Engineered cyclodextrin glucanotransferases from Bacillus sp. G‐825‐6 produce large‐ring cyclodextrins with high specificity
Abstract Cyclodextrin glucanotransferases (CGTases) synthesize cyclic oligosaccharides (cyclodextrins, CD) from starch. A CGTase from Bacillus sp. G‐825‐6 was engineered by site‐directed mutagenesis at two positions by the construction of the variants Y183W, Y183R, D358R, Y183W/D358R, and Y183R/D358...
Main Authors: | Christian Sonnendecker, Susanne Melzer, Wolfgang Zimmermann |
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Format: | Article |
Language: | English |
Published: |
Wiley
2019-06-01
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Series: | MicrobiologyOpen |
Subjects: | |
Online Access: | https://doi.org/10.1002/mbo3.757 |
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