Candidate genes related to reproductive traits of Hereford and Braford bulls
This study emphasized the importance of using candidate genes in predicting semen quality in bulls that can be used in cow-calf production. The aim of this study was to evaluate some candidate genes related to reproductive traits in Braford and Hereford bulls. All bulls (n=188) were submitted to bre...
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Universidade Estadual de Londrina
2018-05-01
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doaj-a23d7de08fcd4ab8a5112ee2bae8c4232020-11-24T21:17:50ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592018-05-013931335135010.5433/1679-0359.2018v39n3p133517258Candidate genes related to reproductive traits of Hereford and Braford bullsSilvio Renato Oliveira Menegassi0Gabriel Ribas Pereira1Paulo Ricardo Loss Aguiar2Katiana Santos Stelmach Pereira3Celso Koetz Junior4José Braccini Neto5Vanessa Peripolli6Carolina Gabriela Becker Berlitz7Júlio Otávio Jardim Barcellos8Universidade Federal do Rio Grande do SulUniversidade do Norte do ParanáUniversidade Luterana do BrasilUniversidade Luterana do BrasilUniversidade do Norte do ParanáUniversidade Federal do Rio Grande do SulInstituto Federal CatarinenseUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulThis study emphasized the importance of using candidate genes in predicting semen quality in bulls that can be used in cow-calf production. The aim of this study was to evaluate some candidate genes related to reproductive traits in Braford and Hereford bulls. All bulls (n=188) were submitted to breeding soundness evaluations at 24, 28, 32, and 36 months of age. The microsatellite markers ILSTS002 and BMS3004 associated with the luteinizing hormone-? (LH?) gene, IDVGA-51 to leptin (LEP) gene, HEL5 and AFZ1 within the IGF-IR gene, and two SNP markers (LHR and FSHR) associated with the LHR and FSHR genes, respectively, were evaluated by the amplification of DNA products. The variation in the IDVGA-51 allele 177-185 showed polymorphic information content (PIC) associated with sperm motility and vigor traits in Hereford bulls. Hereford animals showed PIC of 0.36 to 0.75% along with expected heterozygosity (H) of 0.49 to 0.78%. Braford bulls that indicated the ILSTS002 allele 137-175 and AFZ1 allele 113-119 showed PIC associated with major and minor defects, respectively. The PIC ranged from 0.28 to 0.78%, with an expected H of 0.35 to 0.81%. AFZ1 allele 121-127 had the highest minor defects and ILSTS002 allele 125-135 showed the highest major defects from ejaculated semen in Braford bulls. In addition, IDVGA-51 allele 175 showed lower motility and vigor in Hereford bulls. The markers AFZ1 (IGF-IR) and ILSTS002 (LH?) in Braford and IDVGA-51 (LEP) in Hereford bulls were effective in verifying the reproductive traits of the bulls.http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/30750Molecular markersSingle nucleotide polymorphismsMicrosatellitesGenetic variabilityBraford and Hereford breeds. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Silvio Renato Oliveira Menegassi Gabriel Ribas Pereira Paulo Ricardo Loss Aguiar Katiana Santos Stelmach Pereira Celso Koetz Junior José Braccini Neto Vanessa Peripolli Carolina Gabriela Becker Berlitz Júlio Otávio Jardim Barcellos |
spellingShingle |
Silvio Renato Oliveira Menegassi Gabriel Ribas Pereira Paulo Ricardo Loss Aguiar Katiana Santos Stelmach Pereira Celso Koetz Junior José Braccini Neto Vanessa Peripolli Carolina Gabriela Becker Berlitz Júlio Otávio Jardim Barcellos Candidate genes related to reproductive traits of Hereford and Braford bulls Semina: Ciências Agrárias Molecular markers Single nucleotide polymorphisms Microsatellites Genetic variability Braford and Hereford breeds. |
author_facet |
Silvio Renato Oliveira Menegassi Gabriel Ribas Pereira Paulo Ricardo Loss Aguiar Katiana Santos Stelmach Pereira Celso Koetz Junior José Braccini Neto Vanessa Peripolli Carolina Gabriela Becker Berlitz Júlio Otávio Jardim Barcellos |
author_sort |
Silvio Renato Oliveira Menegassi |
title |
Candidate genes related to reproductive traits of Hereford and Braford bulls |
title_short |
Candidate genes related to reproductive traits of Hereford and Braford bulls |
title_full |
Candidate genes related to reproductive traits of Hereford and Braford bulls |
title_fullStr |
Candidate genes related to reproductive traits of Hereford and Braford bulls |
title_full_unstemmed |
Candidate genes related to reproductive traits of Hereford and Braford bulls |
title_sort |
candidate genes related to reproductive traits of hereford and braford bulls |
publisher |
Universidade Estadual de Londrina |
series |
Semina: Ciências Agrárias |
issn |
1676-546X 1679-0359 |
publishDate |
2018-05-01 |
description |
This study emphasized the importance of using candidate genes in predicting semen quality in bulls that can be used in cow-calf production. The aim of this study was to evaluate some candidate genes related to reproductive traits in Braford and Hereford bulls. All bulls (n=188) were submitted to breeding soundness evaluations at 24, 28, 32, and 36 months of age. The microsatellite markers ILSTS002 and BMS3004 associated with the luteinizing hormone-? (LH?) gene, IDVGA-51 to leptin (LEP) gene, HEL5 and AFZ1 within the IGF-IR gene, and two SNP markers (LHR and FSHR) associated with the LHR and FSHR genes, respectively, were evaluated by the amplification of DNA products. The variation in the IDVGA-51 allele 177-185 showed polymorphic information content (PIC) associated with sperm motility and vigor traits in Hereford bulls. Hereford animals showed PIC of 0.36 to 0.75% along with expected heterozygosity (H) of 0.49 to 0.78%. Braford bulls that indicated the ILSTS002 allele 137-175 and AFZ1 allele 113-119 showed PIC associated with major and minor defects, respectively. The PIC ranged from 0.28 to 0.78%, with an expected H of 0.35 to 0.81%. AFZ1 allele 121-127 had the highest minor defects and ILSTS002 allele 125-135 showed the highest major defects from ejaculated semen in Braford bulls. In addition, IDVGA-51 allele 175 showed lower motility and vigor in Hereford bulls. The markers AFZ1 (IGF-IR) and ILSTS002 (LH?) in Braford and IDVGA-51 (LEP) in Hereford bulls were effective in verifying the reproductive traits of the bulls. |
topic |
Molecular markers Single nucleotide polymorphisms Microsatellites Genetic variability Braford and Hereford breeds. |
url |
http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/30750 |
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