Potential Acetylcholinesterase, Lipase, α-Glucosidase, and α-Amylase Inhibitory Activity, as well as Antimicrobial Activities, of Essential Oil from Lettuce Leaf Basil (<i>Ocimum basilicum</i> L.) Elicited with Jasmonic Acid

• Main Authors: Urszula Złotek, Kamila Rybczyńska-Tkaczyk, Monika Michalak-Majewska, Małgorzata Sikora, Anna Jakubczyk
• Format: Article
• Language: English
• Published: MDPI AG 2020-06-01
• Series: Applied Sciences
• Subjects: lettuce leaf basil (<i>Ocimum basilicum</i> L.), elicitation; essential oils; biological activities
• Online Access: https://www.mdpi.com/2076-3417/10/12/4315
• ISSN: 2076-3417

The aim of this study was to investigate the influence of the elicitation with jasmonic acid on the biological activities of essential oils (EOs) from lettuce leaf basil (<i>Ocimum basilicum</i> L.). Specifically, 0.01 µM jasmonic acid (JA1), 1 µM jasmonic acid (JA2), and 100 µM jasmonic acid (JA3) were used as elicitors. The results indicated that the elicitation increased the acetylcholinesterase, lipase, and α-amylase inhibitory activity of essential oils. A significant difference in α-glucosidase inhibition was noted only for the JA3 extract (IC₅₀ = 0.81 µL/mL), as this activity was lower than in the control sample without elicitation (IC₅₀ = 0.68 µL/mL). The studied basil EOs exhibited similar activity against <i>Staphylococcus aureus</i> (Gram-positive bacteria) and <i>Escherichia coli </i>(Gram-negative bacteria). Based on the value of the minimum inhibitory concentration (MIC) and the minimum bactericidal concentrations (MBC), the best antimicrobial activity was observed for JA2 and JA3.