Efficacy of Clonostachys rosea and Duddingtonia flagrans in Reducing the Haemonchus contortus Infective Larvae
The biocontrol is proven effective in reducing in vitro and in situ free-living stages of major gastrointestinal helminths, allowing progress in reducing losses by parasitism, maximizing production, and productivity. This study aimed at evaluating the predatory activity of fungal isolates of Dudding...
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doaj-a2cf60c56dd04489b8813ae2733edf5d2020-11-24T22:21:41ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/474879474879Efficacy of Clonostachys rosea and Duddingtonia flagrans in Reducing the Haemonchus contortus Infective LarvaeManoel Eduardo da Silva0Fabio Ribeiro Braga1Pedro Mendoza de Gives2Miguel Angel Mercado Uriostegui3Manuela Reyes4Filippe Elias de Freitas Soares5Lorendane Millena de Carvalho6Francielle Bosi Rodrigues7Jackson Victor de Araújo8Departamento de Veterinária, Universidade Federal de Viçosa, 36570000 Viçosa, MG, BrazilUniversidade Vila Velha, Vila Velha, ES, BrazilÁrea de Helmintologia, CENID-Parasitologia, INIFAP, Jiutepec, MOR, MexicoÁrea de Helmintologia, CENID-Parasitologia, INIFAP, Jiutepec, MOR, MexicoDepartamento de Microbiología Ambiental y Biotecnología, UAC, CAM, MexicoPrograma de Pós Graduação em Ciências Farmacêuticas (PPGF-UVV), Universidade Vila Velha, 29102920 Vila Velha, ES, BrazilDepartamento de Veterinária, Universidade Federal de Viçosa, 36570000 Viçosa, MG, BrazilPrograma de Pós Graduação em Ciências Farmacêuticas (PPGF-UVV), Universidade Vila Velha, 29102920 Vila Velha, ES, BrazilDepartamento de Veterinária, Universidade Federal de Viçosa, 36570000 Viçosa, MG, BrazilThe biocontrol is proven effective in reducing in vitro and in situ free-living stages of major gastrointestinal helminths, allowing progress in reducing losses by parasitism, maximizing production, and productivity. This study aimed at evaluating the predatory activity of fungal isolates of Duddingtonia flagrans and Clonostachys rosea species and its association on infective larvae (L3) of H. contortus in microplots formed by grasses and maintained in a protected environment. All groups were added with 10 mL of an aqueous suspension with 618 H. contortus L3 approximately. Group 1 was used as control and only received the infective larvae. Groups 2 and 3 received D. flagrans chlamydospores and C. rosea conidia at doses of 5 × 106. Group 4 received the combination of 5 × 106 D. flagrans chlamydospores + 5 × 106 C. rosea conidia. D. flagrans and C. rosea showed nematicidal effectiveness reducing by 91.5 and 88.9%, respectively, the population of H. contortus L3. However, when used in combination efficiency decreased to 74.5% predation of H. contortus L3. These results demonstrate the need for further studies to determine the existence of additive effects, synergistic or antagonistic, between these species.http://dx.doi.org/10.1155/2015/474879 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Manoel Eduardo da Silva Fabio Ribeiro Braga Pedro Mendoza de Gives Miguel Angel Mercado Uriostegui Manuela Reyes Filippe Elias de Freitas Soares Lorendane Millena de Carvalho Francielle Bosi Rodrigues Jackson Victor de Araújo |
spellingShingle |
Manoel Eduardo da Silva Fabio Ribeiro Braga Pedro Mendoza de Gives Miguel Angel Mercado Uriostegui Manuela Reyes Filippe Elias de Freitas Soares Lorendane Millena de Carvalho Francielle Bosi Rodrigues Jackson Victor de Araújo Efficacy of Clonostachys rosea and Duddingtonia flagrans in Reducing the Haemonchus contortus Infective Larvae BioMed Research International |
author_facet |
Manoel Eduardo da Silva Fabio Ribeiro Braga Pedro Mendoza de Gives Miguel Angel Mercado Uriostegui Manuela Reyes Filippe Elias de Freitas Soares Lorendane Millena de Carvalho Francielle Bosi Rodrigues Jackson Victor de Araújo |
author_sort |
Manoel Eduardo da Silva |
title |
Efficacy of Clonostachys rosea and Duddingtonia flagrans in Reducing the Haemonchus contortus Infective Larvae |
title_short |
Efficacy of Clonostachys rosea and Duddingtonia flagrans in Reducing the Haemonchus contortus Infective Larvae |
title_full |
Efficacy of Clonostachys rosea and Duddingtonia flagrans in Reducing the Haemonchus contortus Infective Larvae |
title_fullStr |
Efficacy of Clonostachys rosea and Duddingtonia flagrans in Reducing the Haemonchus contortus Infective Larvae |
title_full_unstemmed |
Efficacy of Clonostachys rosea and Duddingtonia flagrans in Reducing the Haemonchus contortus Infective Larvae |
title_sort |
efficacy of clonostachys rosea and duddingtonia flagrans in reducing the haemonchus contortus infective larvae |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2015-01-01 |
description |
The biocontrol is proven effective in reducing in vitro and in situ free-living stages of major gastrointestinal helminths, allowing progress in reducing losses by parasitism, maximizing production, and productivity. This study aimed at evaluating the predatory activity of fungal isolates of Duddingtonia flagrans and Clonostachys rosea species and its association on infective larvae (L3) of H. contortus in microplots formed by grasses and maintained in a protected environment. All groups were added with 10 mL of an aqueous suspension with 618 H. contortus L3 approximately. Group 1 was used as control and only received the infective larvae. Groups 2 and 3 received D. flagrans chlamydospores and C. rosea conidia at doses of 5 × 106. Group 4 received the combination of 5 × 106 D. flagrans chlamydospores + 5 × 106 C. rosea conidia. D. flagrans and C. rosea showed nematicidal effectiveness reducing by 91.5 and 88.9%, respectively, the population of H. contortus L3. However, when used in combination efficiency decreased to 74.5% predation of H. contortus L3. These results demonstrate the need for further studies to determine the existence of additive effects, synergistic or antagonistic, between these species. |
url |
http://dx.doi.org/10.1155/2015/474879 |
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