A Multiparametric Study of Internalization of Fullerenol C<sub>60</sub>(OH)<sub>36</sub> Nanoparticles into Peripheral Blood Mononuclear Cells: Cytotoxicity in Oxidative Stress Induced by Ionizing Radiation
The aim of this study was to investigate the uptake and accumulation of fullerenol C<sub>60</sub>(OH)<sub>36</sub> into peripheral blood mononuclear cells (PBMCs). Some additional studies were also performed: measurement of fullerenol nanoparticle size, zeta potential, and th...
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doaj-a2718bb91c774077a9021ab4673166ee2020-11-25T01:29:03ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-03-01217228110.3390/ijms21072281ijms21072281A Multiparametric Study of Internalization of Fullerenol C<sub>60</sub>(OH)<sub>36</sub> Nanoparticles into Peripheral Blood Mononuclear Cells: Cytotoxicity in Oxidative Stress Induced by Ionizing RadiationAnna Lichota0Ireneusz Piwoński1Sylwia Michlewska2Anita Krokosz3Department of Molecular Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandDepartment of Materials Technology and Chemistry, Faculty of Chemistry, University of Lodz, 90-236 Lodz, PolandLaboratory of Electron Microscopy, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandDepartment of Biophysics of Environmental Pollution, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandThe aim of this study was to investigate the uptake and accumulation of fullerenol C<sub>60</sub>(OH)<sub>36</sub> into peripheral blood mononuclear cells (PBMCs). Some additional studies were also performed: measurement of fullerenol nanoparticle size, zeta potential, and the influence of fullerenol on the ionizing radiation-induced damage to PMBCs. Fullerenol C<sub>60</sub>(OH)<sub>36</sub> demonstrated an ability to accumulate in PBMCs. The accumulation of fullerenol in those cells did not have a significant effect on cell survival, nor on the distribution of phosphatidylserine in the plasma membrane. However, fullerenol-induced depolarization of the mitochondrial membrane proportional to the compound level in the medium was observed. Results also indicated that increased fullerenol level in the medium was associated with its enhanced transport into cells, corresponding to its influence on the mitochondrial membrane. The obtained results clearly showed the ability of C<sub>60</sub>(OH)<sub>36 </sub>to enter cells and its effect on PBMC mitochondrial membrane potential. However, we did not observe radioprotective properties of fullerenol under the conditions used in our study.https://www.mdpi.com/1422-0067/21/7/2281fullerenol uptake, accumulation, ionizing radiation, mitochondrial membrane potential |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anna Lichota Ireneusz Piwoński Sylwia Michlewska Anita Krokosz |
spellingShingle |
Anna Lichota Ireneusz Piwoński Sylwia Michlewska Anita Krokosz A Multiparametric Study of Internalization of Fullerenol C<sub>60</sub>(OH)<sub>36</sub> Nanoparticles into Peripheral Blood Mononuclear Cells: Cytotoxicity in Oxidative Stress Induced by Ionizing Radiation International Journal of Molecular Sciences fullerenol uptake, accumulation, ionizing radiation, mitochondrial membrane potential |
author_facet |
Anna Lichota Ireneusz Piwoński Sylwia Michlewska Anita Krokosz |
author_sort |
Anna Lichota |
title |
A Multiparametric Study of Internalization of Fullerenol C<sub>60</sub>(OH)<sub>36</sub> Nanoparticles into Peripheral Blood Mononuclear Cells: Cytotoxicity in Oxidative Stress Induced by Ionizing Radiation |
title_short |
A Multiparametric Study of Internalization of Fullerenol C<sub>60</sub>(OH)<sub>36</sub> Nanoparticles into Peripheral Blood Mononuclear Cells: Cytotoxicity in Oxidative Stress Induced by Ionizing Radiation |
title_full |
A Multiparametric Study of Internalization of Fullerenol C<sub>60</sub>(OH)<sub>36</sub> Nanoparticles into Peripheral Blood Mononuclear Cells: Cytotoxicity in Oxidative Stress Induced by Ionizing Radiation |
title_fullStr |
A Multiparametric Study of Internalization of Fullerenol C<sub>60</sub>(OH)<sub>36</sub> Nanoparticles into Peripheral Blood Mononuclear Cells: Cytotoxicity in Oxidative Stress Induced by Ionizing Radiation |
title_full_unstemmed |
A Multiparametric Study of Internalization of Fullerenol C<sub>60</sub>(OH)<sub>36</sub> Nanoparticles into Peripheral Blood Mononuclear Cells: Cytotoxicity in Oxidative Stress Induced by Ionizing Radiation |
title_sort |
multiparametric study of internalization of fullerenol c<sub>60</sub>(oh)<sub>36</sub> nanoparticles into peripheral blood mononuclear cells: cytotoxicity in oxidative stress induced by ionizing radiation |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2020-03-01 |
description |
The aim of this study was to investigate the uptake and accumulation of fullerenol C<sub>60</sub>(OH)<sub>36</sub> into peripheral blood mononuclear cells (PBMCs). Some additional studies were also performed: measurement of fullerenol nanoparticle size, zeta potential, and the influence of fullerenol on the ionizing radiation-induced damage to PMBCs. Fullerenol C<sub>60</sub>(OH)<sub>36</sub> demonstrated an ability to accumulate in PBMCs. The accumulation of fullerenol in those cells did not have a significant effect on cell survival, nor on the distribution of phosphatidylserine in the plasma membrane. However, fullerenol-induced depolarization of the mitochondrial membrane proportional to the compound level in the medium was observed. Results also indicated that increased fullerenol level in the medium was associated with its enhanced transport into cells, corresponding to its influence on the mitochondrial membrane. The obtained results clearly showed the ability of C<sub>60</sub>(OH)<sub>36 </sub>to enter cells and its effect on PBMC mitochondrial membrane potential. However, we did not observe radioprotective properties of fullerenol under the conditions used in our study. |
topic |
fullerenol uptake, accumulation, ionizing radiation, mitochondrial membrane potential |
url |
https://www.mdpi.com/1422-0067/21/7/2281 |
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