Gene Expression Profiling and Pathway Network Analysis Predicts a Novel Antitumor Function for a Botanical-Derived Drug, PG2
PG2 is a botanical drug that is mostly composed of Astragalus polysaccharides (APS). Its role in hematopoiesis and relieving cancer-related fatigue has recently been clinically investigated in cancer patients. However, systematic analyses of its functions are still limited. The aim of this study was...
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doaj-a341c367d2d4427780a98b9c92c193392020-11-24T21:24:58ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882015-01-01201510.1155/2015/917345917345Gene Expression Profiling and Pathway Network Analysis Predicts a Novel Antitumor Function for a Botanical-Derived Drug, PG2Yu-Lun Kuo0Chun-Houh Chen1Tsung-Hsien Chuang2Wei-Kai Hua3Wey-Jinq Lin4Wei-Hsiang Hsu5Peter Mu-Hsin Chang6Shih-Lan Hsu7Tse-Hung Huang8Cheng-Yan Kao9Chi-Ying F. Huang10Department of Computer Science and Information Engineering, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 10617, TaiwanInstitute of Statistical Science, Academia Sinica, No. 128, Section 2, Academia Road, Nankang, Taipei 11529, TaiwanImmunology Research Center, National Health Research Institutes, No. 35, Keyan Road, Zhunan, Miaoli County 35053, TaiwanInstitute of Biopharmaceutical Sciences, National Yang-Ming University, No. 155, Section 2, Linong Street, Taipei 11221, TaiwanInstitute of Biopharmaceutical Sciences, National Yang-Ming University, No. 155, Section 2, Linong Street, Taipei 11221, TaiwanInstitute of Biopharmaceutical Sciences, National Yang-Ming University, No. 155, Section 2, Linong Street, Taipei 11221, TaiwanInstitute of Clinical Medicine, National Yang-Ming University, No. 155, Section 2, Linong Street, Taipei 11221, TaiwanDepartment of Education and Research, Taichung Veterans General Hospital, No. 1650 Taiwan Boulevard Section 4, Taichung 40705, TaiwanDepartment of Traditional Chinese Medicine, Chang Gung Memorial Hospital, No. 222, Maijin Road, Keelung 20401, TaiwanDepartment of Computer Science and Information Engineering, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 10617, TaiwanInstitute of Biopharmaceutical Sciences, National Yang-Ming University, No. 155, Section 2, Linong Street, Taipei 11221, TaiwanPG2 is a botanical drug that is mostly composed of Astragalus polysaccharides (APS). Its role in hematopoiesis and relieving cancer-related fatigue has recently been clinically investigated in cancer patients. However, systematic analyses of its functions are still limited. The aim of this study was to use microarray-based expression profiling to evaluate the quality and consistency of PG2 from three different product batches and to study biological mechanisms of PG2. An integrative molecular analysis approach has been designed to examine significant PG2-induced signatures in HL-60 leukemia cells. A quantitative analysis of gene expression signatures was conducted for PG2 by hierarchical clustering of correlation coefficients. The results showed that PG2 product batches were consistent and of high quality. These batches were also functionally equivalent to each other with regard to how they modulated the immune and hematopoietic systems. Within the PG2 signature, there were five genes associated with doxorubicin: IL-8, MDM4, BCL2, PRODH2, and BIRC5. Moreover, the combination of PG2 and doxorubicin had a synergistic effect on induced cell death in HL-60 cells. Together with the bioinformatics-based approach, gene expression profiling provided a quantitative measurement for the quality and consistency of herbal medicines and revealed new roles (e.g., immune modulation) for PG2 in cancer treatment.http://dx.doi.org/10.1155/2015/917345 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yu-Lun Kuo Chun-Houh Chen Tsung-Hsien Chuang Wei-Kai Hua Wey-Jinq Lin Wei-Hsiang Hsu Peter Mu-Hsin Chang Shih-Lan Hsu Tse-Hung Huang Cheng-Yan Kao Chi-Ying F. Huang |
spellingShingle |
Yu-Lun Kuo Chun-Houh Chen Tsung-Hsien Chuang Wei-Kai Hua Wey-Jinq Lin Wei-Hsiang Hsu Peter Mu-Hsin Chang Shih-Lan Hsu Tse-Hung Huang Cheng-Yan Kao Chi-Ying F. Huang Gene Expression Profiling and Pathway Network Analysis Predicts a Novel Antitumor Function for a Botanical-Derived Drug, PG2 Evidence-Based Complementary and Alternative Medicine |
author_facet |
Yu-Lun Kuo Chun-Houh Chen Tsung-Hsien Chuang Wei-Kai Hua Wey-Jinq Lin Wei-Hsiang Hsu Peter Mu-Hsin Chang Shih-Lan Hsu Tse-Hung Huang Cheng-Yan Kao Chi-Ying F. Huang |
author_sort |
Yu-Lun Kuo |
title |
Gene Expression Profiling and Pathway Network Analysis Predicts a Novel Antitumor Function for a Botanical-Derived Drug, PG2 |
title_short |
Gene Expression Profiling and Pathway Network Analysis Predicts a Novel Antitumor Function for a Botanical-Derived Drug, PG2 |
title_full |
Gene Expression Profiling and Pathway Network Analysis Predicts a Novel Antitumor Function for a Botanical-Derived Drug, PG2 |
title_fullStr |
Gene Expression Profiling and Pathway Network Analysis Predicts a Novel Antitumor Function for a Botanical-Derived Drug, PG2 |
title_full_unstemmed |
Gene Expression Profiling and Pathway Network Analysis Predicts a Novel Antitumor Function for a Botanical-Derived Drug, PG2 |
title_sort |
gene expression profiling and pathway network analysis predicts a novel antitumor function for a botanical-derived drug, pg2 |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2015-01-01 |
description |
PG2 is a botanical drug that is mostly composed of Astragalus polysaccharides (APS). Its role in hematopoiesis and relieving cancer-related fatigue has recently been clinically investigated in cancer patients. However, systematic analyses of its functions are still limited. The aim of this study was to use microarray-based expression profiling to evaluate the quality and consistency of PG2 from three different product batches and to study biological mechanisms of PG2. An integrative molecular analysis approach has been designed to examine significant PG2-induced signatures in HL-60 leukemia cells. A quantitative analysis of gene expression signatures was conducted for PG2 by hierarchical clustering of correlation coefficients. The results showed that PG2 product batches were consistent and of high quality. These batches were also functionally equivalent to each other with regard to how they modulated the immune and hematopoietic systems. Within the PG2 signature, there were five genes associated with doxorubicin: IL-8, MDM4, BCL2, PRODH2, and BIRC5. Moreover, the combination of PG2 and doxorubicin had a synergistic effect on induced cell death in HL-60 cells. Together with the bioinformatics-based approach, gene expression profiling provided a quantitative measurement for the quality and consistency of herbal medicines and revealed new roles (e.g., immune modulation) for PG2 in cancer treatment. |
url |
http://dx.doi.org/10.1155/2015/917345 |
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