A Fluorimetric Sensor for Detection of One Living Cell
Nowadays, studies of metabolic pathways and processes in living organisms cannot be easily done at the cellular level. That is why the development of a new analytical methods and approaches is needed, to allow detection of different biologically important species at very low concentrations levels an...
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MDPI AG
2007-03-01
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doaj-a32b34d2c144411e8f0ac416b153757a2020-11-24T22:24:41ZengMDPI AGSensors1424-82202007-03-017322223810.3390/s7030222A Fluorimetric Sensor for Detection of One Living CellRene KizekPetr BabulaKarl J. KramerLadislav HavelVojtech AdamJitka PetrlovaJan VitecekNowadays, studies of metabolic pathways and processes in living organisms cannot be easily done at the cellular level. That is why the development of a new analytical methods and approaches is needed, to allow detection of different biologically important species at very low concentrations levels and sample volumes, especially in individual cells. In the present work, we suggested a sensor to detect units of living cells by means determination of plant esterases (PE) based on fluorimetric detection of the products of the enzymatic hydrolysis of fluorescein diacetate in plant cell cultures (BY-2 tobacco cells and early somatic embryos of Norway spruce, clone 2/32). We standardized the sensor using a readily available esterase from pig liver. The detection limits were approximately 17 to 50 amol in 2 ml (8.5 to 25 femtomolar concentrations of esterases) of the enzyme contained in BY-2 tobacco cells and spruce early somatic embryos, respectively, after re-computation on the amounts of pig liver esterases. We assumed that the optimised sensor for the determination of PE in cell extracts accomplishes all requirements for a sensitive analysis which could be usable for single cell analysis. The detection limit was 1.5 in case of analysing BY-2 tobacco cells and 0.5 in early somatic embryos. Moreover, we were able to detect single protoplasts.http://www.mdpi.com/1424-8220/7/3/222/EsteraseEnzymesSingle-cell analysisAttomole detectionFluorimetryFluorescence microscopyConfocal microscopyNative gel electrophoresisFluorescein diacetateTobaccoBY-2 cellsProtoplastGrowth curveViability. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rene Kizek Petr Babula Karl J. Kramer Ladislav Havel Vojtech Adam Jitka Petrlova Jan Vitecek |
spellingShingle |
Rene Kizek Petr Babula Karl J. Kramer Ladislav Havel Vojtech Adam Jitka Petrlova Jan Vitecek A Fluorimetric Sensor for Detection of One Living Cell Sensors Esterase Enzymes Single-cell analysis Attomole detection Fluorimetry Fluorescence microscopy Confocal microscopy Native gel electrophoresis Fluorescein diacetate Tobacco BY-2 cells Protoplast Growth curve Viability. |
author_facet |
Rene Kizek Petr Babula Karl J. Kramer Ladislav Havel Vojtech Adam Jitka Petrlova Jan Vitecek |
author_sort |
Rene Kizek |
title |
A Fluorimetric Sensor for Detection of One Living Cell |
title_short |
A Fluorimetric Sensor for Detection of One Living Cell |
title_full |
A Fluorimetric Sensor for Detection of One Living Cell |
title_fullStr |
A Fluorimetric Sensor for Detection of One Living Cell |
title_full_unstemmed |
A Fluorimetric Sensor for Detection of One Living Cell |
title_sort |
fluorimetric sensor for detection of one living cell |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2007-03-01 |
description |
Nowadays, studies of metabolic pathways and processes in living organisms cannot be easily done at the cellular level. That is why the development of a new analytical methods and approaches is needed, to allow detection of different biologically important species at very low concentrations levels and sample volumes, especially in individual cells. In the present work, we suggested a sensor to detect units of living cells by means determination of plant esterases (PE) based on fluorimetric detection of the products of the enzymatic hydrolysis of fluorescein diacetate in plant cell cultures (BY-2 tobacco cells and early somatic embryos of Norway spruce, clone 2/32). We standardized the sensor using a readily available esterase from pig liver. The detection limits were approximately 17 to 50 amol in 2 ml (8.5 to 25 femtomolar concentrations of esterases) of the enzyme contained in BY-2 tobacco cells and spruce early somatic embryos, respectively, after re-computation on the amounts of pig liver esterases. We assumed that the optimised sensor for the determination of PE in cell extracts accomplishes all requirements for a sensitive analysis which could be usable for single cell analysis. The detection limit was 1.5 in case of analysing BY-2 tobacco cells and 0.5 in early somatic embryos. Moreover, we were able to detect single protoplasts. |
topic |
Esterase Enzymes Single-cell analysis Attomole detection Fluorimetry Fluorescence microscopy Confocal microscopy Native gel electrophoresis Fluorescein diacetate Tobacco BY-2 cells Protoplast Growth curve Viability. |
url |
http://www.mdpi.com/1424-8220/7/3/222/ |
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