Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical Applications
The sterilization processes of nanoparticles (NP) by autoclaving and filtration are two of the most utilized methods in the pharmaceutical industry but are not always a viable option. For this reason, the search for alternative options such as UV and gamma radiation is of interest. In this work, we...
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doaj-a3e79bc9b2464ea295af80067443138a2020-11-25T00:32:38ZengMDPI AGMaterials1996-19442020-03-01135109010.3390/ma13051090ma13051090Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical ApplicationsY. S. Tapia-Guerrero0M. L. Del Prado-Audelo1F. V. Borbolla-Jiménez2D. M. Giraldo Gomez3I. García-Aguirre4C. A. Colín-Castro5J. A. Morales-González6G. Leyva-Gómez7J. J. Magaña8Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón, Ciudad de México 11340, MexicoLaboratorio de Tecnología Farmacéutica, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Cuautitlán Izcalli, Edo. de México 54740, MexicoLaboratorio de Medicina Genómica, Departamento de Genética (CENIAQ), Instituto Nacional de Rehabilitación-Luis Guillermo Ibarra Ibarra (INR-LGII), Ciudad de México 14389, MexicoDepartamento de Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Edificio “A” 3er piso, Circuito Interior, Avenida Universidad 3000, Ciudad Universitaria, Coyoacán, Ciudad de México 04510, MexicoDepartamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), Ciudad de México 07360, MexicoLaboratorio de Medicina Genómica, Departamento de Genética (CENIAQ), Instituto Nacional de Rehabilitación-Luis Guillermo Ibarra Ibarra (INR-LGII), Ciudad de México 14389, MexicoSección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón, Ciudad de México 11340, MexicoDepartamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México 04510, MexicoSección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón, Ciudad de México 11340, MexicoThe sterilization processes of nanoparticles (NP) by autoclaving and filtration are two of the most utilized methods in the pharmaceutical industry but are not always a viable option. For this reason, the search for alternative options such as UV and gamma radiation is of interest. In this work, we evaluated both types of sterilization on two types of NP in solid state widely employed in the literature for biomedical applications, poly-(ε-caprolactone) and poly(<span style="font-variant: small-caps;">d</span>,<span style="font-variant: small-caps;">l</span>-lactide-<i>co</i>-glycolide) acid NP stabilized with polyvinyl alcohol. Physicochemical properties and cell viability were studied pre- and post-sterilization. The efficiency of irradiation sterilization was performed by a test of sterility using 1 × 10<sup>8</sup> CFU/mL of <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, and <i>Candida albicans</i>. Microbiological monitoring revealed that both methods were sufficient for sterilization. After the UV irradiation sterilization (100 µJ/cm<sup>2</sup>), no substantial changes were observed in the physicochemical properties of the NP or in the interaction or morphology of human glial cells, though 5 and 10 kGy of gamma irradiation showed slight changes of NP size as well as a decrease in cell viability (from 100 µg/mL of NP). At 5 kGy of radiation doses, the presence of trehalose as cryoprotectant reduces the cell damage with high concentrations of NP, but this did not occur at 10 kGy. Therefore, these methods could be highly effective and low-processing-time options for sterilizing NP for medical purposes. However, we suggest validating each NP system because these generally are of different polymer-composition systems.https://www.mdpi.com/1996-1944/13/5/1090nanoparticlessterilizationultraviolet radiationgamma radiationpoly (<span style="font-variant: small-caps">d</span>,<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) acidpoly-(ε-caprolactone) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Y. S. Tapia-Guerrero M. L. Del Prado-Audelo F. V. Borbolla-Jiménez D. M. Giraldo Gomez I. García-Aguirre C. A. Colín-Castro J. A. Morales-González G. Leyva-Gómez J. J. Magaña |
spellingShingle |
Y. S. Tapia-Guerrero M. L. Del Prado-Audelo F. V. Borbolla-Jiménez D. M. Giraldo Gomez I. García-Aguirre C. A. Colín-Castro J. A. Morales-González G. Leyva-Gómez J. J. Magaña Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical Applications Materials nanoparticles sterilization ultraviolet radiation gamma radiation poly (<span style="font-variant: small-caps">d</span>,<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) acid poly-(ε-caprolactone) |
author_facet |
Y. S. Tapia-Guerrero M. L. Del Prado-Audelo F. V. Borbolla-Jiménez D. M. Giraldo Gomez I. García-Aguirre C. A. Colín-Castro J. A. Morales-González G. Leyva-Gómez J. J. Magaña |
author_sort |
Y. S. Tapia-Guerrero |
title |
Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical Applications |
title_short |
Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical Applications |
title_full |
Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical Applications |
title_fullStr |
Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical Applications |
title_full_unstemmed |
Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical Applications |
title_sort |
effect of uv and gamma irradiation sterilization processes in the properties of different polymeric nanoparticles for biomedical applications |
publisher |
MDPI AG |
series |
Materials |
issn |
1996-1944 |
publishDate |
2020-03-01 |
description |
The sterilization processes of nanoparticles (NP) by autoclaving and filtration are two of the most utilized methods in the pharmaceutical industry but are not always a viable option. For this reason, the search for alternative options such as UV and gamma radiation is of interest. In this work, we evaluated both types of sterilization on two types of NP in solid state widely employed in the literature for biomedical applications, poly-(ε-caprolactone) and poly(<span style="font-variant: small-caps;">d</span>,<span style="font-variant: small-caps;">l</span>-lactide-<i>co</i>-glycolide) acid NP stabilized with polyvinyl alcohol. Physicochemical properties and cell viability were studied pre- and post-sterilization. The efficiency of irradiation sterilization was performed by a test of sterility using 1 × 10<sup>8</sup> CFU/mL of <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, and <i>Candida albicans</i>. Microbiological monitoring revealed that both methods were sufficient for sterilization. After the UV irradiation sterilization (100 µJ/cm<sup>2</sup>), no substantial changes were observed in the physicochemical properties of the NP or in the interaction or morphology of human glial cells, though 5 and 10 kGy of gamma irradiation showed slight changes of NP size as well as a decrease in cell viability (from 100 µg/mL of NP). At 5 kGy of radiation doses, the presence of trehalose as cryoprotectant reduces the cell damage with high concentrations of NP, but this did not occur at 10 kGy. Therefore, these methods could be highly effective and low-processing-time options for sterilizing NP for medical purposes. However, we suggest validating each NP system because these generally are of different polymer-composition systems. |
topic |
nanoparticles sterilization ultraviolet radiation gamma radiation poly (<span style="font-variant: small-caps">d</span>,<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) acid poly-(ε-caprolactone) |
url |
https://www.mdpi.com/1996-1944/13/5/1090 |
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