Detection of Pig Cells Harboring Porcine Endogenous Retroviruses in Non-Human Primate Bladder After Renal Xenotransplantation
Pigs are used as potential donor animals for xenotransplantation. However, porcine endogenous retrovirus (PERV), shown to infect both human and non-human primate (NHP) cells in vitro, presents a risk of transmission to humans in xenotransplantation. In this study, we analyzed PERV transmission in va...
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doaj-a2bd5176c9b34d42affb65a48b0bad3a2020-11-24T20:42:49ZengMDPI AGViruses1999-49152019-08-0111980110.3390/v11090801v11090801Detection of Pig Cells Harboring Porcine Endogenous Retroviruses in Non-Human Primate Bladder After Renal XenotransplantationYoonki Heo0Yeondong Cho1Keon Bong Oh2Ki Hoon Park3Hansam Cho4Hanul Choi5Minjee Kim6Ik Jin Yun7Hee Jung Lee8Young Bong Kim9Department of Biomedical Science and Engineering, Konkuk University, Seoul 05029, KoreaDepartment of Biomedical Science and Engineering, Konkuk University, Seoul 05029, KoreaAnimal Biotechnology Division, National Institute of Animal Science, RDA, Jeonju, Jeollabuk-do 54875, KoreaDepartment of Biomedical Science and Engineering, Konkuk University, Seoul 05029, KoreaDepartment of Biomedical Science and Engineering, Konkuk University, Seoul 05029, KoreaDepartment of Biomedical Science and Engineering, Konkuk University, Seoul 05029, KoreaDepartment of Biomedical Science and Engineering, Konkuk University, Seoul 05029, KoreaDepartment of Surgery, Konkuk University Medical Center, Konkuk University School of Medicine, Seoul 05029, KoreaDepartment of Biomedical Science and Engineering, Konkuk University, Seoul 05029, KoreaDepartment of Biomedical Science and Engineering, Konkuk University, Seoul 05029, KoreaPigs are used as potential donor animals for xenotransplantation. However, porcine endogenous retrovirus (PERV), shown to infect both human and non-human primate (NHP) cells in vitro, presents a risk of transmission to humans in xenotransplantation. In this study, we analyzed PERV transmission in various organs after pig-to-NHP xenotransplantation. We utilized pig-to-NHP xenotransplant tissue samples obtained using two types of transgenic pigs from the National Institute of Animal Science (NIAS, Republic of Korea), and examined them for the existence of PERV genes in different organs via PCR and RT-PCR with specific primers. To determine PERV insertion into chromosomes, inverse PCR using PERV long terminal repeat (LTR) region-specific primers was conducted. The PERV gene was not detected in NHP organs in cardiac xenotransplantation but detected in NHP bladders in renal xenotransplantation. The insertion experiment confirmed that PERVs originate from porcine donor cells rather than integrated provirus in the NHP chromosome. We also demonstrate the presence of pig cells in the NHP bladder after renal xenotransplantation using specific-porcine mitochondrial DNA gene PCR. The PERV sequence was detected in the bladder of NHPs after renal xenotransplantation by porcine cell-microchimerism but did not integrate into the NHP chromosome.https://www.mdpi.com/1999-4915/11/9/801pig-to-NHP xenotransplantationheart xenotransplantationkidney xenotransplantationporcine endogenous retrovirus (PERV)microchimerism |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yoonki Heo Yeondong Cho Keon Bong Oh Ki Hoon Park Hansam Cho Hanul Choi Minjee Kim Ik Jin Yun Hee Jung Lee Young Bong Kim |
spellingShingle |
Yoonki Heo Yeondong Cho Keon Bong Oh Ki Hoon Park Hansam Cho Hanul Choi Minjee Kim Ik Jin Yun Hee Jung Lee Young Bong Kim Detection of Pig Cells Harboring Porcine Endogenous Retroviruses in Non-Human Primate Bladder After Renal Xenotransplantation Viruses pig-to-NHP xenotransplantation heart xenotransplantation kidney xenotransplantation porcine endogenous retrovirus (PERV) microchimerism |
author_facet |
Yoonki Heo Yeondong Cho Keon Bong Oh Ki Hoon Park Hansam Cho Hanul Choi Minjee Kim Ik Jin Yun Hee Jung Lee Young Bong Kim |
author_sort |
Yoonki Heo |
title |
Detection of Pig Cells Harboring Porcine Endogenous Retroviruses in Non-Human Primate Bladder After Renal Xenotransplantation |
title_short |
Detection of Pig Cells Harboring Porcine Endogenous Retroviruses in Non-Human Primate Bladder After Renal Xenotransplantation |
title_full |
Detection of Pig Cells Harboring Porcine Endogenous Retroviruses in Non-Human Primate Bladder After Renal Xenotransplantation |
title_fullStr |
Detection of Pig Cells Harboring Porcine Endogenous Retroviruses in Non-Human Primate Bladder After Renal Xenotransplantation |
title_full_unstemmed |
Detection of Pig Cells Harboring Porcine Endogenous Retroviruses in Non-Human Primate Bladder After Renal Xenotransplantation |
title_sort |
detection of pig cells harboring porcine endogenous retroviruses in non-human primate bladder after renal xenotransplantation |
publisher |
MDPI AG |
series |
Viruses |
issn |
1999-4915 |
publishDate |
2019-08-01 |
description |
Pigs are used as potential donor animals for xenotransplantation. However, porcine endogenous retrovirus (PERV), shown to infect both human and non-human primate (NHP) cells in vitro, presents a risk of transmission to humans in xenotransplantation. In this study, we analyzed PERV transmission in various organs after pig-to-NHP xenotransplantation. We utilized pig-to-NHP xenotransplant tissue samples obtained using two types of transgenic pigs from the National Institute of Animal Science (NIAS, Republic of Korea), and examined them for the existence of PERV genes in different organs via PCR and RT-PCR with specific primers. To determine PERV insertion into chromosomes, inverse PCR using PERV long terminal repeat (LTR) region-specific primers was conducted. The PERV gene was not detected in NHP organs in cardiac xenotransplantation but detected in NHP bladders in renal xenotransplantation. The insertion experiment confirmed that PERVs originate from porcine donor cells rather than integrated provirus in the NHP chromosome. We also demonstrate the presence of pig cells in the NHP bladder after renal xenotransplantation using specific-porcine mitochondrial DNA gene PCR. The PERV sequence was detected in the bladder of NHPs after renal xenotransplantation by porcine cell-microchimerism but did not integrate into the NHP chromosome. |
topic |
pig-to-NHP xenotransplantation heart xenotransplantation kidney xenotransplantation porcine endogenous retrovirus (PERV) microchimerism |
url |
https://www.mdpi.com/1999-4915/11/9/801 |
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