Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens
The genus Phytomonas comprises trypanosomatids that can parasitize a broad range of plant species. These fagellates can cause diseases in some plant families with a wide geographic distribution, which can result in great economic losses. We have demonstrated previously that Phytomonas serpens 15T, a...
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doaj-a249f03d9d1c498c880d9819c3a30ade2020-11-25T01:31:50ZengBMCBiological Research0716-97600717-62872010-01-01432233241Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpensViviane K Graça-de SouzaViviane Monteiro-GóesPatrício ManqueTatiana A.C.B SouzaPaulo R.C. CorrêaGregory A BuckAndréa R ÁvilaLucy M YamauchiPhileno Pinge-FilhoSamuel GoldenbergMarco A KriegerSueli F Yamada-OgattaThe genus Phytomonas comprises trypanosomatids that can parasitize a broad range of plant species. These fagellates can cause diseases in some plant families with a wide geographic distribution, which can result in great economic losses. We have demonstrated previously that Phytomonas serpens 15T, a tomato trypanosomatid, shares antigens with Trypanosoma cruzi, the agent of human Chagas disease. Herein, two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identify proteins of P. serpens 15T that are recognized by sera from patients with Chagas disease. After 2D-electrophoresis of whole-cell lysates, 31 peptides were selected and analyzed by tandem mass spectrometry. Twenty-eight polypeptides were identifed, resulting in 22 different putative proteins. The identifed proteins were classifed into 8 groups according to biological process, most of which were clustered into a cellular metabolic process category. These results generated a collection of proteins that can provide a starting point to obtain insights into antigenic cross reactivity among trypanosomatids and to explore P. serpens antigens as candidates for vaccine and immunologic diagnosis studies.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200011Phytomonas serpensTrypanosoma cruziantigenic cross-reactivitytrypanosomatids |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Viviane K Graça-de Souza Viviane Monteiro-Góes Patrício Manque Tatiana A.C.B Souza Paulo R.C. Corrêa Gregory A Buck Andréa R Ávila Lucy M Yamauchi Phileno Pinge-Filho Samuel Goldenberg Marco A Krieger Sueli F Yamada-Ogatta |
spellingShingle |
Viviane K Graça-de Souza Viviane Monteiro-Góes Patrício Manque Tatiana A.C.B Souza Paulo R.C. Corrêa Gregory A Buck Andréa R Ávila Lucy M Yamauchi Phileno Pinge-Filho Samuel Goldenberg Marco A Krieger Sueli F Yamada-Ogatta Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens Biological Research Phytomonas serpens Trypanosoma cruzi antigenic cross-reactivity trypanosomatids |
author_facet |
Viviane K Graça-de Souza Viviane Monteiro-Góes Patrício Manque Tatiana A.C.B Souza Paulo R.C. Corrêa Gregory A Buck Andréa R Ávila Lucy M Yamauchi Phileno Pinge-Filho Samuel Goldenberg Marco A Krieger Sueli F Yamada-Ogatta |
author_sort |
Viviane K Graça-de Souza |
title |
Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens |
title_short |
Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens |
title_full |
Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens |
title_fullStr |
Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens |
title_full_unstemmed |
Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens |
title_sort |
sera of chagasic patients react with antigens from the tomato parasite phytomonas serpens |
publisher |
BMC |
series |
Biological Research |
issn |
0716-9760 0717-6287 |
publishDate |
2010-01-01 |
description |
The genus Phytomonas comprises trypanosomatids that can parasitize a broad range of plant species. These fagellates can cause diseases in some plant families with a wide geographic distribution, which can result in great economic losses. We have demonstrated previously that Phytomonas serpens 15T, a tomato trypanosomatid, shares antigens with Trypanosoma cruzi, the agent of human Chagas disease. Herein, two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identify proteins of P. serpens 15T that are recognized by sera from patients with Chagas disease. After 2D-electrophoresis of whole-cell lysates, 31 peptides were selected and analyzed by tandem mass spectrometry. Twenty-eight polypeptides were identifed, resulting in 22 different putative proteins. The identifed proteins were classifed into 8 groups according to biological process, most of which were clustered into a cellular metabolic process category. These results generated a collection of proteins that can provide a starting point to obtain insights into antigenic cross reactivity among trypanosomatids and to explore P. serpens antigens as candidates for vaccine and immunologic diagnosis studies. |
topic |
Phytomonas serpens Trypanosoma cruzi antigenic cross-reactivity trypanosomatids |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200011 |
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