Tripolyphosphate Cross-Linked Macromolecular Composites for the Growth of Shape- and Size-Controlled Apatites

Bioactive composites that enable the formation of calcium phosphates have received increased attention over the last decade, in the development of osteoconductive biomaterials for orthopaedic applications. In this work, tripolyphosphate (TPP)-cross-linked chitosan/gelatin composites (TPP-CG) were pr...

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Main Authors: Fwu-Long Mi, Chih-Kang Peng, Shu-Huei Yu, Shao-Jung Wu, Jui-Yu Wu
Format: Article
Language:English
Published: MDPI AG 2012-12-01
Series:Molecules
Subjects:
Online Access:http://www.mdpi.com/1420-3049/18/1/27
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spelling doaj-a24c99a3ae95464c8c9b980674b3c4f92020-11-24T23:31:06ZengMDPI AGMolecules1420-30492012-12-01181274010.3390/molecules18010027Tripolyphosphate Cross-Linked Macromolecular Composites for the Growth of Shape- and Size-Controlled ApatitesFwu-Long MiChih-Kang PengShu-Huei YuShao-Jung WuJui-Yu WuBioactive composites that enable the formation of calcium phosphates have received increased attention over the last decade, in the development of osteoconductive biomaterials for orthopaedic applications. In this work, tripolyphosphate (TPP)-cross-linked chitosan/gelatin composites (TPP-CG) were prepared for the growth of shape- and size-controlled calcium phosphates on/in the composites. The mineralization pattern of the composites, after soaking in the Ca(OH)2 aqueous solution, clearly demonstrated oriented, needle-like nanocrystallites of calcium phosphates in the matrix with especially high Ca/P molar ratio (3.98) as detected by energy dispersive X-ray spectroscopy (EDX) analysis. Subsequent to mineralization in a simulated body fluid (SBF), the mineralized composites showed micro-scaled spherical aggregates deposited on the surface and granule-like nanocrystallites grew in the matrix. The Ca/P molar ratio (1.72) and X-ray diffraction pattern of the nanocrystallites grown in the composites were similar to those of hydroxyapatite (HAp). Osteoblastic differentiation of ROS cells cultured on the mineralized composites allowed an enhanced expression of the chosen osteogenic marker (alkaline phosphatase, ALPase). These results indicated that the composites mineralized with micro- and nano-scaled calcium phosphates with various structural features make them attractive for bone tissue engineering applications.http://www.mdpi.com/1420-3049/18/1/27chitosangelatincompositesapatitemineralization
collection DOAJ
language English
format Article
sources DOAJ
author Fwu-Long Mi
Chih-Kang Peng
Shu-Huei Yu
Shao-Jung Wu
Jui-Yu Wu
spellingShingle Fwu-Long Mi
Chih-Kang Peng
Shu-Huei Yu
Shao-Jung Wu
Jui-Yu Wu
Tripolyphosphate Cross-Linked Macromolecular Composites for the Growth of Shape- and Size-Controlled Apatites
Molecules
chitosan
gelatin
composites
apatite
mineralization
author_facet Fwu-Long Mi
Chih-Kang Peng
Shu-Huei Yu
Shao-Jung Wu
Jui-Yu Wu
author_sort Fwu-Long Mi
title Tripolyphosphate Cross-Linked Macromolecular Composites for the Growth of Shape- and Size-Controlled Apatites
title_short Tripolyphosphate Cross-Linked Macromolecular Composites for the Growth of Shape- and Size-Controlled Apatites
title_full Tripolyphosphate Cross-Linked Macromolecular Composites for the Growth of Shape- and Size-Controlled Apatites
title_fullStr Tripolyphosphate Cross-Linked Macromolecular Composites for the Growth of Shape- and Size-Controlled Apatites
title_full_unstemmed Tripolyphosphate Cross-Linked Macromolecular Composites for the Growth of Shape- and Size-Controlled Apatites
title_sort tripolyphosphate cross-linked macromolecular composites for the growth of shape- and size-controlled apatites
publisher MDPI AG
series Molecules
issn 1420-3049
publishDate 2012-12-01
description Bioactive composites that enable the formation of calcium phosphates have received increased attention over the last decade, in the development of osteoconductive biomaterials for orthopaedic applications. In this work, tripolyphosphate (TPP)-cross-linked chitosan/gelatin composites (TPP-CG) were prepared for the growth of shape- and size-controlled calcium phosphates on/in the composites. The mineralization pattern of the composites, after soaking in the Ca(OH)2 aqueous solution, clearly demonstrated oriented, needle-like nanocrystallites of calcium phosphates in the matrix with especially high Ca/P molar ratio (3.98) as detected by energy dispersive X-ray spectroscopy (EDX) analysis. Subsequent to mineralization in a simulated body fluid (SBF), the mineralized composites showed micro-scaled spherical aggregates deposited on the surface and granule-like nanocrystallites grew in the matrix. The Ca/P molar ratio (1.72) and X-ray diffraction pattern of the nanocrystallites grown in the composites were similar to those of hydroxyapatite (HAp). Osteoblastic differentiation of ROS cells cultured on the mineralized composites allowed an enhanced expression of the chosen osteogenic marker (alkaline phosphatase, ALPase). These results indicated that the composites mineralized with micro- and nano-scaled calcium phosphates with various structural features make them attractive for bone tissue engineering applications.
topic chitosan
gelatin
composites
apatite
mineralization
url http://www.mdpi.com/1420-3049/18/1/27
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AT chihkangpeng tripolyphosphatecrosslinkedmacromolecularcompositesforthegrowthofshapeandsizecontrolledapatites
AT shuhueiyu tripolyphosphatecrosslinkedmacromolecularcompositesforthegrowthofshapeandsizecontrolledapatites
AT shaojungwu tripolyphosphatecrosslinkedmacromolecularcompositesforthegrowthofshapeandsizecontrolledapatites
AT juiyuwu tripolyphosphatecrosslinkedmacromolecularcompositesforthegrowthofshapeandsizecontrolledapatites
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