Anti-inflammatory effect of Moringa oleifera Lam. leaf extract on UVB-irradiated human keratinocytes

• Main Authors: Jinutda Engsuwan, Neti Waranuch, Nanteetip Limpeanchob, Kornkanok Ingkaninan
• Format: Article
• Language: English
• Published: Prince of Songkla University 2021-06-01
• Series: Songklanakarin Journal of Science and Technology (SJST)
• Subjects: moringa oleifera; astragalin; anti-inflammatory activity; primary human skin cells; uvb
• Online Access: https://rdo.psu.ac.th/sjstweb/journal/43-3/22.pdf
• ISSN: 0125-3395

Moringa oleifera Lam. has multiple biological properties that are applicable to developing cosmeceuticals. The objective of this study was to evaluate anti-inflammatory effects of M. oleifera on normal human keratinocytes. The antiinflammatory effects of water extract and 50% ethanol extract of M. oleifera leaves, and of the bioactive compound astragalin, on UVB-irradiated human keratinocytes were investigated. The levels of inflammatory mediators IL-1, IL-8, NO and PGE2 released from keratinocytes were measured using ELISA and Griess assays. UVB irradiation increased the release of IL-1, IL-8 and PGE2 into cell culture media after 24 h. The effect was significantly decreased by treatments with astragalin and M. oleifera leaf extracts, of which the 50% ethanol extract showed stronger inhibition than the water extract. These results suggest that this extract is a potential ingredient in products for relieving UVB-induced skin inflammation. Astragalin could be used as a bioactive positive control in the quality control of the extract.