Evaluation of tissue reaction to Aroeira (Myracrodruon urundeuva) extracts: a histologic and edemogenic study
OBJECTIVES: This study evaluated subcutaneous tissue response to Aroeira (Myracrodruon urundeuva) extract employing edemogenic and histological analyses. MATERIAL AND METHODS: Test groups consisted of aqueous and ethanolic Aroeira extracts and saline (control). For edema quantification, 18 rats rece...
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University of São Paulo
2012-08-01
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doaj-a20bab71cc3745f0bea1e7d7a66b37b52020-11-24T22:59:15ZengUniversity of São PauloJournal of Applied Oral Science1678-77571678-77652012-08-0120441441810.1590/S1678-77572012000400005Evaluation of tissue reaction to Aroeira (Myracrodruon urundeuva) extracts: a histologic and edemogenic studyAlessandra Cury MachadoEloi Dezan JuniorJoão Eduardo Gomes-FilhoLuciano Tavares Angelo CintraDenise Belucio RuviéreRenata ZoccalCarla Andreotti DamanteElerson Gaetti Jardim JuniorOBJECTIVES: This study evaluated subcutaneous tissue response to Aroeira (Myracrodruon urundeuva) extract employing edemogenic and histological analyses. MATERIAL AND METHODS: Test groups consisted of aqueous and ethanolic Aroeira extracts and saline (control). For edema quantification, 18 rats received an intravenous injection of Evan's Blue. After 30 min, the extracts and saline were injected on the dorsum of the rats, which were then sacrificed after 3 and 6 h. Readings were performed in a spectrophotometer. For subcutaneous implantation, 30 rats received a polyethylene tube containing the extracts on their dorsum and then they were killed after 7 and 28 days. The samples were processed for histological analysis and evaluated with a light microscope. The inflammatory infiltrate was quantified. RESULTS: There were no statistically significant differences between aqueous extract and saline groups in relation to edema quantification in the different periods (p>0.05). Ethanolic solution resulted in more edema independently of the experimental period (p<0.05). Histological analysis showed similar results on the 7-day period for the 3 groups. There was a notable reduction on inflammatory cell number for saline and aqueous extract groups at 28 days. CONCLUSION: The aqueous extract showed biocompatible properties similar to those of saline.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572012000400005InflammationEdemaMedicinal plantsAnacardiaceae |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alessandra Cury Machado Eloi Dezan Junior João Eduardo Gomes-Filho Luciano Tavares Angelo Cintra Denise Belucio Ruviére Renata Zoccal Carla Andreotti Damante Elerson Gaetti Jardim Junior |
spellingShingle |
Alessandra Cury Machado Eloi Dezan Junior João Eduardo Gomes-Filho Luciano Tavares Angelo Cintra Denise Belucio Ruviére Renata Zoccal Carla Andreotti Damante Elerson Gaetti Jardim Junior Evaluation of tissue reaction to Aroeira (Myracrodruon urundeuva) extracts: a histologic and edemogenic study Journal of Applied Oral Science Inflammation Edema Medicinal plants Anacardiaceae |
author_facet |
Alessandra Cury Machado Eloi Dezan Junior João Eduardo Gomes-Filho Luciano Tavares Angelo Cintra Denise Belucio Ruviére Renata Zoccal Carla Andreotti Damante Elerson Gaetti Jardim Junior |
author_sort |
Alessandra Cury Machado |
title |
Evaluation of tissue reaction to Aroeira (Myracrodruon urundeuva) extracts: a histologic and edemogenic study |
title_short |
Evaluation of tissue reaction to Aroeira (Myracrodruon urundeuva) extracts: a histologic and edemogenic study |
title_full |
Evaluation of tissue reaction to Aroeira (Myracrodruon urundeuva) extracts: a histologic and edemogenic study |
title_fullStr |
Evaluation of tissue reaction to Aroeira (Myracrodruon urundeuva) extracts: a histologic and edemogenic study |
title_full_unstemmed |
Evaluation of tissue reaction to Aroeira (Myracrodruon urundeuva) extracts: a histologic and edemogenic study |
title_sort |
evaluation of tissue reaction to aroeira (myracrodruon urundeuva) extracts: a histologic and edemogenic study |
publisher |
University of São Paulo |
series |
Journal of Applied Oral Science |
issn |
1678-7757 1678-7765 |
publishDate |
2012-08-01 |
description |
OBJECTIVES: This study evaluated subcutaneous tissue response to Aroeira (Myracrodruon urundeuva) extract employing edemogenic and histological analyses. MATERIAL AND METHODS: Test groups consisted of aqueous and ethanolic Aroeira extracts and saline (control). For edema quantification, 18 rats received an intravenous injection of Evan's Blue. After 30 min, the extracts and saline were injected on the dorsum of the rats, which were then sacrificed after 3 and 6 h. Readings were performed in a spectrophotometer. For subcutaneous implantation, 30 rats received a polyethylene tube containing the extracts on their dorsum and then they were killed after 7 and 28 days. The samples were processed for histological analysis and evaluated with a light microscope. The inflammatory infiltrate was quantified. RESULTS: There were no statistically significant differences between aqueous extract and saline groups in relation to edema quantification in the different periods (p>0.05). Ethanolic solution resulted in more edema independently of the experimental period (p<0.05). Histological analysis showed similar results on the 7-day period for the 3 groups. There was a notable reduction on inflammatory cell number for saline and aqueous extract groups at 28 days. CONCLUSION: The aqueous extract showed biocompatible properties similar to those of saline. |
topic |
Inflammation Edema Medicinal plants Anacardiaceae |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572012000400005 |
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