The Role of Butyrate on Monocyte Migration and Inflammation Response in Patient with Type 2 Diabetes Mellitus
Type 2 Diabetes Mellitus (T2DM) is a very serious global problem. In Indonesia, this disease attacks at the most productive age; consequently, it can reduce economic status and life expectancy. The pathogenesis of T2DM is very closely related to inflammation and macrophage accumulation. However, no...
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doaj-a20a10064cff4d2a912dad6d5c5c68682020-11-25T02:14:53ZengMDPI AGBiomedicines2227-90592019-09-01747410.3390/biomedicines7040074biomedicines7040074The Role of Butyrate on Monocyte Migration and Inflammation Response in Patient with Type 2 Diabetes MellitusRahma Ayu Larasati0Dante Saksono Harbuwono1Ekowati Rahajeng2Saraswati Pradipta3Hanny Siti Nuraeni4Andi Susilowati5Heri Wibowo6Magister Program of Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta 10440, IndonesiaInternal Medicine Department, Faculty of Medicine, Universitas Indonesia, Jakarta 10440, IndonesiaMinistry of Health Republic of Indonesia, Jakarta 10560, IndonesiaMagister Program of Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta 10440, IndonesiaMagister Program of Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta 10440, IndonesiaMinistry of Health Republic of Indonesia, Jakarta 10560, IndonesiaMagister Program of Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta 10440, IndonesiaType 2 Diabetes Mellitus (T2DM) is a very serious global problem. In Indonesia, this disease attacks at the most productive age; consequently, it can reduce economic status and life expectancy. The pathogenesis of T2DM is very closely related to inflammation and macrophage accumulation. However, no anti-inflammatory agent has been proven to play a role in the management of T2DM. Butyrate is a short chain fatty acid produced from resistant starch fermentation in the intestinal lumen. It is able to bind to GPR41 and GPR43 receptors on monocytes, so that it can change the pattern of cytokine expression, activation, migration and cell differentiation. Hence, it is interesting to examine the anti-inflammation effect of butyrate and the effect on monocyte migration. A total of 37 subjects were examined in this study. They were divided into two groups, with and without butyrate treatment. We analyzed two pro-inflammatory cytokines (Tumor Necrosis Factor TNF-α and Interleukin IL-6) and one anti-inflammatory cytokine, IL 10. Monocytes were isolated in type 1 collagen gel for migration testing using the µ-slide chemotaxis IBIDI. Image analysis used ImageJ and Chemotaxis tool software. There was a significant difference in the TNFα/IL 10 ratio between healthy groups and T2DM. Butyrate also appears to suppress TNFα cytokine production and increase IL10 production. It also decreases the accumulation distance of monocyte migration in T2DM.https://www.mdpi.com/2227-9059/7/4/74diabetes mellitusbutyrateinflammationmonocyte migration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rahma Ayu Larasati Dante Saksono Harbuwono Ekowati Rahajeng Saraswati Pradipta Hanny Siti Nuraeni Andi Susilowati Heri Wibowo |
spellingShingle |
Rahma Ayu Larasati Dante Saksono Harbuwono Ekowati Rahajeng Saraswati Pradipta Hanny Siti Nuraeni Andi Susilowati Heri Wibowo The Role of Butyrate on Monocyte Migration and Inflammation Response in Patient with Type 2 Diabetes Mellitus Biomedicines diabetes mellitus butyrate inflammation monocyte migration |
author_facet |
Rahma Ayu Larasati Dante Saksono Harbuwono Ekowati Rahajeng Saraswati Pradipta Hanny Siti Nuraeni Andi Susilowati Heri Wibowo |
author_sort |
Rahma Ayu Larasati |
title |
The Role of Butyrate on Monocyte Migration and Inflammation Response in Patient with Type 2 Diabetes Mellitus |
title_short |
The Role of Butyrate on Monocyte Migration and Inflammation Response in Patient with Type 2 Diabetes Mellitus |
title_full |
The Role of Butyrate on Monocyte Migration and Inflammation Response in Patient with Type 2 Diabetes Mellitus |
title_fullStr |
The Role of Butyrate on Monocyte Migration and Inflammation Response in Patient with Type 2 Diabetes Mellitus |
title_full_unstemmed |
The Role of Butyrate on Monocyte Migration and Inflammation Response in Patient with Type 2 Diabetes Mellitus |
title_sort |
role of butyrate on monocyte migration and inflammation response in patient with type 2 diabetes mellitus |
publisher |
MDPI AG |
series |
Biomedicines |
issn |
2227-9059 |
publishDate |
2019-09-01 |
description |
Type 2 Diabetes Mellitus (T2DM) is a very serious global problem. In Indonesia, this disease attacks at the most productive age; consequently, it can reduce economic status and life expectancy. The pathogenesis of T2DM is very closely related to inflammation and macrophage accumulation. However, no anti-inflammatory agent has been proven to play a role in the management of T2DM. Butyrate is a short chain fatty acid produced from resistant starch fermentation in the intestinal lumen. It is able to bind to GPR41 and GPR43 receptors on monocytes, so that it can change the pattern of cytokine expression, activation, migration and cell differentiation. Hence, it is interesting to examine the anti-inflammation effect of butyrate and the effect on monocyte migration. A total of 37 subjects were examined in this study. They were divided into two groups, with and without butyrate treatment. We analyzed two pro-inflammatory cytokines (Tumor Necrosis Factor TNF-α and Interleukin IL-6) and one anti-inflammatory cytokine, IL 10. Monocytes were isolated in type 1 collagen gel for migration testing using the µ-slide chemotaxis IBIDI. Image analysis used ImageJ and Chemotaxis tool software. There was a significant difference in the TNFα/IL 10 ratio between healthy groups and T2DM. Butyrate also appears to suppress TNFα cytokine production and increase IL10 production. It also decreases the accumulation distance of monocyte migration in T2DM. |
topic |
diabetes mellitus butyrate inflammation monocyte migration |
url |
https://www.mdpi.com/2227-9059/7/4/74 |
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