Tripolyphosphate Cross-Linked Macromolecular Composites for the Growth of Shape- and Size-Controlled Apatites
Bioactive composites that enable the formation of calcium phosphates have received increased attention over the last decade, in the development of osteoconductive biomaterials for orthopaedic applications. In this work, tripolyphosphate (TPP)-cross-linked chitosan/gelatin composites (TPP-CG) were pr...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2012-12-01
|
Series: | Molecules |
Subjects: | |
Online Access: | http://www.mdpi.com/1420-3049/18/1/27 |
id |
doaj-a24c99a3ae95464c8c9b980674b3c4f9 |
---|---|
record_format |
Article |
spelling |
doaj-a24c99a3ae95464c8c9b980674b3c4f92020-11-24T23:31:06ZengMDPI AGMolecules1420-30492012-12-01181274010.3390/molecules18010027Tripolyphosphate Cross-Linked Macromolecular Composites for the Growth of Shape- and Size-Controlled ApatitesFwu-Long MiChih-Kang PengShu-Huei YuShao-Jung WuJui-Yu WuBioactive composites that enable the formation of calcium phosphates have received increased attention over the last decade, in the development of osteoconductive biomaterials for orthopaedic applications. In this work, tripolyphosphate (TPP)-cross-linked chitosan/gelatin composites (TPP-CG) were prepared for the growth of shape- and size-controlled calcium phosphates on/in the composites. The mineralization pattern of the composites, after soaking in the Ca(OH)2 aqueous solution, clearly demonstrated oriented, needle-like nanocrystallites of calcium phosphates in the matrix with especially high Ca/P molar ratio (3.98) as detected by energy dispersive X-ray spectroscopy (EDX) analysis. Subsequent to mineralization in a simulated body fluid (SBF), the mineralized composites showed micro-scaled spherical aggregates deposited on the surface and granule-like nanocrystallites grew in the matrix. The Ca/P molar ratio (1.72) and X-ray diffraction pattern of the nanocrystallites grown in the composites were similar to those of hydroxyapatite (HAp). Osteoblastic differentiation of ROS cells cultured on the mineralized composites allowed an enhanced expression of the chosen osteogenic marker (alkaline phosphatase, ALPase). These results indicated that the composites mineralized with micro- and nano-scaled calcium phosphates with various structural features make them attractive for bone tissue engineering applications.http://www.mdpi.com/1420-3049/18/1/27chitosangelatincompositesapatitemineralization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fwu-Long Mi Chih-Kang Peng Shu-Huei Yu Shao-Jung Wu Jui-Yu Wu |
spellingShingle |
Fwu-Long Mi Chih-Kang Peng Shu-Huei Yu Shao-Jung Wu Jui-Yu Wu Tripolyphosphate Cross-Linked Macromolecular Composites for the Growth of Shape- and Size-Controlled Apatites Molecules chitosan gelatin composites apatite mineralization |
author_facet |
Fwu-Long Mi Chih-Kang Peng Shu-Huei Yu Shao-Jung Wu Jui-Yu Wu |
author_sort |
Fwu-Long Mi |
title |
Tripolyphosphate Cross-Linked Macromolecular Composites for the Growth of Shape- and Size-Controlled Apatites |
title_short |
Tripolyphosphate Cross-Linked Macromolecular Composites for the Growth of Shape- and Size-Controlled Apatites |
title_full |
Tripolyphosphate Cross-Linked Macromolecular Composites for the Growth of Shape- and Size-Controlled Apatites |
title_fullStr |
Tripolyphosphate Cross-Linked Macromolecular Composites for the Growth of Shape- and Size-Controlled Apatites |
title_full_unstemmed |
Tripolyphosphate Cross-Linked Macromolecular Composites for the Growth of Shape- and Size-Controlled Apatites |
title_sort |
tripolyphosphate cross-linked macromolecular composites for the growth of shape- and size-controlled apatites |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2012-12-01 |
description |
Bioactive composites that enable the formation of calcium phosphates have received increased attention over the last decade, in the development of osteoconductive biomaterials for orthopaedic applications. In this work, tripolyphosphate (TPP)-cross-linked chitosan/gelatin composites (TPP-CG) were prepared for the growth of shape- and size-controlled calcium phosphates on/in the composites. The mineralization pattern of the composites, after soaking in the Ca(OH)2 aqueous solution, clearly demonstrated oriented, needle-like nanocrystallites of calcium phosphates in the matrix with especially high Ca/P molar ratio (3.98) as detected by energy dispersive X-ray spectroscopy (EDX) analysis. Subsequent to mineralization in a simulated body fluid (SBF), the mineralized composites showed micro-scaled spherical aggregates deposited on the surface and granule-like nanocrystallites grew in the matrix. The Ca/P molar ratio (1.72) and X-ray diffraction pattern of the nanocrystallites grown in the composites were similar to those of hydroxyapatite (HAp). Osteoblastic differentiation of ROS cells cultured on the mineralized composites allowed an enhanced expression of the chosen osteogenic marker (alkaline phosphatase, ALPase). These results indicated that the composites mineralized with micro- and nano-scaled calcium phosphates with various structural features make them attractive for bone tissue engineering applications. |
topic |
chitosan gelatin composites apatite mineralization |
url |
http://www.mdpi.com/1420-3049/18/1/27 |
work_keys_str_mv |
AT fwulongmi tripolyphosphatecrosslinkedmacromolecularcompositesforthegrowthofshapeandsizecontrolledapatites AT chihkangpeng tripolyphosphatecrosslinkedmacromolecularcompositesforthegrowthofshapeandsizecontrolledapatites AT shuhueiyu tripolyphosphatecrosslinkedmacromolecularcompositesforthegrowthofshapeandsizecontrolledapatites AT shaojungwu tripolyphosphatecrosslinkedmacromolecularcompositesforthegrowthofshapeandsizecontrolledapatites AT juiyuwu tripolyphosphatecrosslinkedmacromolecularcompositesforthegrowthofshapeandsizecontrolledapatites |
_version_ |
1725538909203464192 |