| Summary: | Plant response to salt stress and the mechanism of salt tolerance have received major focus by plant biology researchers. Biotic stresses cause extensive losses in agricultural production globally, but abiotic stress causes significant increase in the methylglyoxal (MG) level of <i>Glyoxalase</i><i>I</i> (<i>Gly I</i>). Identification of salt-tolerant genes when characterizing their phenotypes will help to identify novel genes using polymerase chain reaction (PCR) to amplify the DNA coding region for <i>glyoxalase I</i>. This method is specific, requiring only genomic DNA and two pairs of PCR primers, and involving two successive PCR reactions. This method was used rapidly and easily identified <i>glyoxalase I</i> sequences as salt-tolerant genes from Jojoba (<i>Simmondsia chinensis</i> (Link) Schneider). In the present study, the <i>glyoxalase I</i> gene was isolated, amplified by PCR using gene-specific primers and sequenced from the jojoba plant, then compared with other <i>glyoxalase I</i> sequences in other plants and <i>glyoxalase I</i> genes like in <i>Brassica napus</i>, ID: KT720495.1; <i>Brassica juncea</i> ID: Y13239.1, <i>Arachis hypogaea</i>; ID: DQ989209.2; and <i>Arabidopsis thaliana</i> L, ID: AAL84986. The structural gene of <i>glyoxalase I</i>, when sequenced and analyzed, revealed that the uninterrupted open reading frame (ORF) of jojoba <i>Gly I</i> (<i>Jojo-Gly I</i>) spans 775 bp, corresponding to 185 amino acid residues, and shares 45.2% amino acid sequence identity to jojoba (Jojo-Gly I). The cloned ORF, in a multicopy constitutive expression plasmid, complemented the <i>Jojo-Gly I</i>, confirming that the encoded <i>Jojo-Gly I</i> in jojoba showed some homology with other known <i>glyoxalase I</i> sequences of plants.
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