Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical Applications

• Main Authors: Y. S. Tapia-Guerrero, M. L. Del Prado-Audelo, F. V. Borbolla-Jiménez, D. M. Giraldo Gomez, I. García-Aguirre, C. A. Colín-Castro, J. A. Morales-González, G. Leyva-Gómez, J. J. Magaña
• Format: Article
• Language: English
• Published: MDPI AG 2020-03-01
• Series: Materials
• Subjects: nanoparticles; sterilization; ultraviolet radiation; gamma radiation; poly (<span style="font-variant: small-caps">d</span>,<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) acid; poly-(ε-caprolactone)
• Online Access: https://www.mdpi.com/1996-1944/13/5/1090

The sterilization processes of nanoparticles (NP) by autoclaving and filtration are two of the most utilized methods in the pharmaceutical industry but are not always a viable option. For this reason, the search for alternative options such as UV and gamma radiation is of interest. In this work, we evaluated both types of sterilization on two types of NP in solid state widely employed in the literature for biomedical applications, poly-(&#949;-caprolactone) and poly(<span style="font-variant: small-caps;">d</span>,<span style="font-variant: small-caps;">l</span>-lactide-<i>co</i>-glycolide) acid NP stabilized with polyvinyl alcohol. Physicochemical properties and cell viability were studied pre- and post-sterilization. The efficiency of irradiation sterilization was performed by a test of sterility using 1 &#215; 10⁸ CFU/mL of <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, and <i>Candida albicans</i>. Microbiological monitoring revealed that both methods were sufficient for sterilization. After the UV irradiation sterilization (100 &#181;J/cm²), no substantial changes were observed in the physicochemical properties of the NP or in the interaction or morphology of human glial cells, though 5 and 10 kGy of gamma irradiation showed slight changes of NP size as well as a decrease in cell viability (from 100 &#181;g/mL of NP). At 5 kGy of radiation doses, the presence of trehalose as cryoprotectant reduces the cell damage with high concentrations of NP, but this did not occur at 10 kGy. Therefore, these methods could be highly effective and low-processing-time options for sterilizing NP for medical purposes. However, we suggest validating each NP system because these generally are of different polymer-composition systems.