Sucrose as a stable tracer for quantifying endodontic leakage

Background/purpose: Coronal or apical leakage is a major cause of endodontic treatment failure. To evaluate sucrose as a tracer for leakage testing for various filling materials involved in endodontic treatment. Materials and methods: The stability of sucrose and glucose was examined by immersing 11...

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Main Authors: Qiong Xu, Gary S.P. Cheung, Wen-Juan Deng, Jun-Qi Ling, Wei-Lan Li
Format: Article
Language:English
Published: Elsevier 2012-09-01
Series:Journal of Dental Sciences
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1991790212001134
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spelling doaj-a3346827dee8486d80ff3d1cc77a632f2020-11-24T23:52:18ZengElsevierJournal of Dental Sciences1991-79022012-09-017326727110.1016/j.jds.2012.06.002Sucrose as a stable tracer for quantifying endodontic leakageQiong Xu0Gary S.P. Cheung1Wen-Juan Deng2Jun-Qi Ling3Wei-Lan Li4Department of Conservative and Endodontic Dentistry, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, ChinaDepartment of Conservative and Endodontic Dentistry, University of Hong Kong, Hong Kong SAR, ChinaDepartment of Conservative and Endodontic Dentistry, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, ChinaDepartment of Conservative and Endodontic Dentistry, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, ChinaDepartment of Conservative and Endodontic Dentistry, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, ChinaBackground/purpose: Coronal or apical leakage is a major cause of endodontic treatment failure. To evaluate sucrose as a tracer for leakage testing for various filling materials involved in endodontic treatment. Materials and methods: The stability of sucrose and glucose was examined by immersing 11 common filling materials (1.5 × 3 mm blocks, cured for 1 week at 37°C in 100% relative humidity; n = 10 for each) in a 10mM solution of either sucrose or glucose. The concentration of the solution was measured after 1 week, 2 weeks, 4 weeks, and 8 weeks of immersion and compared. Then, the two tracers were used to test the sealing ability of zinc oxide–eugenol cement (IRM) and amalgam root-end fillings. Each material (n = 40 for each) was equally divided into two subgroups, these were evaluated with either glucose or sucrose as the tracer substance, and the amount of leakage was determined after 24 h, and 1 week, 2 weeks, 3 weeks, 4 weeks, 6 weeks, and 8 weeks. Results: Sucrose was stable with all materials at all time points. The concentration of glucose had significantly diminished after 1 week of immersion with mineral trioxide aggregate (MTA) or Sealapex (P < 0.001), but not with other filling materials (P > 0.05). Leakage results were similar (P > 0.05) when glucose and sucrose were used as the tracer substance, respectively. Amalgam leaked significantly less than IRM after 3 (sucrose test) and 4 weeks (glucose test) (P < 0.05). Conclusion: Sucrose appears to be stable in the presence of various endodontic materials, and can be used as a stable tracer substance for detecting endodontic microleakage.http://www.sciencedirect.com/science/article/pii/S1991790212001134endodontic microleakageglucoseroot-end fillingsucrose
collection DOAJ
language English
format Article
sources DOAJ
author Qiong Xu
Gary S.P. Cheung
Wen-Juan Deng
Jun-Qi Ling
Wei-Lan Li
spellingShingle Qiong Xu
Gary S.P. Cheung
Wen-Juan Deng
Jun-Qi Ling
Wei-Lan Li
Sucrose as a stable tracer for quantifying endodontic leakage
Journal of Dental Sciences
endodontic microleakage
glucose
root-end filling
sucrose
author_facet Qiong Xu
Gary S.P. Cheung
Wen-Juan Deng
Jun-Qi Ling
Wei-Lan Li
author_sort Qiong Xu
title Sucrose as a stable tracer for quantifying endodontic leakage
title_short Sucrose as a stable tracer for quantifying endodontic leakage
title_full Sucrose as a stable tracer for quantifying endodontic leakage
title_fullStr Sucrose as a stable tracer for quantifying endodontic leakage
title_full_unstemmed Sucrose as a stable tracer for quantifying endodontic leakage
title_sort sucrose as a stable tracer for quantifying endodontic leakage
publisher Elsevier
series Journal of Dental Sciences
issn 1991-7902
publishDate 2012-09-01
description Background/purpose: Coronal or apical leakage is a major cause of endodontic treatment failure. To evaluate sucrose as a tracer for leakage testing for various filling materials involved in endodontic treatment. Materials and methods: The stability of sucrose and glucose was examined by immersing 11 common filling materials (1.5 × 3 mm blocks, cured for 1 week at 37°C in 100% relative humidity; n = 10 for each) in a 10mM solution of either sucrose or glucose. The concentration of the solution was measured after 1 week, 2 weeks, 4 weeks, and 8 weeks of immersion and compared. Then, the two tracers were used to test the sealing ability of zinc oxide–eugenol cement (IRM) and amalgam root-end fillings. Each material (n = 40 for each) was equally divided into two subgroups, these were evaluated with either glucose or sucrose as the tracer substance, and the amount of leakage was determined after 24 h, and 1 week, 2 weeks, 3 weeks, 4 weeks, 6 weeks, and 8 weeks. Results: Sucrose was stable with all materials at all time points. The concentration of glucose had significantly diminished after 1 week of immersion with mineral trioxide aggregate (MTA) or Sealapex (P < 0.001), but not with other filling materials (P > 0.05). Leakage results were similar (P > 0.05) when glucose and sucrose were used as the tracer substance, respectively. Amalgam leaked significantly less than IRM after 3 (sucrose test) and 4 weeks (glucose test) (P < 0.05). Conclusion: Sucrose appears to be stable in the presence of various endodontic materials, and can be used as a stable tracer substance for detecting endodontic microleakage.
topic endodontic microleakage
glucose
root-end filling
sucrose
url http://www.sciencedirect.com/science/article/pii/S1991790212001134
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