Alterations in Biomarkers Associated with Sterility in Pectinophora gossypiella (Saunders) Induced by Gamma Irradiation

• Main Authors: Hanaa Mahmoud Ali, Hemat Zakaria Moustafa, Rehab Mahmoud Sayed
• Format: Article
• Language: English
• Published: Instituto de Tecnologia do Paraná (Tecpar) 2017-10-01
• Series: Brazilian Archives of Biology and Technology
• Subjects: Pectinophora gossypiella; gamma rays; RAPD-PCR; elements; DNA
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100436&lng=en&tlng=en

ABSTRACT Nowadays, radiation technology is widely used to produce changes in Biosystems. The goal of this work is to determine the variation induced male Pectinophora gossypiella in gamma-irradiated as pupae using 50Gy and 150Gy. Comparing elements composition and DNA (using RAPD-PCR) between substerile 50Gy and the sterile dose 150Gy in P. gossypiella showed variation between them. Potassium (K) was the most abundant elements in unirradiated and irradiated males followed by magnesium (Mg). The percentage of heavy metals as copper, zinc, and cadmium concurrent with K was directly proportional to the radiation dose. While the percentage of Mg, Phosphorous and calcium decreased as the radiation dose increased. The results also revealed that some extra bands appeared and others disappeared, as a result of irradiation. The appearance of extra bands may be due to the repair mechanism of the irradiation damaged DNA. The banding patterns obtained and the dendrograms drawn on the basis of presence and absence of bands revealed that 150Gy irradiated pupae are more different from the unirradiated pupae than the 50Gy irradiated pupae. It was concluded that the sterile male technique could be used as a benefit tool in controlling P. gossypiella.