Newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eye
Summary: Newts utilize their unique genes to restore missing parts by strategic regulation of conserved signaling pathways. Lack of genetic tools poses challenges to determine the function of such genes. Therefore, we used the Drosophila eye model to demonstrate the potential of 5 unique newt (Notop...
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doaj-a259047234e242faa1a81c4343464d252021-10-09T04:40:50ZengElsevieriScience2589-00422021-10-012410103166Newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eyeAbijeet Singh Mehta0Prajakta Deshpande1Anuradha Venkatakrishnan Chimata2Panagiotis A. Tsonis3Amit Singh4Department of Biology, University of Dayton, Dayton, OH 45469, USADepartment of Biology, University of Dayton, Dayton, OH 45469, USADepartment of Biology, University of Dayton, Dayton, OH 45469, USADepartment of Biology, University of Dayton, Dayton, OH 45469, USADepartment of Biology, University of Dayton, Dayton, OH 45469, USA; Premedical Program, University of Dayton, Dayton, USA; Center for Tissue Regeneration and Engineering at Dayton (TREND), University of Dayton, Dayton, USA; The Integrative Science and Engineering Center, University of Dayton, Dayton, OH 45469, USA; Center for Genomic Advocacy (TCGA), Indiana State University, Terre Haute, IN, USA; Corresponding authorSummary: Newts utilize their unique genes to restore missing parts by strategic regulation of conserved signaling pathways. Lack of genetic tools poses challenges to determine the function of such genes. Therefore, we used the Drosophila eye model to demonstrate the potential of 5 unique newt (Notophthalmus viridescens) gene(s), viropana1-viropana5 (vna1-vna5), which were ectopically expressed in L2 mutant and GMR-hid, GMR-GAL4 eye. L2 exhibits the loss of ventral half of early eye and head involution defective (hid) triggers cell-death during later eye development. Surprisingly, newt genes significantly restore missing photoreceptor cells both in L2 and GMR>hid background by upregulating cell-proliferation and blocking cell-death, regulating evolutionarily conserved Wingless (Wg)/Wnt signaling pathway and exhibit non-cell-autonomous rescues. Further, Wg/Wnt signaling acts downstream of newt genes. Our data highlights that unique newt proteins can regulate conserved pathways to trigger a robust restoration of missing photoreceptor cells in Drosophila eye model with weak restoration capability.http://www.sciencedirect.com/science/article/pii/S2589004221011342Biological sciencesCell biologyDevelopmental biology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Abijeet Singh Mehta Prajakta Deshpande Anuradha Venkatakrishnan Chimata Panagiotis A. Tsonis Amit Singh |
spellingShingle |
Abijeet Singh Mehta Prajakta Deshpande Anuradha Venkatakrishnan Chimata Panagiotis A. Tsonis Amit Singh Newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eye iScience Biological sciences Cell biology Developmental biology |
author_facet |
Abijeet Singh Mehta Prajakta Deshpande Anuradha Venkatakrishnan Chimata Panagiotis A. Tsonis Amit Singh |
author_sort |
Abijeet Singh Mehta |
title |
Newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eye |
title_short |
Newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eye |
title_full |
Newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eye |
title_fullStr |
Newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eye |
title_full_unstemmed |
Newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eye |
title_sort |
newt regeneration genes regulate wingless signaling to restore patterning in drosophila eye |
publisher |
Elsevier |
series |
iScience |
issn |
2589-0042 |
publishDate |
2021-10-01 |
description |
Summary: Newts utilize their unique genes to restore missing parts by strategic regulation of conserved signaling pathways. Lack of genetic tools poses challenges to determine the function of such genes. Therefore, we used the Drosophila eye model to demonstrate the potential of 5 unique newt (Notophthalmus viridescens) gene(s), viropana1-viropana5 (vna1-vna5), which were ectopically expressed in L2 mutant and GMR-hid, GMR-GAL4 eye. L2 exhibits the loss of ventral half of early eye and head involution defective (hid) triggers cell-death during later eye development. Surprisingly, newt genes significantly restore missing photoreceptor cells both in L2 and GMR>hid background by upregulating cell-proliferation and blocking cell-death, regulating evolutionarily conserved Wingless (Wg)/Wnt signaling pathway and exhibit non-cell-autonomous rescues. Further, Wg/Wnt signaling acts downstream of newt genes. Our data highlights that unique newt proteins can regulate conserved pathways to trigger a robust restoration of missing photoreceptor cells in Drosophila eye model with weak restoration capability. |
topic |
Biological sciences Cell biology Developmental biology |
url |
http://www.sciencedirect.com/science/article/pii/S2589004221011342 |
work_keys_str_mv |
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