Azithromycin attenuates airway inflammation in a mouse model of viral bronchiolitis
<p>Abstract</p> <p>Background</p> <p>Viral bronchiolitis is the leading cause of hospitalization in young infants. It is associated with the development of childhood asthma and contributes to morbidity and mortality in the elderly. Currently no therapies effectively att...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2010-06-01
|
Series: | Respiratory Research |
Online Access: | http://respiratory-research.com/content/11/1/90 |
id |
doaj-a32b35b29bae4aa6859c6449bd4fbf37 |
---|---|
record_format |
Article |
spelling |
doaj-a32b35b29bae4aa6859c6449bd4fbf372020-11-25T02:00:58ZengBMCRespiratory Research1465-99212010-06-011119010.1186/1465-9921-11-90Azithromycin attenuates airway inflammation in a mouse model of viral bronchiolitisBrody Steven LCannon Carolyn LGunsten Sean PMikols Cassandra LBeigelman AvrahamWalter Michael J<p>Abstract</p> <p>Background</p> <p>Viral bronchiolitis is the leading cause of hospitalization in young infants. It is associated with the development of childhood asthma and contributes to morbidity and mortality in the elderly. Currently no therapies effectively attenuate inflammation during the acute viral infection, or prevent the risk of post-viral asthma. We hypothesized that early treatment of a paramyxoviral bronchiolitis with azithromycin would attenuate acute and chronic airway inflammation.</p> <p>Methods</p> <p>Mice were inoculated with parainfluenza type 1, Sendai Virus (SeV), and treated daily with PBS or azithromycin for 7 days post-inoculation. On day 8 and 21 we assessed airway inflammation in lung tissue, and quantified immune cells and inflammatory mediators in bronchoalveolar lavage (BAL).</p> <p>Results</p> <p>Compared to treatment with PBS, azithromycin significantly attenuated post-viral weight loss. During the peak of acute inflammation (day 8), azithromycin decreased total leukocyte accumulation in the lung tissue and BAL, with the largest fold-reduction in BAL neutrophils. This decreased inflammation was independent of changes in viral load. Azithromycin significantly attenuated the concentration of BAL inflammatory mediators and enhanced resolution of chronic airway inflammation evident by decreased BAL inflammatory mediators on day 21.</p> <p>Conclusions</p> <p>In this mouse model of paramyxoviral bronchiolitis, azithromycin attenuated acute and chronic airway inflammation. These findings demonstrate anti-inflammatory effects of azithromycin that are not related to anti-viral activity. Our findings support the rationale for future prospective randomized clinical trials that will evaluate the effects of macrolides on acute viral bronchiolitis and their long-term consequences.</p> http://respiratory-research.com/content/11/1/90 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Brody Steven L Cannon Carolyn L Gunsten Sean P Mikols Cassandra L Beigelman Avraham Walter Michael J |
spellingShingle |
Brody Steven L Cannon Carolyn L Gunsten Sean P Mikols Cassandra L Beigelman Avraham Walter Michael J Azithromycin attenuates airway inflammation in a mouse model of viral bronchiolitis Respiratory Research |
author_facet |
Brody Steven L Cannon Carolyn L Gunsten Sean P Mikols Cassandra L Beigelman Avraham Walter Michael J |
author_sort |
Brody Steven L |
title |
Azithromycin attenuates airway inflammation in a mouse model of viral bronchiolitis |
title_short |
Azithromycin attenuates airway inflammation in a mouse model of viral bronchiolitis |
title_full |
Azithromycin attenuates airway inflammation in a mouse model of viral bronchiolitis |
title_fullStr |
Azithromycin attenuates airway inflammation in a mouse model of viral bronchiolitis |
title_full_unstemmed |
Azithromycin attenuates airway inflammation in a mouse model of viral bronchiolitis |
title_sort |
azithromycin attenuates airway inflammation in a mouse model of viral bronchiolitis |
publisher |
BMC |
series |
Respiratory Research |
issn |
1465-9921 |
publishDate |
2010-06-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Viral bronchiolitis is the leading cause of hospitalization in young infants. It is associated with the development of childhood asthma and contributes to morbidity and mortality in the elderly. Currently no therapies effectively attenuate inflammation during the acute viral infection, or prevent the risk of post-viral asthma. We hypothesized that early treatment of a paramyxoviral bronchiolitis with azithromycin would attenuate acute and chronic airway inflammation.</p> <p>Methods</p> <p>Mice were inoculated with parainfluenza type 1, Sendai Virus (SeV), and treated daily with PBS or azithromycin for 7 days post-inoculation. On day 8 and 21 we assessed airway inflammation in lung tissue, and quantified immune cells and inflammatory mediators in bronchoalveolar lavage (BAL).</p> <p>Results</p> <p>Compared to treatment with PBS, azithromycin significantly attenuated post-viral weight loss. During the peak of acute inflammation (day 8), azithromycin decreased total leukocyte accumulation in the lung tissue and BAL, with the largest fold-reduction in BAL neutrophils. This decreased inflammation was independent of changes in viral load. Azithromycin significantly attenuated the concentration of BAL inflammatory mediators and enhanced resolution of chronic airway inflammation evident by decreased BAL inflammatory mediators on day 21.</p> <p>Conclusions</p> <p>In this mouse model of paramyxoviral bronchiolitis, azithromycin attenuated acute and chronic airway inflammation. These findings demonstrate anti-inflammatory effects of azithromycin that are not related to anti-viral activity. Our findings support the rationale for future prospective randomized clinical trials that will evaluate the effects of macrolides on acute viral bronchiolitis and their long-term consequences.</p> |
url |
http://respiratory-research.com/content/11/1/90 |
work_keys_str_mv |
AT brodystevenl azithromycinattenuatesairwayinflammationinamousemodelofviralbronchiolitis AT cannoncarolynl azithromycinattenuatesairwayinflammationinamousemodelofviralbronchiolitis AT gunstenseanp azithromycinattenuatesairwayinflammationinamousemodelofviralbronchiolitis AT mikolscassandral azithromycinattenuatesairwayinflammationinamousemodelofviralbronchiolitis AT beigelmanavraham azithromycinattenuatesairwayinflammationinamousemodelofviralbronchiolitis AT waltermichaelj azithromycinattenuatesairwayinflammationinamousemodelofviralbronchiolitis |
_version_ |
1724959673651560448 |