A reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient models
Abstract CRISPR/Cas9 represents a valuable tool to determine protein function, but technical hurdles limit its use in challenging settings such as cells unable to grow in vitro like primary leukemia cells and xenografts derived thereof (PDX). To enrich CRISPR/Cas9-edited cells, we improved a dual-re...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2021-06-01
|
Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-021-91760-9 |
id |
doaj-a377dc79c39245ce948428dc257d1833 |
---|---|
record_format |
Article |
spelling |
doaj-a377dc79c39245ce948428dc257d18332021-06-20T11:34:22ZengNature Publishing GroupScientific Reports2045-23222021-06-011111810.1038/s41598-021-91760-9A reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient modelsWen-Hsin Liu0Kerstin Völse1Daniela Senft2Irmela Jeremias3Research Unit Apoptosis in Hematopoietic Stem Cells, German Research Center for Environmental Health (HMGU), Helmholtz Zentrum MünchenResearch Unit Apoptosis in Hematopoietic Stem Cells, German Research Center for Environmental Health (HMGU), Helmholtz Zentrum MünchenResearch Unit Apoptosis in Hematopoietic Stem Cells, German Research Center for Environmental Health (HMGU), Helmholtz Zentrum MünchenResearch Unit Apoptosis in Hematopoietic Stem Cells, German Research Center for Environmental Health (HMGU), Helmholtz Zentrum MünchenAbstract CRISPR/Cas9 represents a valuable tool to determine protein function, but technical hurdles limit its use in challenging settings such as cells unable to grow in vitro like primary leukemia cells and xenografts derived thereof (PDX). To enrich CRISPR/Cas9-edited cells, we improved a dual-reporter system and cloned the genomic target sequences of the gene of interest (GOI) upstream of an out-of-frame fluorochrome which was expressed only upon successful gene editing. To reduce rounds of in vivo passaging required for PDX leukemia growth, targets of 17 GOI were cloned in a row, flanked by an improved linker, and PDX cells were lentivirally transduced for stable expression. The reporter enriched scarce, successfully gene-edited PDX cells as high as 80%. Using the reporter, we show that KO of the SRC-family kinase LYN increased the response of PDX cells of B precursor cell ALL towards Vincristine, even upon heterozygous KO, indicating haploinsufficiency. In summary, our reporter system enables enriching KO cells in technically challenging settings and extends the use of gene editing to highly patient-related model systems.https://doi.org/10.1038/s41598-021-91760-9 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wen-Hsin Liu Kerstin Völse Daniela Senft Irmela Jeremias |
spellingShingle |
Wen-Hsin Liu Kerstin Völse Daniela Senft Irmela Jeremias A reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient models Scientific Reports |
author_facet |
Wen-Hsin Liu Kerstin Völse Daniela Senft Irmela Jeremias |
author_sort |
Wen-Hsin Liu |
title |
A reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient models |
title_short |
A reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient models |
title_full |
A reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient models |
title_fullStr |
A reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient models |
title_full_unstemmed |
A reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient models |
title_sort |
reporter system for enriching crispr/cas9 knockout cells in technically challenging settings like patient models |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-06-01 |
description |
Abstract CRISPR/Cas9 represents a valuable tool to determine protein function, but technical hurdles limit its use in challenging settings such as cells unable to grow in vitro like primary leukemia cells and xenografts derived thereof (PDX). To enrich CRISPR/Cas9-edited cells, we improved a dual-reporter system and cloned the genomic target sequences of the gene of interest (GOI) upstream of an out-of-frame fluorochrome which was expressed only upon successful gene editing. To reduce rounds of in vivo passaging required for PDX leukemia growth, targets of 17 GOI were cloned in a row, flanked by an improved linker, and PDX cells were lentivirally transduced for stable expression. The reporter enriched scarce, successfully gene-edited PDX cells as high as 80%. Using the reporter, we show that KO of the SRC-family kinase LYN increased the response of PDX cells of B precursor cell ALL towards Vincristine, even upon heterozygous KO, indicating haploinsufficiency. In summary, our reporter system enables enriching KO cells in technically challenging settings and extends the use of gene editing to highly patient-related model systems. |
url |
https://doi.org/10.1038/s41598-021-91760-9 |
work_keys_str_mv |
AT wenhsinliu areportersystemforenrichingcrisprcas9knockoutcellsintechnicallychallengingsettingslikepatientmodels AT kerstinvolse areportersystemforenrichingcrisprcas9knockoutcellsintechnicallychallengingsettingslikepatientmodels AT danielasenft areportersystemforenrichingcrisprcas9knockoutcellsintechnicallychallengingsettingslikepatientmodels AT irmelajeremias areportersystemforenrichingcrisprcas9knockoutcellsintechnicallychallengingsettingslikepatientmodels AT wenhsinliu reportersystemforenrichingcrisprcas9knockoutcellsintechnicallychallengingsettingslikepatientmodels AT kerstinvolse reportersystemforenrichingcrisprcas9knockoutcellsintechnicallychallengingsettingslikepatientmodels AT danielasenft reportersystemforenrichingcrisprcas9knockoutcellsintechnicallychallengingsettingslikepatientmodels AT irmelajeremias reportersystemforenrichingcrisprcas9knockoutcellsintechnicallychallengingsettingslikepatientmodels |
_version_ |
1721369901539524608 |