Human serum albumin and oxidative stress in preeclamptic women and the mechanism of albumin for stress reduction
Aims: The present study to address one of the mechanisms in preeclampsia, examined whether levels of oxidative stress, human serum albumin, and endothelial function correlate in pregnant women and whether human serum albumin reduces levels of superoxide produced by NADPH oxidase activation in the hu...
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doaj-a27024527c7d4fb59ed5a27389d12afe2020-11-24T20:42:53ZengElsevierHeliyon2405-84402017-08-013810.1016/j.heliyon.2017.e00369Human serum albumin and oxidative stress in preeclamptic women and the mechanism of albumin for stress reductionHiroyuki Kinoshita0Kazushi Watanabe1Toshiharu Azma2Guo-Gang Feng3Takahiko Akahori4Hisaki Hayashi5Motohiko Sato6Yoshihiro Fujiwara7Akihiko Wakatsuki8Departments of Anesthesiology, Aichi Medical University School of Medicine, 1-1 Yazako Karimata, Nagakute, Aichi 480-1195, JapanObstetrics and Gynecology, Aichi Medical University School of Medicine, 1-1 Yazako Karimata, Nagakute, Aichi 480-1195, JapanDepartment of Anesthesiology & Pain Medicine, Kohnodai Hospital, National Center for Global Health and Medicine, Ichikawa, Chiba 272-8516, JapanPharmacology, Aichi Medical University School of Medicine, 1-1 Yazako Karimata, Nagakute, Aichi 480-1195, JapanDepartments of Anesthesiology, Aichi Medical University School of Medicine, 1-1 Yazako Karimata, Nagakute, Aichi 480-1195, JapanPhysiology, Aichi Medical University School of Medicine, 1-1 Yazako Karimata, Nagakute, Aichi 480-1195, JapanPhysiology, Aichi Medical University School of Medicine, 1-1 Yazako Karimata, Nagakute, Aichi 480-1195, JapanDepartments of Anesthesiology, Aichi Medical University School of Medicine, 1-1 Yazako Karimata, Nagakute, Aichi 480-1195, JapanObstetrics and Gynecology, Aichi Medical University School of Medicine, 1-1 Yazako Karimata, Nagakute, Aichi 480-1195, JapanAims: The present study to address one of the mechanisms in preeclampsia, examined whether levels of oxidative stress, human serum albumin, and endothelial function correlate in pregnant women and whether human serum albumin reduces levels of superoxide produced by NADPH oxidase activation in the human vascular smooth muscle cells. Materials and methods: Pregnant women with (Preeclampsia group, n = 33) and without preeclampsia (Normal group, n = 37) were recruited to determine levels of reactive oxygen species (serum diacron-reactive oxygen metabolite [d-ROM]), and the flow-mediated dilation (FMD). Human coronary arterial smooth muscle cells or omental arteries were subjected to evaluate isometric force recordings, levels of superoxide, western immunoblotting, and immunohistochemistry. The superoxide scavenging assay was also performed in a cell-free system. Key findings: Women in the preeclampsia group demonstrated lower FMD and higher serum d-ROM values than those in the normal group. There were the inverse correlations between serum levels of d-ROM and the degree of FMD and between serum levels of albumin and those of d-ROM. D-glucose reduced the levcromakalim-induced dilation of human omental arteries, and it increased levels of superoxide and the recruitment of the NADPH oxidase subunit p47phox in human coronary arterial smooth muscle cells. Human serum albumin (0.05 to 0.5 g/dL) prevented these alterations whereas it exerted no superoxide scavenging effect. Significance: Serum albumin relates to oxidative stress inversely, but to the endothelial function positively, in pregnant women. Human serum albumin appears to reduce oxidative stress via NADPH oxidase inhibition in the human vascular smooth muscle, indicating that the serum level may be a critical determinant of vascular oxidative stress in some human diseases.http://www.sciencedirect.com/science/article/pii/S2405844017308241MedicineReproductive medicineSystems biologyCell biologyEndocrinologyMetabolismCardiologyPathologyPhysiology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hiroyuki Kinoshita Kazushi Watanabe Toshiharu Azma Guo-Gang Feng Takahiko Akahori Hisaki Hayashi Motohiko Sato Yoshihiro Fujiwara Akihiko Wakatsuki |
spellingShingle |
Hiroyuki Kinoshita Kazushi Watanabe Toshiharu Azma Guo-Gang Feng Takahiko Akahori Hisaki Hayashi Motohiko Sato Yoshihiro Fujiwara Akihiko Wakatsuki Human serum albumin and oxidative stress in preeclamptic women and the mechanism of albumin for stress reduction Heliyon Medicine Reproductive medicine Systems biology Cell biology Endocrinology Metabolism Cardiology Pathology Physiology |
author_facet |
Hiroyuki Kinoshita Kazushi Watanabe Toshiharu Azma Guo-Gang Feng Takahiko Akahori Hisaki Hayashi Motohiko Sato Yoshihiro Fujiwara Akihiko Wakatsuki |
author_sort |
Hiroyuki Kinoshita |
title |
Human serum albumin and oxidative stress in preeclamptic women and the mechanism of albumin for stress reduction |
title_short |
Human serum albumin and oxidative stress in preeclamptic women and the mechanism of albumin for stress reduction |
title_full |
Human serum albumin and oxidative stress in preeclamptic women and the mechanism of albumin for stress reduction |
title_fullStr |
Human serum albumin and oxidative stress in preeclamptic women and the mechanism of albumin for stress reduction |
title_full_unstemmed |
Human serum albumin and oxidative stress in preeclamptic women and the mechanism of albumin for stress reduction |
title_sort |
human serum albumin and oxidative stress in preeclamptic women and the mechanism of albumin for stress reduction |
publisher |
Elsevier |
series |
Heliyon |
issn |
2405-8440 |
publishDate |
2017-08-01 |
description |
Aims: The present study to address one of the mechanisms in preeclampsia, examined whether levels of oxidative stress, human serum albumin, and endothelial function correlate in pregnant women and whether human serum albumin reduces levels of superoxide produced by NADPH oxidase activation in the human vascular smooth muscle cells.
Materials and methods: Pregnant women with (Preeclampsia group, n = 33) and without preeclampsia (Normal group, n = 37) were recruited to determine levels of reactive oxygen species (serum diacron-reactive oxygen metabolite [d-ROM]), and the flow-mediated dilation (FMD). Human coronary arterial smooth muscle cells or omental arteries were subjected to evaluate isometric force recordings, levels of superoxide, western immunoblotting, and immunohistochemistry. The superoxide scavenging assay was also performed in a cell-free system.
Key findings: Women in the preeclampsia group demonstrated lower FMD and higher serum d-ROM values than those in the normal group. There were the inverse correlations between serum levels of d-ROM and the degree of FMD and between serum levels of albumin and those of d-ROM. D-glucose reduced the levcromakalim-induced dilation of human omental arteries, and it increased levels of superoxide and the recruitment of the NADPH oxidase subunit p47phox in human coronary arterial smooth muscle cells. Human serum albumin (0.05 to 0.5 g/dL) prevented these alterations whereas it exerted no superoxide scavenging effect.
Significance: Serum albumin relates to oxidative stress inversely, but to the endothelial function positively, in pregnant women. Human serum albumin appears to reduce oxidative stress via NADPH oxidase inhibition in the human vascular smooth muscle, indicating that the serum level may be a critical determinant of vascular oxidative stress in some human diseases. |
topic |
Medicine Reproductive medicine Systems biology Cell biology Endocrinology Metabolism Cardiology Pathology Physiology |
url |
http://www.sciencedirect.com/science/article/pii/S2405844017308241 |
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