A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions

Protein-protein interactions (PPIs) play a major role in defining biological functions. Here, the authors present PPiSeq, a method to quantitatively score dynamic PPIs in yeast combining fragment complementation assays, genomic double-barcoding, and an analytical framework to precisely call fitness...

Full description

Bibliographic Details
Main Authors: Ulrich Schlecht, Zhimin Liu, Jamie R. Blundell, Robert P. St.Onge, Sasha F. Levy
Format: Article
Language:English
Published: Nature Publishing Group 2017-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms15586
Description
Summary:Protein-protein interactions (PPIs) play a major role in defining biological functions. Here, the authors present PPiSeq, a method to quantitatively score dynamic PPIs in yeast combining fragment complementation assays, genomic double-barcoding, and an analytical framework to precisely call fitness from barcode lineage trajectories.
ISSN:2041-1723