Diagnostic Utility of Heat Stable Alkaline Phosphatase in Hypertensive Disorders of Pregnancy
Background: Hypertensive disorders in pregnancy (HDP) complicate 3-10% of all pregnancies. Though there are several biochemical parameters which aid in predicting hypertension of pregnancy, human placental alkaline phosphatase (PLAP), synthesized in placenta during pregnancy by placental syncyt...
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doaj-a2ad62d3501c46db9859bc7d66fe97e42020-11-25T02:58:57ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2014-11-01811CC10CC1310.7860/JCDR/2014/10895.5084Diagnostic Utility of Heat Stable Alkaline Phosphatase in Hypertensive Disorders of PregnancyReeta Rajagambeeram0Srinivasan Abu Raghavan1Seethesh Ghosh2Sharbari Basu3Ramesh Ramasamy4Sathish Babu Murugaiyan5Assistant Professor, Department of Biochemistry, Mahatma Gandhi Medical College & Research Institute, Pondicherry, Tamil Nadu, India.Professor, Department of Biochemistry, Mahatma Gandhi Medical College & Research Institute, Pondicherry, Tamil Nadu, India.Professor, Depratment of Obstetrics and Gynaecology, Mahatma Gandhi Medical College & Research Institute, Pondicherry, Tamil Nadu, India.Professor, Department of Biochemistry, Mahatma Gandhi Medical College & Research Institute, Pondicherry, Tamil Nadu, India.Professor, Department of Biochemistry, Mahatma Gandhi Medical College & Research Institute, Pondicherry, Tamil Nadu, India.Assistant Professor, Department of Biochemistry, Mahatma Gandhi Medical College & Research Institute, Pondicherry, Tamil Nadu, India.Background: Hypertensive disorders in pregnancy (HDP) complicate 3-10% of all pregnancies. Though there are several biochemical parameters which aid in predicting hypertension of pregnancy, human placental alkaline phosphatase (PLAP), synthesized in placenta during pregnancy by placental syncytiotrophoblast, assumes diagnostic relevance. The purpose of this study was to compare the total alkaline phosphatase (ALP) and heat stable placental alkaline phosphatase (PLAP) levels in the serum of normotensive and hypertensive disorders of pregnancy and to evaluate the clinical utility of ALP and PLAP as a reliable, sensitive, specific and economical biochemical marker of HDP. Materials and Methods: This was a case control study, carried out on pregnant women with hypertension, of south Indian population. Study included pregnant women, 60 patients with hypertension and 60 controls. Biochemical assays were carried out by the IFCC approved procedures based on spectrophotometric method and using fully automated random access chemistry analyser. Data was compared by using student t-test. ROC was drawn to find out optimum cut off for ALP, PLAP and PLAP/ALP ratio in HDP. Pearson’s correlation was performed to ascertain the association among markers. Results: Serum total ALP, PLAP and PLAP/ALP ratio levels were significantly higher in hypertensive pregnant women when compared to controls (p<0.05). There was significant correlation among ALP, PLAP and DBP. ROC analysis of ALP (169.5), PLAP (69) and PLAP/ALP (0.44) ratios showed optimum cut-offs in diagnosis of hypertension in pregnancy. Conclusion: Serum heat stable ALP isoenzyme and PLAP/ ALP ratio could be useful adjuvant markers in diagnosis of HDP in association with other relevant and economically viable biochemical tests.https://jcdr.net/articles/PDF/5084/10895_CE(Ra)_F(Sh)_PF1(NJ_Sh)_PFA(Sh).pdfheat stable alkaline phosphatasealkaline phosphatasehypertensive disorders of pregnancyreceiver operating characteristic curve |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Reeta Rajagambeeram Srinivasan Abu Raghavan Seethesh Ghosh Sharbari Basu Ramesh Ramasamy Sathish Babu Murugaiyan |
spellingShingle |
Reeta Rajagambeeram Srinivasan Abu Raghavan Seethesh Ghosh Sharbari Basu Ramesh Ramasamy Sathish Babu Murugaiyan Diagnostic Utility of Heat Stable Alkaline Phosphatase in Hypertensive Disorders of Pregnancy Journal of Clinical and Diagnostic Research heat stable alkaline phosphatase alkaline phosphatase hypertensive disorders of pregnancy receiver operating characteristic curve |
author_facet |
Reeta Rajagambeeram Srinivasan Abu Raghavan Seethesh Ghosh Sharbari Basu Ramesh Ramasamy Sathish Babu Murugaiyan |
author_sort |
Reeta Rajagambeeram |
title |
Diagnostic Utility of Heat Stable Alkaline Phosphatase in Hypertensive Disorders of Pregnancy |
title_short |
Diagnostic Utility of Heat Stable Alkaline Phosphatase in Hypertensive Disorders of Pregnancy |
title_full |
Diagnostic Utility of Heat Stable Alkaline Phosphatase in Hypertensive Disorders of Pregnancy |
title_fullStr |
Diagnostic Utility of Heat Stable Alkaline Phosphatase in Hypertensive Disorders of Pregnancy |
title_full_unstemmed |
Diagnostic Utility of Heat Stable Alkaline Phosphatase in Hypertensive Disorders of Pregnancy |
title_sort |
diagnostic utility of heat stable alkaline phosphatase in hypertensive disorders of pregnancy |
publisher |
JCDR Research and Publications Private Limited |
series |
Journal of Clinical and Diagnostic Research |
issn |
2249-782X 0973-709X |
publishDate |
2014-11-01 |
description |
Background: Hypertensive disorders in pregnancy (HDP)
complicate 3-10% of all pregnancies. Though there are several
biochemical parameters which aid in predicting hypertension
of pregnancy, human placental alkaline phosphatase (PLAP),
synthesized in placenta during pregnancy by placental
syncytiotrophoblast, assumes diagnostic relevance. The purpose
of this study was to compare the total alkaline phosphatase
(ALP) and heat stable placental alkaline phosphatase (PLAP)
levels in the serum of normotensive and hypertensive disorders
of pregnancy and to evaluate the clinical utility of ALP and PLAP
as a reliable, sensitive, specific and economical biochemical
marker of HDP.
Materials and Methods: This was a case control study, carried
out on pregnant women with hypertension, of south Indian
population.
Study included pregnant women, 60 patients with hypertension
and 60 controls. Biochemical assays were carried out by the
IFCC approved procedures based on spectrophotometric
method and using fully automated random access chemistry
analyser.
Data was compared by using student t-test. ROC was drawn
to find out optimum cut off for ALP, PLAP and PLAP/ALP ratio
in HDP. Pearson’s correlation was performed to ascertain the
association among markers.
Results: Serum total ALP, PLAP and PLAP/ALP ratio levels
were significantly higher in hypertensive pregnant women when
compared to controls (p<0.05). There was significant correlation
among ALP, PLAP and DBP. ROC analysis of ALP (169.5), PLAP
(69) and PLAP/ALP (0.44) ratios showed optimum cut-offs in
diagnosis of hypertension in pregnancy.
Conclusion: Serum heat stable ALP isoenzyme and PLAP/
ALP ratio could be useful adjuvant markers in diagnosis of
HDP in association with other relevant and economically viable
biochemical tests. |
topic |
heat stable alkaline phosphatase alkaline phosphatase hypertensive disorders of pregnancy receiver operating characteristic curve |
url |
https://jcdr.net/articles/PDF/5084/10895_CE(Ra)_F(Sh)_PF1(NJ_Sh)_PFA(Sh).pdf |
work_keys_str_mv |
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