Investigating Effects of Superparamagnetic Iron Oxide Nanoparticles on Candida albicans Biofilm Formation
ABSTRACT Background and Objectives: Candida albicans is one of the most common fungal pathogens that can form biofilm, particularly on surface of medical devices. In recent years, C. albicans has shown increased resistance to antifungal agents. In this experimental study, we aimed to stud...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Golestan University of Medical Sciences
2019-11-01
|
Series: | Medical Laboratory Journal |
Subjects: | |
Online Access: | http://mlj.goums.ac.ir/article-1-1164-en.html |
id |
doaj-a3b4bcf88e18433db44215d8b1f4f26f |
---|---|
record_format |
Article |
spelling |
doaj-a3b4bcf88e18433db44215d8b1f4f26f2021-09-01T13:20:26ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492019-11-011364450Investigating Effects of Superparamagnetic Iron Oxide Nanoparticles on Candida albicans Biofilm FormationFateme Golipour0Reza Habibipour1Leila Moradihaghgou2 Hamedan branch,Islamic Azad University,Hamedan, Iran Hamedan branch,Islamic Azad University,Hamedan, Iran Hamedan branch,Islamic Azad University,Hamedan, Iran ABSTRACT Background and Objectives: Candida albicans is one of the most common fungal pathogens that can form biofilm, particularly on surface of medical devices. In recent years, C. albicans has shown increased resistance to antifungal agents. In this experimental study, we aimed to study effects of superparamagnetic iron oxide nanoparticles (Fe3O4 nanoparticles or SPION) on biofilm formation by C. albicans. Methods: First, the SPION were synthesized by chemical co-precipitation. The formation of nanoparticles was confirmed by Fourier-transform infrared spectroscopy and X-ray diffraction. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of SPION were determined. Then, antibiofilm effects of the nanoparticles were investigated by enzyme-linked immunosorbent assay. Finally, data were analyzed using SPSS 22.0 at significance level of 0.05. Results: According to the results of X-ray diffraction, the SPION had a mean diameter of about 70 nm. MIC and MFC values of SPION against C. albicans were 100 ppm and 200 ppm which reduced biofilm formation by 87.2% and 100%, respectively. SPION showed significant inhibitory effects on C. albicans growth and biofilm formation. Conclusion: Based on the findings, SPION may be considered as a novel family of fungicidal compounds. However, further studies are necessary to evaluate the safety of these nanoparticles for treatment of fungal infections in humans. Keywords: Candida albicans; Biofilms; SPION; Nanoparticles.http://mlj.goums.ac.ir/article-1-1164-en.html: candida albicansbiofilmspionnanoparticles |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fateme Golipour Reza Habibipour Leila Moradihaghgou |
spellingShingle |
Fateme Golipour Reza Habibipour Leila Moradihaghgou Investigating Effects of Superparamagnetic Iron Oxide Nanoparticles on Candida albicans Biofilm Formation Medical Laboratory Journal : candida albicans biofilm spion nanoparticles |
author_facet |
Fateme Golipour Reza Habibipour Leila Moradihaghgou |
author_sort |
Fateme Golipour |
title |
Investigating Effects of Superparamagnetic Iron Oxide Nanoparticles on Candida albicans Biofilm Formation |
title_short |
Investigating Effects of Superparamagnetic Iron Oxide Nanoparticles on Candida albicans Biofilm Formation |
title_full |
Investigating Effects of Superparamagnetic Iron Oxide Nanoparticles on Candida albicans Biofilm Formation |
title_fullStr |
Investigating Effects of Superparamagnetic Iron Oxide Nanoparticles on Candida albicans Biofilm Formation |
title_full_unstemmed |
Investigating Effects of Superparamagnetic Iron Oxide Nanoparticles on Candida albicans Biofilm Formation |
title_sort |
investigating effects of superparamagnetic iron oxide nanoparticles on candida albicans biofilm formation |
publisher |
Golestan University of Medical Sciences |
series |
Medical Laboratory Journal |
issn |
2538-4449 |
publishDate |
2019-11-01 |
description |
ABSTRACT
Background and Objectives: Candida albicans is one of the most common fungal pathogens that can form biofilm, particularly on surface of medical devices. In recent years, C. albicans has shown increased resistance to antifungal agents. In this experimental study, we aimed to study effects of superparamagnetic iron oxide nanoparticles (Fe3O4 nanoparticles or SPION) on biofilm formation by C. albicans.
Methods: First, the SPION were synthesized by chemical co-precipitation. The formation of nanoparticles was confirmed by Fourier-transform infrared spectroscopy and X-ray diffraction. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of SPION were determined. Then, antibiofilm effects of the nanoparticles were investigated by enzyme-linked immunosorbent assay. Finally, data were analyzed using SPSS 22.0 at significance level of 0.05.
Results: According to the results of X-ray diffraction, the SPION had a mean diameter of about 70 nm. MIC and MFC values of SPION against C. albicans were 100 ppm and 200 ppm which reduced biofilm formation by 87.2% and 100%, respectively. SPION showed significant inhibitory effects on C. albicans growth and biofilm formation.
Conclusion: Based on the findings, SPION may be considered as a novel family of fungicidal compounds. However, further studies are necessary to evaluate the safety of these nanoparticles for treatment of fungal infections in humans.
Keywords: Candida albicans; Biofilms; SPION; Nanoparticles. |
topic |
: candida albicans biofilm spion nanoparticles |
url |
http://mlj.goums.ac.ir/article-1-1164-en.html |
work_keys_str_mv |
AT fatemegolipour investigatingeffectsofsuperparamagneticironoxidenanoparticlesoncandidaalbicansbiofilmformation AT rezahabibipour investigatingeffectsofsuperparamagneticironoxidenanoparticlesoncandidaalbicansbiofilmformation AT leilamoradihaghgou investigatingeffectsofsuperparamagneticironoxidenanoparticlesoncandidaalbicansbiofilmformation |
_version_ |
1724162932896432128 |