Increased nucleoside diphosphate kinase activity induces white spot syndrome virus infection in Litopenaeus vannamei.
Nucleoside diphosphate kinase (NDK), which has the same sequence as oncoprotein (OP) in humans, can induce nucleoside triphosphates in DNA replication by maintenance of the deoxynucleotide triphosphate (dNTP's) and is known to be regulated by viral infection in the shrimp Litopenaeus vannamei....
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doaj-a23fc460e158489896ddfd85fa90ef1d2020-11-25T02:47:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01125e017574110.1371/journal.pone.0175741Increased nucleoside diphosphate kinase activity induces white spot syndrome virus infection in Litopenaeus vannamei.Peng-Fei LiuQing-Hui LiuYin WuJie HuangNucleoside diphosphate kinase (NDK), which has the same sequence as oncoprotein (OP) in humans, can induce nucleoside triphosphates in DNA replication by maintenance of the deoxynucleotide triphosphate (dNTP's) and is known to be regulated by viral infection in the shrimp Litopenaeus vannamei. This paper describes the relationship between NDK and white spot syndrome virus (WSSV) infection. The recombinant NDK was produced by a prokaryotic expression system. WSSV copy numbers and mRNA levels of IE1 and VP28 were significantly increased in shrimp injected with recombinant NDK at 72 h after WSSV infection. After synthesizing dsRNA-NDK and confirming the efficacy of NDK silencing, we recorded the cumulative mortality of WSSV-infected shrimp injected with NDK and dsRNA-NDK. A comparison between the results demonstrated that silencing NDK delayed the death of shrimps. These findings indicate that NDK has an important role influencing the replication of WSSV replication in shrimp. Furthermore, NDK may have potential target as a new therapeutic strategy against WSSV infection in shrimp.http://europepmc.org/articles/PMC5432163?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Peng-Fei Liu Qing-Hui Liu Yin Wu Jie Huang |
spellingShingle |
Peng-Fei Liu Qing-Hui Liu Yin Wu Jie Huang Increased nucleoside diphosphate kinase activity induces white spot syndrome virus infection in Litopenaeus vannamei. PLoS ONE |
author_facet |
Peng-Fei Liu Qing-Hui Liu Yin Wu Jie Huang |
author_sort |
Peng-Fei Liu |
title |
Increased nucleoside diphosphate kinase activity induces white spot syndrome virus infection in Litopenaeus vannamei. |
title_short |
Increased nucleoside diphosphate kinase activity induces white spot syndrome virus infection in Litopenaeus vannamei. |
title_full |
Increased nucleoside diphosphate kinase activity induces white spot syndrome virus infection in Litopenaeus vannamei. |
title_fullStr |
Increased nucleoside diphosphate kinase activity induces white spot syndrome virus infection in Litopenaeus vannamei. |
title_full_unstemmed |
Increased nucleoside diphosphate kinase activity induces white spot syndrome virus infection in Litopenaeus vannamei. |
title_sort |
increased nucleoside diphosphate kinase activity induces white spot syndrome virus infection in litopenaeus vannamei. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2017-01-01 |
description |
Nucleoside diphosphate kinase (NDK), which has the same sequence as oncoprotein (OP) in humans, can induce nucleoside triphosphates in DNA replication by maintenance of the deoxynucleotide triphosphate (dNTP's) and is known to be regulated by viral infection in the shrimp Litopenaeus vannamei. This paper describes the relationship between NDK and white spot syndrome virus (WSSV) infection. The recombinant NDK was produced by a prokaryotic expression system. WSSV copy numbers and mRNA levels of IE1 and VP28 were significantly increased in shrimp injected with recombinant NDK at 72 h after WSSV infection. After synthesizing dsRNA-NDK and confirming the efficacy of NDK silencing, we recorded the cumulative mortality of WSSV-infected shrimp injected with NDK and dsRNA-NDK. A comparison between the results demonstrated that silencing NDK delayed the death of shrimps. These findings indicate that NDK has an important role influencing the replication of WSSV replication in shrimp. Furthermore, NDK may have potential target as a new therapeutic strategy against WSSV infection in shrimp. |
url |
http://europepmc.org/articles/PMC5432163?pdf=render |
work_keys_str_mv |
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