Novel Polymer-Free Everolimus-Eluting Stent Fabricated using Femtosecond Laser Improves Re-endothelialization and Anti-inflammation
Abstract The aim of this study was to fabricate a novel polymer-free everolimus-eluting stent with nanostructure using a femtosecond laser (FSL). The stent were coated with everolimus (EVL) using FSL and electrospinning processes. The surface was rendered hydrophobic, which negatively affected both...
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doaj-a350853d57f14cf39c8d72c1c181b0af2020-12-08T03:54:12ZengNature Publishing GroupScientific Reports2045-23222018-05-018111110.1038/s41598-018-25629-9Novel Polymer-Free Everolimus-Eluting Stent Fabricated using Femtosecond Laser Improves Re-endothelialization and Anti-inflammationIn-Ho Bae0Myung Ho Jeong1Kyung Seob Lim2Dae Sung Park3Jae Won Shim4Jun-Kyu Park5Kwang Hwan Oh6Mi Rim Jin7Doo Sun Sim8Cardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and WelfareCardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and WelfareCardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and WelfareCardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and WelfareCardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and WelfareCardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and WelfareLaser Advanced System Industrialization Center, Jeonnam TechnoparkCardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and WelfareCardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and WelfareAbstract The aim of this study was to fabricate a novel polymer-free everolimus-eluting stent with nanostructure using a femtosecond laser (FSL). The stent were coated with everolimus (EVL) using FSL and electrospinning processes. The surface was rendered hydrophobic, which negatively affected both platelet adhesion (82.1%) and smooth muscle cell response. Animal study was performed using a porcine coronary restenosis model. The study groups were divided into 1) bare metal stent (BMS), 2) poly(L-lactide) (PLA)-based EVL drug eluting stent (DES), 3) commercial EVL-eluting DES, and 4) FSL-EVL-DES. After four weeks of stent implantation, various analyses were performed. Quantitative analysis showed that the amount of in-stent restenosis was higher in the BMS group (BMS; 27.8 ± 2.68%, PLA-based DES; 12.2 ± 0.57%, commercial DES; 9.8 ± 0.28%, and FSL-DES; 9.3 ± 0.25%, n = 10, p < 0.05). Specifically, the inflammation score was reduced in the FSL-DES group (1.9 ± 0.39, n = 10, p < 0.05). The increment in re-endothelialization in the FSL-DES group was confirmed by immunofluorescence analysis. Taken together, the novel polymer-free EVL-eluting stent fabricated using FSL can be an innovative DES with reduced risk of ISR, thrombosis, and inflammation.https://doi.org/10.1038/s41598-018-25629-9 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
In-Ho Bae Myung Ho Jeong Kyung Seob Lim Dae Sung Park Jae Won Shim Jun-Kyu Park Kwang Hwan Oh Mi Rim Jin Doo Sun Sim |
spellingShingle |
In-Ho Bae Myung Ho Jeong Kyung Seob Lim Dae Sung Park Jae Won Shim Jun-Kyu Park Kwang Hwan Oh Mi Rim Jin Doo Sun Sim Novel Polymer-Free Everolimus-Eluting Stent Fabricated using Femtosecond Laser Improves Re-endothelialization and Anti-inflammation Scientific Reports |
author_facet |
In-Ho Bae Myung Ho Jeong Kyung Seob Lim Dae Sung Park Jae Won Shim Jun-Kyu Park Kwang Hwan Oh Mi Rim Jin Doo Sun Sim |
author_sort |
In-Ho Bae |
title |
Novel Polymer-Free Everolimus-Eluting Stent Fabricated using Femtosecond Laser Improves Re-endothelialization and Anti-inflammation |
title_short |
Novel Polymer-Free Everolimus-Eluting Stent Fabricated using Femtosecond Laser Improves Re-endothelialization and Anti-inflammation |
title_full |
Novel Polymer-Free Everolimus-Eluting Stent Fabricated using Femtosecond Laser Improves Re-endothelialization and Anti-inflammation |
title_fullStr |
Novel Polymer-Free Everolimus-Eluting Stent Fabricated using Femtosecond Laser Improves Re-endothelialization and Anti-inflammation |
title_full_unstemmed |
Novel Polymer-Free Everolimus-Eluting Stent Fabricated using Femtosecond Laser Improves Re-endothelialization and Anti-inflammation |
title_sort |
novel polymer-free everolimus-eluting stent fabricated using femtosecond laser improves re-endothelialization and anti-inflammation |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2018-05-01 |
description |
Abstract The aim of this study was to fabricate a novel polymer-free everolimus-eluting stent with nanostructure using a femtosecond laser (FSL). The stent were coated with everolimus (EVL) using FSL and electrospinning processes. The surface was rendered hydrophobic, which negatively affected both platelet adhesion (82.1%) and smooth muscle cell response. Animal study was performed using a porcine coronary restenosis model. The study groups were divided into 1) bare metal stent (BMS), 2) poly(L-lactide) (PLA)-based EVL drug eluting stent (DES), 3) commercial EVL-eluting DES, and 4) FSL-EVL-DES. After four weeks of stent implantation, various analyses were performed. Quantitative analysis showed that the amount of in-stent restenosis was higher in the BMS group (BMS; 27.8 ± 2.68%, PLA-based DES; 12.2 ± 0.57%, commercial DES; 9.8 ± 0.28%, and FSL-DES; 9.3 ± 0.25%, n = 10, p < 0.05). Specifically, the inflammation score was reduced in the FSL-DES group (1.9 ± 0.39, n = 10, p < 0.05). The increment in re-endothelialization in the FSL-DES group was confirmed by immunofluorescence analysis. Taken together, the novel polymer-free EVL-eluting stent fabricated using FSL can be an innovative DES with reduced risk of ISR, thrombosis, and inflammation. |
url |
https://doi.org/10.1038/s41598-018-25629-9 |
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