic Obstructive Pulmonary Disease |
title_full_unstemmed |
Establishment and Evaluation of a Rat Model of Sidestream Cigarette Smoke-Induced Chronic Obstructive Pulmonary Disease |
title_sort |
establishment and evaluation of a rat model of sidestream cigarette smoke-induced chronic obstructive pulmonary disease |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Physiology |
issn |
1664-042X |
publishDate |
2018-02-01 |
description |
Chronic obstructive pulmonary disease (COPD) is a common cause of mortality worldwide. The current lack of an animal model that can be established within a certain time frame and imitate the unique features of the disease is a major limiting factor in its study. The present study established and evaluated an animal model of COPD that represents the early and advanced stage features using short-, middle-, and long-term sidestream cigarette smoke (CS) exposure. One hundred and nine Sprague–Dawley rats were randomly divided into 10 groups for different periods of sidestream CS exposure or no exposure (i.e., normal groups). The rats were exposed to CS from 3R4F cigarettes in an exposure chamber. Histological analysis was performed to determine pathological changes. We also conducted open-field tests, lung function evaluations, and cytokine analysis of the blood serum, bronchoalveolar lavage fluid, and lung tissue. The lung tissue protein levels, blood gases, and were also analyzed. As the CS exposure time increased, the indicators associated with oxidative stress, inflammatory responses, and airway remodeling were greater in the CS exposure groups than in the normal group. At 24 and 36 weeks, the COPD model rats displayed the middle- and advanced-stage features of COPD, respectively. In the 8-week CS exposure group, after the CS exposure was stopped for 4 weeks, inflammatory responses and oxidative responses were ameliorated and lung function exacerbation was reduced compared with the 12-week CS exposure group. Therefore, we established a more adequate rat model of sidestream CS induced COPD, which will have great significance for a better understanding of the pathogenesis of COPD and drug effectiveness evaluation. |
topic |
chronic obstructive pulmonary disease passive smoking cigarette smoke rat model airway remodeling |
url |
http://journal.frontiersin.org/article/10.3389/fphys.2018.00058/full |
work_keys_str_mv |
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doaj-a1ff83d7b6474969b6b1f68198142c252020-11-24T21:24:40ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-02-01910.3389/fphys.2018.00058326122Establishment and Evaluation of a Rat Model of Sidestream Cigarette Smoke-Induced Chronic Obstructive Pulmonary DiseaseGenfa Wang0Genfa Wang1Genfa Wang2Nabijan Mohammadtursun3Nabijan Mohammadtursun4Nabijan Mohammadtursun5Jing Sun6Jing Sun7Yubao Lv8Yubao Lv9Hualiang Jin10Hualiang Jin11Jinpei Lin12Jinpei Lin13Lingwen Kong14Lingwen Kong15Zhengxiao Zhao16Zhengxiao Zhao17Hongying Zhang18Hongying Zhang19Jingcheng Dong20Jingcheng Dong21Department of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaThe Institutes of Integrative Medicine, Fudan University, Shanghai, ChinaDepartment of TCM, The Second Affiliated Hospital of Nanchang University, Nanchang, ChinaDepartment of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaThe Institutes of Integrative Medicine, Fudan University, Shanghai, ChinaCollege of Xinjiang Uyghur Medicine, Hotan, ChinaDepartment of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaThe Institutes of Integrative Medicine, Fudan University, Shanghai, ChinaDepartment of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaThe Institutes of Integrative Medicine, Fudan University, Shanghai, ChinaDepartment of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaThe Institutes of Integrative Medicine, Fudan University, Shanghai, ChinaDepartment of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaThe Institutes of Integrative Medicine, Fudan University, Shanghai, ChinaDepartment of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaThe Institutes of Integrative Medicine, Fudan University, Shanghai, ChinaDepartment of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaThe Institutes of Integrative Medicine, Fudan University, Shanghai, ChinaDepartment of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaThe Institutes of Integrative Medicine, Fudan University, Shanghai, ChinaDepartment of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaThe Institutes of Integrative Medicine, Fudan University, Shanghai, ChinaChronic obstructive pulmonary disease (COPD) is a common cause of mortality worldwide. The current lack of an animal model that can be established within a certain time frame and imitate the unique features of the disease is a major limiting factor in its study. The present study established and evaluated an animal model of COPD that represents the early and advanced stage features using short-, middle-, and long-term sidestream cigarette smoke (CS) exposure. One hundred and nine Sprague–Dawley rats were randomly divided into 10 groups for different periods of sidestream CS exposure or no exposure (i.e., normal groups). The rats were exposed to CS from 3R4F cigarettes in an exposure chamber. Histological analysis was performed to determine pathological changes. We also conducted open-field tests, lung function evaluations, and cytokine analysis of the blood serum, bronchoalveolar lavage fluid, and lung tissue. The lung tissue protein levels, blood gases, and were also analyzed. As the CS exposure time increased, the indicators associated with oxidative stress, inflammatory responses, and airway remodeling were greater in the CS exposure groups than in the normal group. At 24 and 36 weeks, the COPD model rats displayed the middle- and advanced-stage features of COPD, respectively. In the 8-week CS exposure group, after the CS exposure was stopped for 4 weeks, inflammatory responses and oxidative responses were ameliorated and lung function exacerbation was reduced compared with the 12-week CS exposure group. Therefore, we established a more adequate rat model of sidestream CS induced COPD, which will have great significance for a better understanding of the pathogenesis of COPD and drug effectiveness evaluation.http://journal.frontiersin.org/article/10.3389/fphys.2018.00058/fullchronic obstructive pulmonary diseasepassive smokingcigarette smokerat modelairway remodeling |