Rapid Mitochondrial DNA Segregation in Primate Preimplantation Embryos Precedes Somatic and Germline Bottleneck
The timing and mechanisms of mitochondrial DNA (mtDNA) segregation and transmission in mammals are poorly understood. Genetic bottleneck in female germ cells has been proposed as the main phenomenon responsible for rapid intergenerational segregation of heteroplasmic mtDNA. We demonstrate here that...
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doaj-a3c7b267db3540098afb9f50e3ed43d62020-11-24T21:59:55ZengElsevierCell Reports2211-12472012-05-011550651510.1016/j.celrep.2012.03.011Rapid Mitochondrial DNA Segregation in Primate Preimplantation Embryos Precedes Somatic and Germline BottleneckHyo-Sang Lee0Hong Ma1Rita Cervera Juanes2Masahito Tachibana3Michelle Sparman4Joy Woodward5Cathy Ramsey6Jing Xu7Eun-Ju Kang8Paula Amato9Georg Mair10Ralf Steinborn11Shoukhrat Mitalipov12Division of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR 97006, USADivision of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR 97006, USADivision of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR 97006, USADivision of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR 97006, USADivision of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR 97006, USADivision of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR 97006, USADivision of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR 97006, USADivision of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR 97006, USADivision of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR 97006, USADepartments of Obstetrics & Gynecology, Oregon Health & Science University, Beaverton, OR 97006, USAVetCore Facility for Research VetOmics, University of Veterinary Medicine, 1210 Vienna, AustriaVetCore Facility for Research VetOmics, University of Veterinary Medicine, 1210 Vienna, AustriaDivision of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR 97006, USA The timing and mechanisms of mitochondrial DNA (mtDNA) segregation and transmission in mammals are poorly understood. Genetic bottleneck in female germ cells has been proposed as the main phenomenon responsible for rapid intergenerational segregation of heteroplasmic mtDNA. We demonstrate here that mtDNA segregation occurs during primate preimplantation embryogenesis resulting in partitioning of mtDNA variants between daughter blastomeres. A substantial shift toward homoplasmy occurred in fetuses and embryonic stem cells (ESCs) derived from these heteroplasmic embryos. We also observed a wide range of heteroplasmic mtDNA variants distributed in individual oocytes recovered from these fetuses. Thus, we present here evidence for a previously unknown mtDNA segregation and bottleneck during preimplantation embryo development, suggesting that return to the homoplasmic condition can occur during development of an individual organism from the zygote to birth, without a passage through the germline. http://www.sciencedirect.com/science/article/pii/S2211124712000964 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hyo-Sang Lee Hong Ma Rita Cervera Juanes Masahito Tachibana Michelle Sparman Joy Woodward Cathy Ramsey Jing Xu Eun-Ju Kang Paula Amato Georg Mair Ralf Steinborn Shoukhrat Mitalipov |
spellingShingle |
Hyo-Sang Lee Hong Ma Rita Cervera Juanes Masahito Tachibana Michelle Sparman Joy Woodward Cathy Ramsey Jing Xu Eun-Ju Kang Paula Amato Georg Mair Ralf Steinborn Shoukhrat Mitalipov Rapid Mitochondrial DNA Segregation in Primate Preimplantation Embryos Precedes Somatic and Germline Bottleneck Cell Reports |
author_facet |
Hyo-Sang Lee Hong Ma Rita Cervera Juanes Masahito Tachibana Michelle Sparman Joy Woodward Cathy Ramsey Jing Xu Eun-Ju Kang Paula Amato Georg Mair Ralf Steinborn Shoukhrat Mitalipov |
author_sort |
Hyo-Sang Lee |
title |
Rapid Mitochondrial DNA Segregation in Primate Preimplantation Embryos Precedes Somatic and Germline Bottleneck |
title_short |
Rapid Mitochondrial DNA Segregation in Primate Preimplantation Embryos Precedes Somatic and Germline Bottleneck |
title_full |
Rapid Mitochondrial DNA Segregation in Primate Preimplantation Embryos Precedes Somatic and Germline Bottleneck |
title_fullStr |
Rapid Mitochondrial DNA Segregation in Primate Preimplantation Embryos Precedes Somatic and Germline Bottleneck |
title_full_unstemmed |
Rapid Mitochondrial DNA Segregation in Primate Preimplantation Embryos Precedes Somatic and Germline Bottleneck |
title_sort |
rapid mitochondrial dna segregation in primate preimplantation embryos precedes somatic and germline bottleneck |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2012-05-01 |
description |
The timing and mechanisms of mitochondrial DNA (mtDNA) segregation and transmission in mammals are poorly understood. Genetic bottleneck in female germ cells has been proposed as the main phenomenon responsible for rapid intergenerational segregation of heteroplasmic mtDNA. We demonstrate here that mtDNA segregation occurs during primate preimplantation embryogenesis resulting in partitioning of mtDNA variants between daughter blastomeres. A substantial shift toward homoplasmy occurred in fetuses and embryonic stem cells (ESCs) derived from these heteroplasmic embryos. We also observed a wide range of heteroplasmic mtDNA variants distributed in individual oocytes recovered from these fetuses. Thus, we present here evidence for a previously unknown mtDNA segregation and bottleneck during preimplantation embryo development, suggesting that return to the homoplasmic condition can occur during development of an individual organism from the zygote to birth, without a passage through the germline.
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url |
http://www.sciencedirect.com/science/article/pii/S2211124712000964 |
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